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Title: Repression of jasmonate signaling by a non‐TIFY JAZ protein in Arabidopsis

Abstract

Summary JAsmonate ZIM‐domain (JAZ) proteins repress the activity of transcription factors that execute responses to the plant hormone jasmonoyl‐L‐isoleucine (JA‐Ile). The ZIM protein domain recruits the co‐repressors NINJA and TOPLESS to JAZ‐bound transcription factors, and contains a highly conserved TIF[F/Y]XG motif that defines the larger family of TIFY proteins to which JAZs belong. Here, we report that diverse plant species contain genes encoding putative non‐TIFY JAZ proteins, including a previously unrecognized JAZ repressor in Arabidopsis (JAZ13, encoded by At3g22275). JAZ13 is most closely related to JAZ8 and includes divergent EAR, TIFY/ZIM, and Jas motifs. Unlike JAZ8, however, JAZ13 contains a Ser‐rich C‐terminal tail that is a site for phosphorylation. Overexpression of JAZ13 resulted in reduced sensitivity to JA, attenuation of wound‐induced expression of JA‐response genes, and decreased resistance to insect herbivory. JAZ13 interacts with the bHLH transcription factor MYC2 and the co‐repressor TOPLESS but, consistent with the absence of a TIFY motif, neither NINJA nor other JAZs. Analysis of single and higher‐order T‐DNA insertion jaz null mutants provided further evidence that JAZ13 is a repressor JA signaling. Our results demonstrate that proteins outside the TIFY family are functional JAZ repressors and further suggest that this expansion of the JAZ familymore » allows fine‐tuning of JA‐mediated transcriptional responses.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA
  2. Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI 48824 USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1401134
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Name: The Plant Journal Journal Volume: 82 Journal Issue: 4; Journal ID: ISSN 0960-7412
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Thireault, Caitlin, Shyu, Christine, Yoshida, Yuki, St. Aubin, Brian, Campos, Marcelo L., and Howe, Gregg A. Repression of jasmonate signaling by a non‐TIFY JAZ protein in Arabidopsis. United Kingdom: N. p., 2015. Web. doi:10.1111/tpj.12841.
Thireault, Caitlin, Shyu, Christine, Yoshida, Yuki, St. Aubin, Brian, Campos, Marcelo L., & Howe, Gregg A. Repression of jasmonate signaling by a non‐TIFY JAZ protein in Arabidopsis. United Kingdom. https://doi.org/10.1111/tpj.12841
Thireault, Caitlin, Shyu, Christine, Yoshida, Yuki, St. Aubin, Brian, Campos, Marcelo L., and Howe, Gregg A. Sat . "Repression of jasmonate signaling by a non‐TIFY JAZ protein in Arabidopsis". United Kingdom. https://doi.org/10.1111/tpj.12841.
@article{osti_1401134,
title = {Repression of jasmonate signaling by a non‐TIFY JAZ protein in Arabidopsis},
author = {Thireault, Caitlin and Shyu, Christine and Yoshida, Yuki and St. Aubin, Brian and Campos, Marcelo L. and Howe, Gregg A.},
abstractNote = {Summary JAsmonate ZIM‐domain (JAZ) proteins repress the activity of transcription factors that execute responses to the plant hormone jasmonoyl‐L‐isoleucine (JA‐Ile). The ZIM protein domain recruits the co‐repressors NINJA and TOPLESS to JAZ‐bound transcription factors, and contains a highly conserved TIF[F/Y]XG motif that defines the larger family of TIFY proteins to which JAZs belong. Here, we report that diverse plant species contain genes encoding putative non‐TIFY JAZ proteins, including a previously unrecognized JAZ repressor in Arabidopsis (JAZ13, encoded by At3g22275). JAZ13 is most closely related to JAZ8 and includes divergent EAR, TIFY/ZIM, and Jas motifs. Unlike JAZ8, however, JAZ13 contains a Ser‐rich C‐terminal tail that is a site for phosphorylation. Overexpression of JAZ13 resulted in reduced sensitivity to JA, attenuation of wound‐induced expression of JA‐response genes, and decreased resistance to insect herbivory. JAZ13 interacts with the bHLH transcription factor MYC2 and the co‐repressor TOPLESS but, consistent with the absence of a TIFY motif, neither NINJA nor other JAZs. Analysis of single and higher‐order T‐DNA insertion jaz null mutants provided further evidence that JAZ13 is a repressor JA signaling. Our results demonstrate that proteins outside the TIFY family are functional JAZ repressors and further suggest that this expansion of the JAZ family allows fine‐tuning of JA‐mediated transcriptional responses.},
doi = {10.1111/tpj.12841},
journal = {The Plant Journal},
number = 4,
volume = 82,
place = {United Kingdom},
year = {Sat May 09 00:00:00 EDT 2015},
month = {Sat May 09 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.1111/tpj.12841

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