Polygold‐FISH for signal amplification of metallo‐labeled microbial cells
Abstract
An improved in situ hybridization approach (Polygold‐FISH) using biotinylated probes targeting multiple locations of the 16 S ribosomal subunit, followed by fluoronanogold‐streptavidin labeling and autometallographic enhancement of nanogold particles was developed as a means of signal amplification of metallo‐labeled cells, without the need for Catalyzed Reporter Deposition (CARD). Bacterial cells were readily detected based on their gold‐particle signal using scanning‐electron microscopy and energy‐dispersive X‐ray spectroscopy when contrasted with controls or cells hybridized with a single probe. Polygold‐FISH presents an alternative to CARD‐FISH, circumventing the need for aggressive oxidants, which is useful when products of microbial respiration such as those relevant at the microbe‐mineral interface could be altered during processing for visualization.
- Authors:
-
- Department of Civil and Environmental Engineering Colorado School of Mines Golden CO USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1400896
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Journal of Basic Microbiology
- Additional Journal Information:
- Journal Name: Journal of Basic Microbiology Journal Volume: 55 Journal Issue: 6; Journal ID: ISSN 0233-111X
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Germany
- Language:
- English
Citation Formats
Almstrand, Robert, Drennan, Dina M., and Sharp, Jonathan O. Polygold‐FISH for signal amplification of metallo‐labeled microbial cells. Germany: N. p., 2015.
Web. doi:10.1002/jobm.201400748.
Almstrand, Robert, Drennan, Dina M., & Sharp, Jonathan O. Polygold‐FISH for signal amplification of metallo‐labeled microbial cells. Germany. https://doi.org/10.1002/jobm.201400748
Almstrand, Robert, Drennan, Dina M., and Sharp, Jonathan O. Tue .
"Polygold‐FISH for signal amplification of metallo‐labeled microbial cells". Germany. https://doi.org/10.1002/jobm.201400748.
@article{osti_1400896,
title = {Polygold‐FISH for signal amplification of metallo‐labeled microbial cells},
author = {Almstrand, Robert and Drennan, Dina M. and Sharp, Jonathan O.},
abstractNote = {An improved in situ hybridization approach (Polygold‐FISH) using biotinylated probes targeting multiple locations of the 16 S ribosomal subunit, followed by fluoronanogold‐streptavidin labeling and autometallographic enhancement of nanogold particles was developed as a means of signal amplification of metallo‐labeled cells, without the need for Catalyzed Reporter Deposition (CARD). Bacterial cells were readily detected based on their gold‐particle signal using scanning‐electron microscopy and energy‐dispersive X‐ray spectroscopy when contrasted with controls or cells hybridized with a single probe. Polygold‐FISH presents an alternative to CARD‐FISH, circumventing the need for aggressive oxidants, which is useful when products of microbial respiration such as those relevant at the microbe‐mineral interface could be altered during processing for visualization.},
doi = {10.1002/jobm.201400748},
journal = {Journal of Basic Microbiology},
number = 6,
volume = 55,
place = {Germany},
year = {Tue Feb 03 00:00:00 EST 2015},
month = {Tue Feb 03 00:00:00 EST 2015}
}
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https://doi.org/10.1002/jobm.201400748
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