SurR is a master regulator of the primary electron flow pathways in the order Thermococcales

Lipscomb, Gina L.; Schut, Gerrit J.; Scott, Robert A.; Adams, Michael W. W.

doi:10.1111/mmi.13668

Title: SurR is a master regulator of the primary electron flow pathways in the order Thermococcales

Journal Article · 18 April 2017 · Molecular Microbiology

DOI: https://doi.org/10.1111/mmi.13668 · OSTI ID:1400621

Lipscomb, Gina L. ^[1]; Schut, Gerrit J. ^[1]; Scott, Robert A. ^[1];

^[1]

Department of Biochemistry and Molecular Biology University of Georgia Athens GA USA

Summary The sulfur response regulator, SurR, is among a handful of known redox‐active transcriptional regulators. First characterized from the hyperthermophile Pyrococcus furiosus , it is unique to the archaeal order Thermococcales. P. furiosus has two modes of electron disposal. Hydrogen gas is produced when the organism is grown in the absence of elemental sulfur (S ⁰ ) and H ₂ S is produced when grown in its presence. Switching between these metabolic modes requires a rapid transcriptional response and this is orchestrated by SurR. We show here that deletion of SurR causes severely impaired growth in the absence of S ⁰ since genes essential for H ₂ metabolism are no longer activated. Conversely, a strain containing a constitutively active SurR variant displays a growth phenotype in the presence of S ⁰ due to constitutive repression of S ⁰ ‐responsive genes. During a metabolic shift initiated by addition of S ⁰ to the growth medium, both strains demonstrate a de‐regulation of genes involved in the SurR regulon, including hydrogenase and related S ⁰ ‐responsive genes. These results demonstrate that SurR is a master regulator of electron flow within P. furiosus , likely affecting the pools of ferredoxin, NADPH and NADH, as well as influencing metabolic pathways and thiol/disulfide redox balance.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-08ER64690; FG05-95ER20175

OSTI ID:: 1400621

Journal Information:: Molecular Microbiology, Journal Name: Molecular Microbiology Journal Issue: 5 Vol. 104; ISSN 0950-382X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: FAO

Language:: English

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