Effect of in Vivo Deuteration on Structure of Switchgrass Lignin
Abstract
Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study, production of deuterated switchgrass was accomplished by growing the plants in 50% D2O under hydroponic conditions in a perfusion chamber. Cellulolytic enzyme lignin was isolated from deuterated switchgrass, characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) and compared with its protiated control sample to determine the effect of in vivo deuteration on the chemical structure of lignin. FTIR results showed that D2O can be taken up by the roots and transported to the leaves, and deuterium was subsequently incorporated into hydroxyl and alkyl groups in the plant and its lignin through photosynthesis. According to GPC results, deuterated lignin had slightly higher molecular weight, presumably due to isotope effects. 31P and heteronuclear single quantum coherence (HSQC) NMR results revealed that lignin in the deuterated biomass preserved its native physicochemical characteristics. Finally, the conserved characteristics of the deuterated lignin show its great potential applications for structural and dynamic studies of lignocellulose by techniques such as neutron scattering.
- Authors:
-
[2];
[2];
[2];
[2];
[3]
- Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1399953
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Sustainable Chemistry & Engineering
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 9; Journal ID: ISSN 2168-0485
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Cellulolytic enzyme lignin; Deuteration; In vivo; Neutron scattering; Switchgrass
Citation Formats
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., and Ragauskas, Arthur J. Effect of in Vivo Deuteration on Structure of Switchgrass Lignin. United States: N. p., 2017.
Web. doi:10.1021/acssuschemeng.7b01527.
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., & Ragauskas, Arthur J. Effect of in Vivo Deuteration on Structure of Switchgrass Lignin. United States. https://doi.org/10.1021/acssuschemeng.7b01527
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., and Ragauskas, Arthur J. Thu .
"Effect of in Vivo Deuteration on Structure of Switchgrass Lignin". United States. https://doi.org/10.1021/acssuschemeng.7b01527. https://www.osti.gov/servlets/purl/1399953.
@article{osti_1399953,
title = {Effect of in Vivo Deuteration on Structure of Switchgrass Lignin},
author = {Meng, Xianzhi and Evans, Barbara R. and Yoo, Chang Geun and Pu, Yunqiao Joseph and Davison, Brian H. and Ragauskas, Arthur J.},
abstractNote = {Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study, production of deuterated switchgrass was accomplished by growing the plants in 50% D2O under hydroponic conditions in a perfusion chamber. Cellulolytic enzyme lignin was isolated from deuterated switchgrass, characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) and compared with its protiated control sample to determine the effect of in vivo deuteration on the chemical structure of lignin. FTIR results showed that D2O can be taken up by the roots and transported to the leaves, and deuterium was subsequently incorporated into hydroxyl and alkyl groups in the plant and its lignin through photosynthesis. According to GPC results, deuterated lignin had slightly higher molecular weight, presumably due to isotope effects. 31P and heteronuclear single quantum coherence (HSQC) NMR results revealed that lignin in the deuterated biomass preserved its native physicochemical characteristics. Finally, the conserved characteristics of the deuterated lignin show its great potential applications for structural and dynamic studies of lignocellulose by techniques such as neutron scattering.},
doi = {10.1021/acssuschemeng.7b01527},
journal = {ACS Sustainable Chemistry & Engineering},
number = 9,
volume = 5,
place = {United States},
year = {Thu Jul 27 00:00:00 EDT 2017},
month = {Thu Jul 27 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 6 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
The Path Forward for Biofuels and Biomaterials
journal, January 2006
- Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
- Science, Vol. 311, Issue 5760, p. 484-489
Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose
journal, March 2012
- Zhao, Xuebing; Zhang, Lihua; Liu, Dehua
- Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 4
Characterization of the degree of polymerization of xylooligomers produced by flowthrough hydrolysis of pure xylan and corn stover with water
journal, September 2008
- Yang, Bin; Wyman, Charles E.
- Bioresource Technology, Vol. 99, Issue 13
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010
- Park, Sunkyu; Baker, John O.; Himmel, Michael E.
- Biotechnology for Biofuels, Vol. 3, Issue 1
Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis
journal, January 2010
- Arantes, Valdeir; Saddler, Jack N.
- Biotechnology for Biofuels, Vol. 3, Issue 1
The effect of fiber characteristics on hydrolysis and cellulase accessibility to softwood substrates
journal, November 1999
- Mooney, Caitriona A.; Mansfield, Shawn D.; Beatson, Rodger P.
- Enzyme and Microbial Technology, Vol. 25, Issue 8-9
How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012
- Ding, S. -Y.; Liu, Y. -S.; Zeng, Y.
- Science, Vol. 338, Issue 6110
Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014
- Meng, Xianzhi; Ragauskas, Arthur Jonas
- Current Opinion in Biotechnology, Vol. 27
Neutron Technologies for Bioenergy Research
journal, August 2012
- Langan, Paul; Evans, Barbara R.; Foston, Marcus
- Industrial Biotechnology, Vol. 8, Issue 4
Theory, practice and prospects of X-ray and neutron scattering for lignocellulosic biomass characterization: towards understanding biomass pretreatment
journal, January 2015
- Cheng, Gang; Zhang, Xin; Simmons, Blake
- Energy & Environmental Science, Vol. 8, Issue 2
Effect of D 2 O on Growth Properties and Chemical Structure of Annual Ryegrass ( Lolium multiflorum )
journal, March 2014
- Evans, Barbara R.; Bali, Garima; Reeves, David T.
- Journal of Agricultural and Food Chemistry, Vol. 62, Issue 12
Isotopic Composition of Cellulose from C3, C4, and CAM Plants Growing Near One Another
journal, May 1983
- Sternberg, L.; Deniro, M. J.
- Science, Vol. 220, Issue 4600
Structure Determination of Partially Deuterated Carotenoids from Intrinsically Labeled Vegetables by HPLC-MS and 1 H NMR
journal, February 2005
- Putzbach, Karsten; Krucker, Manfred; Albert, Klaus
- Journal of Agricultural and Food Chemistry, Vol. 53, Issue 3
The effect of deuteration on the structure of bacterial cellulose
journal, June 2013
- Bali, Garima; Foston, Marcus B.; O’Neill, Hugh M.
- Carbohydrate Research, Vol. 374
Production of deuterated switchgrass by hydroponic cultivation
journal, April 2015
- Evans, Barbara R.; Bali, Garima; Foston, Marcus
- Planta, Vol. 242, Issue 1
Continuous 13 C and 15 N Labeling of Tree Litter using a Climate-Controlled Chamber
journal, October 2015
- Bernard, Michael J.; Pitz, Scott L.; Chang, Chih-Han
- Communications in Soil Science and Plant Analysis, Vol. 46, Issue 21
Evidence for a novel biosynthetic pathway that regulates the ratio of syringyl to guaiacyl residues in lignin in the differentiating xylem of Magnolia kobus DC
journal, February 1999
- Chen, Fang; Yasuda, Seiich; Fukushima, Kazuhiko
- Planta, Vol. 207, Issue 4
Lignification in poplar plantlets fed with deuterium-labelled lignin precursors
journal, September 2004
- Rolando, Christian; Daubresse, Nicolas; Pollet, Brigitte
- Comptes Rendus Biologies, Vol. 327, Issue 9-10
Dietary Lignins Are Precursors of Mammalian Lignans in Rats
journal, January 2004
- Begum, Aynun Nahar; Nicolle, Catherine; Mila, Isabelle
- The Journal of Nutrition, Vol. 134, Issue 1
Pyrolytic reactivities of deuterated β-ether-type lignin model dimers
journal, March 2015
- Watanabe, Toshihiro; Kawamoto, Haruo; Saka, Shiro
- Journal of Analytical and Applied Pyrolysis, Vol. 112
Deuterium incorporation in biomass cell wall components by NMR analysis
journal, January 2012
- Foston, Marcus B.; McGaughey, Joseph; O'Neill, Hugh
- The Analyst, Vol. 137, Issue 5
Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells
journal, September 2009
- Takahashi, Hideo; Shimada, Ichio
- Journal of Biomolecular NMR, Vol. 46, Issue 1
Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
journal, April 2014
- de Ghellinck, Alexis; Schaller, Hubert; Laux, Valérie
- PLoS ONE, Vol. 9, Issue 4
An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock
journal, December 2016
- Meng, Xianzhi; Pu, Yunqiao; Yoo, Chang Geun
- ChemSusChem, Vol. 10, Issue 1
Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5
journal, January 2010
- Kim, Hoon; Ralph, John
- Org. Biomol. Chem., Vol. 8, Issue 3
Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d 6
journal, March 2008
- Kim, Hoon; Ralph, John; Akiyama, Takuya
- BioEnergy Research, Vol. 1, Issue 1, p. 56-66
Structural Characterization and Comparison of Switchgrass Ball-milled Lignin Before and After Dilute Acid Pretreatment
journal, August 2009
- Samuel, Reichel; Pu, Yunqiao; Raman, Babu
- Applied Biochemistry and Biotechnology, Vol. 162, Issue 1
Physicochemical Structural Changes of Poplar and Switchgrass during Biomass Pretreatment and Enzymatic Hydrolysis
journal, July 2016
- Meng, Xianzhi; Sun, Qining; Kosa, Matyas
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 9
Role of Functional Groups in Lignin Inhibition of Enzymatic Hydrolysis of Cellulose to Glucose
journal, March 2008
- Pan, Xuejun
- Journal of Biobased Materials and Bioenergy, Vol. 2, Issue 1
Application of quantitative 31P NMR in biomass lignin and biofuel precursors characterization
journal, January 2011
- Pu, Yunqiao; Cao, Shilin; Ragauskas, Arthur J.
- Energy & Environmental Science, Vol. 4, Issue 9
Differences in the chemical structure of the lignins from sugarcane bagasse and straw
journal, October 2015
- del Río, José C.; Lino, Alessandro G.; Colodette, Jorge L.
- Biomass and Bioenergy, Vol. 81
Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods
journal, December 2011
- del Río, José C.; Rencoret, Jorge; Prinsen, Pepijn
- Journal of Agricultural and Food Chemistry, Vol. 60, Issue 23
Tricin, a Flavonoid Monomer in Monocot Lignification
journal, February 2015
- Lan, Wu; Lu, Fachuang; Regner, Matthew
- Plant Physiology, Vol. 167, Issue 4
Application of new expansion pretreatment method on agricultural waste. Part I: Influence of pretreatment on the properties of lignin
journal, October 2013
- Wu, Miao; Pang, Jinhui; Lu, Fachuang
- Industrial Crops and Products, Vol. 50
Works referencing / citing this record:
Diol pretreatment to fractionate a reactive lignin in lignocellulosic biomass biorefineries
journal, January 2019
- Dong, Chengyu; Meng, Xianzhi; Yeung, Chi Shun
- Green Chemistry, Vol. 21, Issue 10
Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass
journal, September 2018
- Bhagia, Samarthya; Meng, Xianzhi; Evans, Barbara R.
- Scientific Reports, Vol. 8, Issue 1