Effect of in Vivo Deuteration on Structure of Switchgrass Lignin
Abstract
Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study, production of deuterated switchgrass was accomplished by growing the plants in 50% D2O under hydroponic conditions in a perfusion chamber. Cellulolytic enzyme lignin was isolated from deuterated switchgrass, characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) and compared with its protiated control sample to determine the effect of in vivo deuteration on the chemical structure of lignin. FTIR results showed that D2O can be taken up by the roots and transported to the leaves, and deuterium was subsequently incorporated into hydroxyl and alkyl groups in the plant and its lignin through photosynthesis. According to GPC results, deuterated lignin had slightly higher molecular weight, presumably due to isotope effects. 31P and heteronuclear single quantum coherence (HSQC) NMR results revealed that lignin in the deuterated biomass preserved its native physicochemical characteristics. Finally, the conserved characteristics of the deuterated lignin show its great potential applications for structural and dynamic studies of lignocellulose by techniques such as neutron scattering.
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1399953
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Sustainable Chemistry & Engineering
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 9; Journal ID: ISSN 2168-0485
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Cellulolytic enzyme lignin; Deuteration; In vivo; Neutron scattering; Switchgrass
Citation Formats
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., and Ragauskas, Arthur J. Effect of in Vivo Deuteration on Structure of Switchgrass Lignin. United States: N. p., 2017.
Web. doi:10.1021/acssuschemeng.7b01527.
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., & Ragauskas, Arthur J. Effect of in Vivo Deuteration on Structure of Switchgrass Lignin. United States. https://doi.org/10.1021/acssuschemeng.7b01527
Meng, Xianzhi, Evans, Barbara R., Yoo, Chang Geun, Pu, Yunqiao Joseph, Davison, Brian H., and Ragauskas, Arthur J. Thu .
"Effect of in Vivo Deuteration on Structure of Switchgrass Lignin". United States. https://doi.org/10.1021/acssuschemeng.7b01527. https://www.osti.gov/servlets/purl/1399953.
@article{osti_1399953,
title = {Effect of in Vivo Deuteration on Structure of Switchgrass Lignin},
author = {Meng, Xianzhi and Evans, Barbara R. and Yoo, Chang Geun and Pu, Yunqiao Joseph and Davison, Brian H. and Ragauskas, Arthur J.},
abstractNote = {Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study, production of deuterated switchgrass was accomplished by growing the plants in 50% D2O under hydroponic conditions in a perfusion chamber. Cellulolytic enzyme lignin was isolated from deuterated switchgrass, characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) and compared with its protiated control sample to determine the effect of in vivo deuteration on the chemical structure of lignin. FTIR results showed that D2O can be taken up by the roots and transported to the leaves, and deuterium was subsequently incorporated into hydroxyl and alkyl groups in the plant and its lignin through photosynthesis. According to GPC results, deuterated lignin had slightly higher molecular weight, presumably due to isotope effects. 31P and heteronuclear single quantum coherence (HSQC) NMR results revealed that lignin in the deuterated biomass preserved its native physicochemical characteristics. Finally, the conserved characteristics of the deuterated lignin show its great potential applications for structural and dynamic studies of lignocellulose by techniques such as neutron scattering.},
doi = {10.1021/acssuschemeng.7b01527},
journal = {ACS Sustainable Chemistry & Engineering},
number = 9,
volume = 5,
place = {United States},
year = {Thu Jul 27 00:00:00 EDT 2017},
month = {Thu Jul 27 00:00:00 EDT 2017}
}
Web of Science
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