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Title: A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses

Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.
Authors:
 [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biotechnology and Bioengineering
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Systems Biology
Publication Date:
Report Number(s):
SAND2016-11988J
Journal ID: ISSN 2045-2322; srep44778
Grant/Contract Number:
AC04-94AL85000
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; biological techniques; biotechnology; viral infection
OSTI Identifier:
1399884

Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., and Meagher, Robert J.. A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. United States: N. p., Web. doi:10.1038/srep44778.
Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., & Meagher, Robert J.. A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. United States. doi:10.1038/srep44778.
Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., and Meagher, Robert J.. 2017. "A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses". United States. doi:10.1038/srep44778. https://www.osti.gov/servlets/purl/1399884.
@article{osti_1399884,
title = {A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses},
author = {Priye, Aashish and Bird, Sara W. and Light, Yooli K. and Ball, Cameron S. and Negrete, Oscar A. and Meagher, Robert J.},
abstractNote = {Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.},
doi = {10.1038/srep44778},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {2017},
month = {3}
}

Works referenced in this record:

PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
journal, June 2004

Mobile Phone Based Clinical Microscopy for Global Health Applications
journal, July 2009