A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
Abstract
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.
- Authors:
-
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Biotechnology and Bioengineering
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Dept. of Systems Biology
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
- OSTI Identifier:
- 1399884
- Report Number(s):
- SAND2016-11988J
Journal ID: ISSN 2045-2322; srep44778
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; biological techniques; biotechnology; viral infection
Citation Formats
Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., and Meagher, Robert J. A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. United States: N. p., 2017.
Web. doi:10.1038/srep44778.
Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., & Meagher, Robert J. A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. United States. https://doi.org/10.1038/srep44778
Priye, Aashish, Bird, Sara W., Light, Yooli K., Ball, Cameron S., Negrete, Oscar A., and Meagher, Robert J. Mon .
"A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses". United States. https://doi.org/10.1038/srep44778. https://www.osti.gov/servlets/purl/1399884.
@article{osti_1399884,
title = {A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses},
author = {Priye, Aashish and Bird, Sara W. and Light, Yooli K. and Ball, Cameron S. and Negrete, Oscar A. and Meagher, Robert J.},
abstractNote = {Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. We demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5 V USB source such as a USB power bank or solar panel. The smartphone employs a novel algorithm utilizing chromaticity to analyze fluorescence signals, which improves the discrimination of positive/negative signals by 5-fold when compared to detection with traditional RGB intensity sensors or the naked eye. The ability to detect ZIKV directly from crude human sample matrices (blood, urine, and saliva) demonstrates our device’s utility for widespread clinical deployment. Altogether, these advances enable our system to host the key components necessary to expand the use of nucleic acid amplification-based detection assays towards point-of-care settings where they are needed most.},
doi = {10.1038/srep44778},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {Mon Mar 20 00:00:00 EDT 2017},
month = {Mon Mar 20 00:00:00 EDT 2017}
}
Web of Science
Works referenced in this record:
The Zika Virus and Pregnancy
journal, July 2016
- Fiorentino, D. G.; Montero, F. J.
- Current Obstetrics and Gynecology Reports, Vol. 5, Issue 3
Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry
journal, December 2016
- Tian, Bo; Qiu, Zhen; Ma, Jing
- Biosensors and Bioelectronics, Vol. 86
Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study
journal, April 2016
- Cao-Lormeau, Van-Mai; Blake, Alexandre; Mons, Sandrine
- The Lancet, Vol. 387, Issue 10027
Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation
journal, November 2001
- Mori, Yasuyoshi; Nagamine, Kentaro; Tomita, Norihiro
- Biochemical and Biophysical Research Communications, Vol. 289, Issue 1
The molecular basis of variation in human color vision: Variation in human color vision
journal, February 2005
- Deeb, Ss
- Clinical Genetics, Vol. 67, Issue 5
Imaging and Sizing of Single DNA Molecules on a Mobile Phone
journal, November 2014
- Wei, Qingshan; Luo, Wei; Chiang, Samuel
- ACS Nano, Vol. 8, Issue 12
Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
journal, January 2016
- Wheeler, Sarah S.; Ball, Cameron S.; Langevin, Stanley A.
- PLOS ONE, Vol. 11, Issue 1
Smartphone quantifies Salmonella from paper microfluidics
journal, January 2013
- Park, Tu San; Li, Wenyue; McCracken, Katherine E.
- Lab on a Chip, Vol. 13, Issue 24
Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1
journal, February 2012
- Curtis, Kelly A.; Rudolph, Donna L.; Nejad, Irene
- PLoS ONE, Vol. 7, Issue 2
Instrument-Free Point-of-Care Molecular Detection of Zika Virus
journal, June 2016
- Song, Jinzhao; Mauk, Michael G.; Hackett, Brent A.
- Analytical Chemistry, Vol. 88, Issue 14
High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners
journal, June 2011
- Guo, Wanhua; Cao, Lin; Jia, Zhijun
- Protein Expression and Purification, Vol. 77, Issue 2
Low-Cost Mobile Phone Microscopy with a Reversed Mobile Phone Camera Lens
journal, May 2014
- Switz, Neil A.; D'Ambrosio, Michael V.; Fletcher, Daniel A.
- PLoS ONE, Vol. 9, Issue 5
Loop-mediated isothermal amplification of DNA
journal, June 2000
- Notomi, T.
- Nucleic Acids Research, Vol. 28, Issue 12
Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
journal, February 2016
- Añez, Germán; Heisey, Daniel A. R.; Chancey, Caren
- PLOS Neglected Tropical Diseases, Vol. 10, Issue 2
Smartphone-based detection of unlabeled DNA via electrochemical dissolution
journal, January 2013
- Huang, Yu-Wen; Ugaz, Victor M.
- The Analyst, Vol. 138, Issue 9
Rapid Detection Strategies for the Global Threat of Zika Virus: Current State, New Hypotheses, and Limitations
journal, October 2016
- Shukla, Shruti; Hong, Sung-Yong; Chung, Soo Hyun
- Frontiers in Microbiology, Vol. 7
Commercialization of microfluidic point-of-care diagnostic devices
journal, January 2012
- Chin, Curtis D.; Linder, Vincent; Sia, Samuel K.
- Lab on a Chip, Vol. 12, Issue 12
Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus
journal, September 2015
- Dauner, Allison L.; Mitra, Indrani; Gilliland, Theron
- Diagnostic Microbiology and Infectious Disease, Vol. 83, Issue 1
Rapid and Real-Time Detection of Chikungunya Virus by Reverse Transcription Loop-Mediated Isothermal Amplification Assay
journal, November 2006
- Parida, M. M.; Santhosh, S. R.; Dash, P. K.
- Journal of Clinical Microbiology, Vol. 45, Issue 2
Mobile Phone Based Clinical Microscopy for Global Health Applications
journal, July 2009
- Breslauer, David N.; Maamari, Robi N.; Switz, Neil A.
- PLoS ONE, Vol. 4, Issue 7, Article No. e6320
Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses
journal, March 2016
- Ball, Cameron S.; Light, Yooli K.; Koh, Chung-Yan
- Analytical Chemistry, Vol. 88, Issue 7
Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
journal, May 2007
- Klungthong, C.; Gibbons, R. V.; Thaisomboonsuk, B.
- Journal of Clinical Microbiology, Vol. 45, Issue 8
Paper microfluidic extraction and direct smartphone-based identification of pathogenic nucleic acids from field and clinical samples
journal, January 2014
- Fronczek, Christopher F.; Park, Tu San; Harshman, Dustin K.
- RSC Advances, Vol. 4, Issue 22
Age-specific contacts and travel patterns in the spatial spread of 2009 H1N1 influenza pandemic
journal, April 2013
- Apolloni, Andrea; Poletto, Chiara; Colizza, Vittoria
- BMC Infectious Diseases, Vol. 13, Issue 1
Loop-Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M. avium, and M. intracellulare in Sputum Samples
journal, June 2003
- Iwamoto, T.; Sonobe, T.; Hayashi, K.
- Journal of Clinical Microbiology, Vol. 41, Issue 6
Lensfree microscopy on a cellphone
journal, January 2010
- Tseng, Derek; Mudanyali, Onur; Oztoprak, Cetin
- Lab on a Chip, Vol. 10, Issue 14
Direct Blood Dry LAMP: A Rapid, Stable, and Easy Diagnostic Tool for Human African Trypanosomiasis
journal, March 2015
- Hayashida, Kyoko; Kajino, Kiichi; Hachaambwa, Lottie
- PLOS Neglected Tropical Diseases, Vol. 9, Issue 3
Molecular genetics of inherited variation in human color vision
journal, April 1986
- Nathans, J.; Piantanida, T.; Eddy, R.
- Science, Vol. 232, Issue 4747
Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
journal, January 2015
- Notomi, Tsugunori; Mori, Yasuyoshi; Tomita, Norihiro
- Journal of Microbiology, Vol. 53, Issue 1
Label-free biodetection using a smartphone
journal, January 2013
- Gallegos, Dustin; Long, Kenneth D.; Yu, Hojeong
- Lab on a Chip, Vol. 13, Issue 11
A Field-Tailored Reverse Transcription Loop-Mediated Isothermal Assay for High Sensitivity Detection of Plasmodium falciparum Infections
journal, November 2016
- Kemleu, Sylvie; Guelig, Dylan; Eboumbou Moukoko, Carole
- PLOS ONE, Vol. 11, Issue 11
Smartphone based health accessory for colorimetric detection of biomarkers in sweat and saliva
journal, January 2013
- Oncescu, Vlad; O'Dell, Dakota; Erickson, David
- Lab on a Chip, Vol. 13, Issue 16
Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification
journal, September 2015
- Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong
- PLOS ONE, Vol. 10, Issue 9
Zika fever and congenital Zika syndrome: An unexpected emerging arboviral disease
journal, May 2016
- Chan, Jasper F. W.; Choi, Garnet K. Y.; Yip, Cyril C. Y.
- Journal of Infection, Vol. 72, Issue 5
Point-of-care colorimetric detection with a smartphone
journal, January 2012
- Shen, Li; Hagen, Joshua A.; Papautsky, Ian
- Lab on a Chip, Vol. 12, Issue 21
Smartphone Fluorescence Spectroscopy
journal, August 2014
- Yu, Hojeong; Tan, Yafang; Cunningham, Brian T.
- Analytical Chemistry, Vol. 86, Issue 17
Zika virus — reigniting the TORCH
journal, August 2016
- Coyne, Carolyn B.; Lazear, Helen M.
- Nature Reviews Microbiology, Vol. 14, Issue 11
Solar thermal polymerase chain reaction for smartphone-assisted molecular diagnostics
journal, February 2014
- Jiang, Li; Mancuso, Matthew; Lu, Zhengda
- Scientific Reports, Vol. 4, Issue 1
Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification
journal, August 2013
- Teoh, Boon-Teong; Sam, Sing-Sin; Tan, Kim-Kee
- BMC Infectious Diseases, Vol. 13, Issue 1
Lab-on-a-Drone: Toward Pinpoint Deployment of Smartphone-Enabled Nucleic Acid-Based Diagnostics for Mobile Health Care
journal, April 2016
- Priye, Aashish; Wong, Season; Bi, Yuanpeng
- Analytical Chemistry, Vol. 88, Issue 9
Quantitative Imaging with a Mobile Phone Microscope
journal, May 2014
- Skandarajah, Arunan; Reber, Clay D.; Switz, Neil A.
- PLoS ONE, Vol. 9, Issue 5
Zika Virus
journal, June 2017
- Relich, Ryan F.; Loeffelholz, Michael
- Clinics in Laboratory Medicine, Vol. 37, Issue 2
Development of a loop-mediated isothermal amplification method for rapid detection of caprine arthritis-encephalitis virus proviral DNA
journal, May 2012
- Huang, Jinhai; Sun, Yuehui; Liu, Yebing
- Archives of Virology, Vol. 157, Issue 8
Development and evaluation of real-time loop-mediated isothermal amplification for hepatitis B virus DNA quantification: A new tool for HBV management
journal, April 2008
- Cai, Ting; Lou, GuoQiang; Yang, Jin
- Journal of Clinical Virology, Vol. 41, Issue 4
Multiplex loop-mediated isothermal amplification (M-LAMP) assay for the detection of influenza A/H1, A/H3 and influenza B can provide a specimen-to-result diagnosis in 40min with single genome copy sensitivity
journal, September 2013
- Mahony, James; Chong, Sylvia; Bulir, David
- Journal of Clinical Virology, Vol. 58, Issue 1
Neuraminidase inhibitors for treatment of human and avian strain influenza: A comparative modeling study
journal, January 2011
- Dobrovolny, Hana M.; Gieschke, Ronald; Davies, Brian E.
- Journal of Theoretical Biology, Vol. 269, Issue 1
Rapid detection and typing of influenza A and B by loop-mediated isothermal amplification: Comparison with immunochromatography and virus isolation
journal, August 2006
- Ito, Masahiro; Watanabe, Masahiro; Nakagawa, Naoko
- Journal of Virological Methods, Vol. 135, Issue 2
Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification method
journal, May 2007
- Imai, Masaki; Ninomiya, Ai; Minekawa, Harumi
- Journal of Virological Methods, Vol. 141, Issue 2
Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection
journal, August 2014
- Nakauchi, Mina; Takayama, Ikuyo; Takahashi, Hitoshi
- Journal of Virological Methods, Vol. 204
Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection
journal, November 2006
- Imai, Masaki; Ninomiya, Ai; Minekawa, Harumi
- Vaccine, Vol. 24, Issue 44-46
ZIKV actively induces calcification in the fetal brain
journal, March 2021
- Clements, Rebecca L.; Jurado, Kellie Ann
- Nature Microbiology, Vol. 6, Issue 4
Rapid acquisition of polymorphic virulence markers during adaptation of highly pathogenic avian influenza H5N8 virus in the mouse
journal, January 2017
- Choi, Won-Suk; Baek, Yun Hee; Kwon, Jin Jung
- Scientific Reports, Vol. 7, Issue 1
Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus
journal, March 2017
- Chi, Ying; Ge, Yiyue; Zhao, Kangchen
- Scientific Reports, Vol. 7, Issue 1
Sample preparation: a challenge in the development of point-of-care nucleic acid-based assays for resource-limited settings
journal, January 2007
- Dineva, Magda Anastassova; Mahilum-Tapay, Lourdes; Lee, Helen
- The Analyst, Vol. 132, Issue 12
Rapid-Test Sensitivity for Novel Swine-Origin Influenza A (H1N1) Virus in Humans
journal, August 2009
- Faix, Dennis J.; Sherman, Sterling S.; Waterman, Steven H.
- New England Journal of Medicine, Vol. 361, Issue 7
Rapid Antigen Tests for Diagnosis of Pandemic (Swine) Influenza A/H1N1
journal, October 2009
- Vasoo, Shawn; Stevens, Jane; Singh, Kamaljit
- Clinical Infectious Diseases, Vol. 49, Issue 7
Diagnosis of 2009 Pandemic Influenza A (pH1N1) and Seasonal Influenza Using Rapid Influenza Antigen Tests, San Antonio, Texas, April–June 2009
journal, January 2011
- Lucas, Pauline M.; Morgan, Oliver W.; Gibbons, Thomas F.
- Clinical Infectious Diseases, Vol. 52, Issue suppl_1
Visual detection of human infection with influenza A (H7N9) virus by subtype-specific reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye
journal, August 2013
- Nie, K.; Zhao, X.; Ding, X.
- Clinical Microbiology and Infection, Vol. 19, Issue 8
Rapid Detection of Tembusu Virus by Reverse-Transcription, Loop-mediated Isothermal Amplification (RT-LAMP): Rapid Detection of Tembusu Virus by RT-LAMP
journal, September 2011
- Tang, Y.; Diao, Y.; Yu, C.
- Transboundary and Emerging Diseases, Vol. 59, Issue 3
Detection of Respiratory Viruses by Molecular Methods
journal, October 2008
- Mahony, James B.
- Clinical Microbiology Reviews, Vol. 21, Issue 4
Point-of-Care Testing for Infectious Diseases: Past, Present, and Future
journal, August 2017
- Kozel, Thomas R.; Burnham-Marusich, Amanda R.
- Journal of Clinical Microbiology, Vol. 55, Issue 8
Sensitive and Rapid Detection of Herpes Simplex Virus and Varicella-Zoster Virus DNA by Loop-Mediated Isothermal Amplification
journal, July 2005
- Kaneko, Hisatoshi; Iida, Tomohiro; Aoki, Koki
- Journal of Clinical Microbiology, Vol. 43, Issue 7
Reverse-transcription, loop-mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
journal, September 2015
- Luo, Sisi; Xie, Zhixun; Xie, Liji
- Virology Journal, Vol. 12, Issue 1
Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for H7N7 Equine Influenza Virus
journal, January 2012
- Nemoto, Manabu; Yamanaka, Takashi; Bannai, Hiroshi
- Journal of Veterinary Medical Science, Vol. 74, Issue 7
Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
journal, August 2010
- Chen, Mark I.; Barr, Ian G.; Koh, Gerald C. H.
- PLoS ONE, Vol. 5, Issue 8
Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
journal, February 2015
- Tanner, Nathan A.; Zhang, Yinhua; Evans, Thomas C.
- BioTechniques, Vol. 58, Issue 2
Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
journal, June 2007
- Jayawardena, Shanthi; Cheung, Chung Y.; Barr, Ian
- Emerging Infectious Diseases, Vol. 13, Issue 6
Highly Pathogenic Avian Influenza Virus (H5N1) in Domestic Poultry and Relationship with Migratory Birds, South Korea
journal, March 2008
- Lee, Youn-Jeong; Choi, Young-Ki; Kim, Yong-Joo
- Emerging Infectious Diseases, Vol. 14, Issue 3
Tools to Detect Influenza Virus
journal, January 2013
- Kim, Dae-Ki; Poudel, Barun
- Yonsei Medical Journal, Vol. 54, Issue 3
Zika Virus
journal, August 2016
- Shastry, Siri; Koenig, Kristi L.; Hirshon, Jon Mark
- Emergency Medicine Clinics of North America, Vol. 34, Issue 3
Zika virus — reigniting the TORCH
text, January 2016
- B., Coyne, Carolyn; M., Lazear, Helen
- The University of North Carolina at Chapel Hill University Libraries
Smartphone fluorescence spectroscopy
conference, March 2015
- Yu, Hojoeng; Tan, Yafang; Cunningham, Brian T.
- SPIE BiOS, SPIE Proceedings
Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation
journal, November 2001
- Mori, Yasuyoshi; Nagamine, Kentaro; Tomita, Norihiro
- Biochemical and Biophysical Research Communications, Vol. 289, Issue 1
Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry
journal, December 2016
- Tian, Bo; Qiu, Zhen; Ma, Jing
- Biosensors and Bioelectronics, Vol. 86
Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus
journal, September 2015
- Dauner, Allison L.; Mitra, Indrani; Gilliland, Theron
- Diagnostic Microbiology and Infectious Disease, Vol. 83, Issue 1
Outbreak of Kyasanur Forest disease (monkey fever) in Sindhudurg, Maharashtra State, India, 2016
journal, June 2016
- Awate, P.; Yadav, P.; Patil, D.
- Journal of Infection, Vol. 72, Issue 6
High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners
journal, June 2011
- Guo, Wanhua; Cao, Lin; Jia, Zhijun
- Protein Expression and Purification, Vol. 77, Issue 2
PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
journal, June 2004
- Yang, Samuel; Rothman, Richard E.
- The Lancet Infectious Diseases, Vol. 4, Issue 6, p. 337-348
Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses
journal, March 2016
- Ball, Cameron S.; Light, Yooli K.; Koh, Chung-Yan
- Analytical Chemistry, Vol. 88, Issue 7
Correction to “Instrument-Free Point-of-Care Molecular Detection of Zika Virus”
journal, October 2018
- Song, Jinzhao; Mauk, Michael G.; Hackett, Brent A.
- Analytical Chemistry, Vol. 90, Issue 22
Imaging and Sizing of Single DNA Molecules on a Mobile Phone
journal, November 2014
- Wei, Qingshan; Luo, Wei; Chiang, Samuel
- ACS Nano, Vol. 8, Issue 12
Commercialization of microfluidic point-of-care diagnostic devices
journal, January 2012
- Chin, Curtis D.; Linder, Vincent; Sia, Samuel K.
- Lab on a Chip, Vol. 12, Issue 12
Smartphone-based detection of unlabeled DNA via electrochemical dissolution
journal, January 2013
- Huang, Yu-Wen; Ugaz, Victor M.
- The Analyst, Vol. 138, Issue 9
Label-free biodetection using a smartphone
journal, January 2013
- Gallegos, Dustin; Long, Kenneth D.; Yu, Hojeong
- Lab on a Chip, Vol. 13, Issue 11
Smartphone based health accessory for colorimetric detection of biomarkers in sweat and saliva
journal, January 2013
- Oncescu, Vlad; O'Dell, Dakota; Erickson, David
- Lab on a Chip, Vol. 13, Issue 16
Cholesterol testing on a smartphone
journal, January 2014
- Oncescu, Vlad; Mancuso, Matthew; Erickson, David
- Lab Chip, Vol. 14, Issue 4
A smartphone platform for the quantification of vitamin D levels
journal, January 2014
- Lee, Seoho; Oncescu, Vlad; Mancuso, Matt
- Lab Chip, Vol. 14, Issue 8
Loop-mediated isothermal amplification of DNA
journal, June 2000
- Notomi, T.
- Nucleic Acids Research, Vol. 28, Issue 12
Smartphone fluorescence spectroscopy
conference, March 2015
- Yu, Hojoeng; Tan, Yafang; Cunningham, Brian T.
- SPIE BiOS, SPIE Proceedings
Zika Virus
journal, March 2016
- Musso, Didier; Gubler, Duane J.
- Clinical Microbiology Reviews, Vol. 29, Issue 3
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay
journal, June 2005
- Parida, M.; Horioke, K.; Ishida, H.
- Journal of Clinical Microbiology, Vol. 43, Issue 6
Antiviral Resistance and Correlates of Virologic Failure in the first Cohort of HIV-Infected Children Gaining Access to Structured Antiretroviral Therapy in Lima, Peru: A Cross-Sectional Analysis
journal, January 2013
- Rath, Barbara A.; von Kleist, Max; Castillo, Maria E.
- BMC Infectious Diseases, Vol. 13, Issue 1
Direct Blood Dry LAMP: A Rapid, Stable, and Easy Diagnostic Tool for Human African Trypanosomiasis
journal, March 2015
- Hayashida, Kyoko; Kajino, Kiichi; Hachaambwa, Lottie
- PLOS Neglected Tropical Diseases, Vol. 9, Issue 3
Mobile Phone Based Clinical Microscopy for Global Health Applications
journal, July 2009
- Breslauer, David N.; Maamari, Robi N.; Switz, Neil A.
- PLoS ONE, Vol. 4, Issue 7, Article No. e6320
Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1
journal, February 2012
- Curtis, Kelly A.; Rudolph, Donna L.; Nejad, Irene
- PLoS ONE, Vol. 7, Issue 2
Quantitative Imaging with a Mobile Phone Microscope
journal, May 2014
- Skandarajah, Arunan; Reber, Clay D.; Switz, Neil A.
- PLoS ONE, Vol. 9, Issue 5
Colorimetric Detection of Dengue by Single Tube Reverse-Transcription-Loop-Mediated Isothermal Amplification
journal, September 2015
- Lau, Yee-Ling; Lai, Meng-Yee; Teoh, Boon-Teong
- PLOS ONE, Vol. 10, Issue 9
Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
journal, January 2016
- Wheeler, Sarah S.; Ball, Cameron S.; Langevin, Stanley A.
- PLOS ONE, Vol. 11, Issue 1
Zika virus — reigniting the TORCH
text, January 2016
- B., Coyne, Carolyn; M., Lazear, Helen
- The University of North Carolina at Chapel Hill University Libraries
Rapid Detection Strategies for the Global Threat of Zika Virus: Current State, New Hypotheses, and Limitations
journal, October 2016
- Shukla, Shruti; Hong, Sung-Yong; Chung, Soo Hyun
- Frontiers in Microbiology, Vol. 7
Works referencing / citing this record:
Biosensors for Personal Mobile Health: A System Architecture Perspective
journal, November 2019
- Arumugam, Siddarth; Colburn, David A. M.; Sia, Samuel K.
- Advanced Materials Technologies, Vol. 5, Issue 3
Prototypen-Entwicklung von Instrumenten für das chemische Laboratorium mithilfe von preiswerten Elektronikmodulen
journal, July 2018
- Urban, Pawel L.
- Angewandte Chemie, Vol. 130, Issue 34
Prototyping Instruments for the Chemical Laboratory Using Inexpensive Electronic Modules
journal, July 2018
- Urban, Pawel L.
- Angewandte Chemie International Edition, Vol. 57, Issue 34
Valve-Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection
journal, November 2018
- Jiang, Xiao; Loeb, Julia C.; Manzanas, Carlos
- Angewandte Chemie International Edition, Vol. 57, Issue 52
Detection of chikungunya virus‐specific IgM on laser‐cut paper‐based device using pseudo‐particles as capture antigen
journal, February 2019
- Theillet, Gerald; Grard, Gilda; Galla, Mathilde
- Journal of Medical Virology, Vol. 91, Issue 6
Dengue virus: a review on advances in detection and trends – from conventional methods to novel biosensors
journal, May 2019
- Eivazzadeh-Keihan, Reza; Pashazadeh-Panahi, Paria; Mahmoudi, Tohid
- Microchimica Acta, Vol. 186, Issue 6
Conductance quantization as a new selective sensing mechanism in dendritic point contacts
journal, February 2019
- Kamarchuk, Gennadii V.; Pospelov, Alexander P.; Savytskyi, Andriy V.
- SN Applied Sciences, Vol. 1, Issue 3
Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning
journal, July 2018
- Im, Hyungsoon; Pathania, Divya; McFarland, Philip J.
- Nature Biomedical Engineering, Vol. 2, Issue 9
Rapid Detection of Zika Virus in Urine Samples and Infected Mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification
journal, February 2018
- Lamb, Laura E.; Bartolone, Sarah N.; Tree, Maya O.
- Scientific Reports, Vol. 8, Issue 1
A sensitive electrochemical immunosensor for label-free detection of Zika-virus protein
journal, June 2018
- Kaushik, Ajeet; Yndart, Adriana; Kumar, Sanjeev
- Scientific Reports, Vol. 8, Issue 1
Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
journal, August 2018
- Kaarj, Kattika; Akarapipad, Patarajarin; Yoon, Jeong-Yeol
- Scientific Reports, Vol. 8, Issue 1
Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP)
journal, September 2018
- Li, Zhili; Cai, Yuejia; Liang, Guozhi
- Scientific Reports, Vol. 8, Issue 1
Development and Validation of Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for Rapid Detection of ZIKV in Mosquito Samples from Brazil
journal, March 2019
- Silva, Severino Jefferson Ribeiro da; Paiva, Marcelo Henrique Santos; Guedes, Duschinka Ribeiro Duarte
- Scientific Reports, Vol. 9, Issue 1
Interfacial Effect-Based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection
journal, July 2019
- Ulep, Tiffany-Heather; Day, Alexander S.; Sosnowski, Katelyn
- Scientific Reports, Vol. 9, Issue 1
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
journal, February 2020
- Jue, Erik; Witters, Daan; Ismagilov, Rustem F.
- Scientific Reports, Vol. 10, Issue 1
Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA
journal, January 2018
- Meagher, Robert J.; Priye, Aashish; Light, Yooli K.
- The Analyst, Vol. 143, Issue 8
Mobile diagnostics: next-generation technologies for in vitro diagnostics
journal, January 2018
- Shin, Joonchul; Chakravarty, Sudesna; Choi, Wooseok
- The Analyst, Vol. 143, Issue 7
Buffer-free integrative nanofluidic device for real-time continuous flow bioassays by ion concentration polarization
journal, January 2018
- Phan, Dinh-Tuan; Jin, Lin; Wustoni, Shofarul
- Lab on a Chip, Vol. 18, Issue 4
Barcoded point-of-care bioassays
journal, January 2019
- Yang, Mingzhu; Liu, Yong; Jiang, Xingyu
- Chemical Society Reviews, Vol. 48, Issue 3
Multiplex sample-to-answer detection of bacteria using a pipette-actuated capillary array comb with integrated DNA extraction, isothermal amplification, and smartphone detection
journal, January 2018
- Hui, Junhou; Gu, Yin; Zhu, Yuanshou
- Lab on a Chip, Vol. 18, Issue 18
Digital polymerase chain reaction technology – recent advances and future perspectives
journal, January 2018
- Sreejith, Kamalalayam Rajan; Ooi, Chin Hong; Jin, Jing
- Lab on a Chip, Vol. 18, Issue 24
Loop-mediated isothermal amplification (LAMP) – review and classification of methods for sequence-specific detection
journal, January 2020
- Becherer, Lisa; Borst, Nadine; Bakheit, Mohammed
- Analytical Methods, Vol. 12, Issue 6
Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors
journal, July 2018
- Yang, Jiancheng; Carey, Patrick; Ren, Fan
- Applied Physics Letters, Vol. 113, Issue 3
Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)
journal, January 2018
- Castro, Talita; Sabalza, Maite; Barber, Cheryl
- Journal of Oral Microbiology, Vol. 10, Issue 1
Innovative and New Approaches to Laboratory Diagnosis of Zika and Dengue: A Meeting Report
journal, December 2017
- Goncalves, Adriana; Peeling, Rosanna W.; Chu, May C.
- The Journal of Infectious Diseases, Vol. 217, Issue 7
Smartphone-based clinical diagnostics: towards democratization of evidence-based health care
journal, September 2018
- Hernández-Neuta, I.; Neumann, F.; Brightmeyer, J.
- Journal of Internal Medicine, Vol. 285, Issue 1
Mobile oral heath technologies based on saliva
journal, February 2018
- Meagher, Rj; Kousvelari, E.
- Oral Diseases, Vol. 24, Issue 1-2
Beyond Fever and Pain: Diagnostic Methods for Chikungunya Virus
journal, May 2019
- Natrajan, Muktha S.; Rojas, Alejandra; Waggoner, Jesse J.
- Journal of Clinical Microbiology, Vol. 57, Issue 6
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
journal, August 2019
- Ahn, Su Jeong; Baek, Yun Hee; Lloren, Khristine Kaith S.
- BMC Infectious Diseases, Vol. 19, Issue 1
Laboratory Diagnosis and Potential Application of Nucleic Acid Biosensor Approach for Early Detection of Dengue Virus Infections
journal, June 2018
- Izuan Abdul Rashid, Jahwarhar; Azah Yusof, Nor
- Biosciences, Biotechnology Research Asia, Vol. 15, Issue 2
Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva
journal, February 2018
- Sabalza, Maite; Yasmin, Rubina; Barber, Cheryl A.
- PLOS ONE, Vol. 13, Issue 2
A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus
journal, January 2019
- Takemura, Kenshin; Adegoke, Oluwasesan; Suzuki, Tetsuro
- PLOS ONE, Vol. 14, Issue 1
Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update
journal, January 2018
- Singh, Raj K.; Dhama, Kuldeep; Karthik, Kumaragurubaran
- Frontiers in Microbiology, Vol. 8
Advances in Directly Amplifying Nucleic Acids from Complex Samples
journal, September 2019
- Walker, Faye M.; Hsieh, Kuangwen
- Biosensors, Vol. 9, Issue 4
Smartphone Coupled with a Paper-Based Colorimetric Device for Sensitive and Portable Mercury Ion Sensing
journal, May 2019
- Firdaus, M. Lutfi; Aprian, Angga; Meileza, Nessi
- Chemosensors, Vol. 7, Issue 2
Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens
journal, February 2018
- Higgins, Owen; Clancy, Eoin; Cormican, Martin
- International Journal of Molecular Sciences, Vol. 19, Issue 2
A Nontoxic Battery with 3D-Printed Housing for On-Demand Operation of Microcontrollers in Microfluidic Sensors
journal, September 2019
- Sachsenheimer, Kai; Richter, Christiane; Helmer, Dorothea
- Micromachines, Vol. 10, Issue 9
Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection
journal, November 2018
- Jiang, Xiao; Loeb, Julia C.; Manzanas, Carlos
- Angewandte Chemie, Vol. 130, Issue 52
One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011
- Berry, Scott M.; Alarid, Elaine T.; Beebe, David J.
- Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753
DNA microarray analysis using a smartphone to detect the BRCA-1 gene
journal, January 2019
- Prasad, Alisha; Hasan, Syed Mohammad Abid; Grouchy, Steven
- The Analyst, Vol. 144, Issue 1
An inexpensive smartphone-based device for point-of-care ovulation testing
journal, January 2019
- Potluri, Vaishnavi; Kathiresan, Preethi Sangeetha; Kandula, Hemanth
- Lab on a Chip, Vol. 19, Issue 1
Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
journal, February 2018
- Velders, Aldrik H.; Schoen, Cor; Saggiomo, Vittorio
- BMC Research Notes, Vol. 11, Issue 1
2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics
journal, March 2020
- Nguyen, Trieu; Duong Bang, Dang; Wolff, Anders
- Micromachines, Vol. 11, Issue 3
Developments in Transduction, Connectivity and AI/Machine Learning for Point-of-Care Testing
journal, April 2019
- O’Sullivan, Shane; Ali, Zulfiqur; Jiang, Xiaoyi
- Sensors, Vol. 19, Issue 8
Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review
journal, December 2019
- Silva, Severino Jefferson Ribeiro da; Pardee, Keith; Pena, Lindomar
- Viruses, Vol. 12, Issue 1
Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2
text, January 2020
- Baek, Yun Hee; Um, Jihye; Antigua, Khristine Joy C.
- Taylor & Francis
Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
journal, September 2017
- Calvert, Amanda E.; Biggerstaff, Brad J.; Tanner, Nathan A.
- PLOS ONE, Vol. 12, Issue 9
Smartphone-based pathogen diagnosis in urinary sepsis patients
journal, October 2018
- Barnes, Lucien; Heithoff, Douglas M.; Mahan, Scott P.
- EBioMedicine, Vol. 36
A Rapid and Low-Cost Pathogen Detection Platform by Using a Molecular Agglutination Assay
journal, November 2018
- Wu, Tsung-Feng; Chen, Yu-Chen; Wang, Wei-Chung
- ACS Central Science, Vol. 4, Issue 11
Benchmarking Smartphone Fluorescence-Based Microscopy with DNA Origami Nanobeads: Reducing the Gap toward Single-Molecule Sensitivity
journal, January 2019
- Vietz, Carolin; Schütte, Max L.; Wei, Qingshan
- ACS Omega, Vol. 4, Issue 1
Design and clinical validation of a point-of-care device for the diagnosis of lymphoma via contrast-enhanced microholography and machine learning
journal, July 2018
- Im, Hyungsoon; Pathania, Divya; McFarland, Philip J.
- Nature Biomedical Engineering, Vol. 2, Issue 9
Rapid Detection of Zika Virus in Urine Samples and Infected Mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification
journal, February 2018
- Lamb, Laura E.; Bartolone, Sarah N.; Tree, Maya O.
- Scientific Reports, Vol. 8, Issue 1
Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips
journal, August 2018
- Kaarj, Kattika; Akarapipad, Patarajarin; Yoon, Jeong-Yeol
- Scientific Reports, Vol. 8, Issue 1
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
journal, February 2020
- Jue, Erik; Witters, Daan; Ismagilov, Rustem F.
- Scientific Reports, Vol. 10, Issue 1
Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)
journal, January 2018
- Castro, Talita; Sabalza, Maite; Barber, Cheryl
- Journal of Oral Microbiology, Vol. 10, Issue 1
Smartphone‐based clinical diagnostics and atrial fibrillation: the need to avoid being out of sight
journal, May 2019
- Leggio, M.; Villano, A.; Fusco, A.
- Journal of Internal Medicine, Vol. 286, Issue 3
Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform
journal, August 2019
- Ahn, Su Jeong; Baek, Yun Hee; Lloren, Khristine Kaith S.
- BMC Infectious Diseases, Vol. 19, Issue 1
Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
journal, September 2017
- Calvert, Amanda E.; Biggerstaff, Brad J.; Tanner, Nathan A.
- PLOS ONE, Vol. 12, Issue 9
Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva
journal, February 2018
- Sabalza, Maite; Yasmin, Rubina; Barber, Cheryl A.
- PLOS ONE, Vol. 13, Issue 2
A localized surface plasmon resonance-amplified immunofluorescence biosensor for ultrasensitive and rapid detection of nonstructural protein 1 of Zika virus
journal, January 2019
- Takemura, Kenshin; Adegoke, Oluwasesan; Suzuki, Tetsuro
- PLOS ONE, Vol. 14, Issue 1
Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update
journal, January 2018
- Singh, Raj K.; Dhama, Kuldeep; Karthik, Kumaragurubaran
- Frontiers in Microbiology, Vol. 8
“The Smartphone’s Guide to the Galaxy”: In Situ Analysis in Space
journal, October 2018
- Nelis, Joost; Elliott, Christopher; Campbell, Katrina
- Biosensors, Vol. 8, Issue 4
Advances in Directly Amplifying Nucleic Acids from Complex Samples
journal, September 2019
- Walker, Faye M.; Hsieh, Kuangwen
- Biosensors, Vol. 9, Issue 4
Smartphone Coupled with a Paper-Based Colorimetric Device for Sensitive and Portable Mercury Ion Sensing
journal, May 2019
- Firdaus, M. Lutfi; Aprian, Angga; Meileza, Nessi
- Chemosensors, Vol. 7, Issue 2
Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens
journal, February 2018
- Higgins, Owen; Clancy, Eoin; Cormican, Martin
- International Journal of Molecular Sciences, Vol. 19, Issue 2
A Nontoxic Battery with 3D-Printed Housing for On-Demand Operation of Microcontrollers in Microfluidic Sensors
journal, September 2019
- Sachsenheimer, Kai; Richter, Christiane; Helmer, Dorothea
- Micromachines, Vol. 10, Issue 9
Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review
journal, December 2019
- Silva, Severino Jefferson Ribeiro da; Pardee, Keith; Pena, Lindomar
- Viruses, Vol. 12, Issue 1
Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening
journal, January 2019
- Yin, Kun; Pandian, Vikram; Kadimisetty, Karteek
- Theranostics, Vol. 9, Issue 9