Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions
Abstract
Bioremediation at chlorinated solvent sites often leads to groundwater acidification due to electron donor fermentation and enhanced dechlorination activity. The microbial reductive dechlorination process is robust at circumneutral pH, but activity declines at groundwater pH values below 6.0. Consistent with this observation, the activity of tetrachloroethene (PCE) dechlorinating cultures declined at pH 6.0 and was not sustained in pH 5.5 medium, with one notable exception. Sulf urospirillum multivorans dechlorinated PCE to cis-1,2-dichloroethene (cDCE) in pH 5.5 medium and maintained this activity upon repeated transfers. Microcosms established with soil and aquifer materials from five distinct locations dechlorinated PCE-to-ethene at pH 5.5 and pH 7.2. Dechlorination to ethene was maintained following repeated transfers at pH 7.2, but no ethene was produced at pH 5.5, and only the transfer cultures derived from the Axton Cross Superfund (ACS) microcosms sustained PCE dechlorination to cDCE as a final product. 16S rRNA gene amplicon sequencing of pH 7.2 and pH 5.5 ACS enrichments revealed distinct microbial communities, with the dominant dechlorinator being Dehalococcoides in pH 7.2 and Sulf urospirillum in pH 5.5 cultures. PCE-to-trichloroethene- (TCE-) and PCE-to-cDCEdechlorinating isolates obtained from the ACS pH 5.5 enrichment shared 98.6%, and 98.5% 16S rRNA gene sequence similarities to Sulfmore »
- Authors:
-
[2];
[4]
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS)
- Tufts Univ., Medford, MA (United States). Dept. of Civil and Environmental Engineering
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Microbiology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS)
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, Dept. of Microbiology, Dept. of Biosystems Engineering and Soil Science; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, and joint Inst. for Biological Sciences (JIBS)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; USDoD
- OSTI Identifier:
- 1399396
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 51; Journal Issue: 15; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., and Löffler, Frank E. Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions. United States: N. p., 2017.
Web. doi:10.1021/acs.est.7b01510.
Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., & Löffler, Frank E. Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions. United States. https://doi.org/10.1021/acs.est.7b01510
Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., and Löffler, Frank E. Fri .
"Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions". United States. https://doi.org/10.1021/acs.est.7b01510. https://www.osti.gov/servlets/purl/1399396.
@article{osti_1399396,
title = {Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions},
author = {Yang, Yi and Cápiro, Natalie L. and Marcet, Tyler F. and Yan, Jun and Pennell, Kurt D. and Löffler, Frank E.},
abstractNote = {Bioremediation at chlorinated solvent sites often leads to groundwater acidification due to electron donor fermentation and enhanced dechlorination activity. The microbial reductive dechlorination process is robust at circumneutral pH, but activity declines at groundwater pH values below 6.0. Consistent with this observation, the activity of tetrachloroethene (PCE) dechlorinating cultures declined at pH 6.0 and was not sustained in pH 5.5 medium, with one notable exception. Sulf urospirillum multivorans dechlorinated PCE to cis-1,2-dichloroethene (cDCE) in pH 5.5 medium and maintained this activity upon repeated transfers. Microcosms established with soil and aquifer materials from five distinct locations dechlorinated PCE-to-ethene at pH 5.5 and pH 7.2. Dechlorination to ethene was maintained following repeated transfers at pH 7.2, but no ethene was produced at pH 5.5, and only the transfer cultures derived from the Axton Cross Superfund (ACS) microcosms sustained PCE dechlorination to cDCE as a final product. 16S rRNA gene amplicon sequencing of pH 7.2 and pH 5.5 ACS enrichments revealed distinct microbial communities, with the dominant dechlorinator being Dehalococcoides in pH 7.2 and Sulf urospirillum in pH 5.5 cultures. PCE-to-trichloroethene- (TCE-) and PCE-to-cDCEdechlorinating isolates obtained from the ACS pH 5.5 enrichment shared 98.6%, and 98.5% 16S rRNA gene sequence similarities to Sulf urospirillum multivorans. Lastly, these findings imply that sustained Dehalococcoides activity cannot be expected in low pH (i.e., ≤ 5.5) groundwater, and organohalide-respiring Sulf urospirillum spp. are key contributors to in situ PCE reductive dechlorination under low pH conditions.},
doi = {10.1021/acs.est.7b01510},
journal = {Environmental Science and Technology},
number = 15,
volume = 51,
place = {United States},
year = {Fri Jun 30 00:00:00 EDT 2017},
month = {Fri Jun 30 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 36 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases
journal, April 2013
- Hug, Laura A.; Maphosa, Farai; Leys, David
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 368, Issue 1616
Microbial activity and distribution during enhanced contaminant dissolution from a NAPL source zone
journal, June 2008
- Amos, Benjamin K.; Suchomel, Eric J.; Pennell, Kurt D.
- Water Research, Vol. 42, Issue 12
Reductive dechlorination of tetrachloroethene by a stepwise catalysis of different organohalide respiring bacteria and reductive dehalogenases
journal, January 2011
- Maillard, Julien; Charnay, Marie-Paule; Regeard, Christophe
- Biodegradation, Vol. 22, Issue 5
Natural Attenuation in Streambed Sediment Receiving Chlorinated Solvents from Underlying Fracture Networks
journal, April 2017
- Şimşir, Burcu; Yan, Jun; Im, Jeongdae
- Environmental Science & Technology, Vol. 51, Issue 9
Metagenomic analysis of a stable trichloroethene-degrading microbial community
journal, March 2012
- Brisson, Vanessa L.; West, Kimberlee A.; Lee, Patrick KH
- The ISME Journal, Vol. 6, Issue 9
Isolation and characterization of Dehalospirillum multivorans gen. nov., sp. nov., a tetrachloroethene-utilizing, strictly anaerobic bacterium
journal, January 1995
- Scholz-Muramatsu, Heidrun; Neumann, Anke; Me�mer, Michael
- Archives of Microbiology, Vol. 163, Issue 1
Buffer Intensities and Equilibrium pH of Minerals and Soils: II. Theoretical and Actual pH of Minerals and Soils
journal, January 1974
- van Breemen, N.; Wielemaker, W. G.
- Soil Science Society of America Journal, Vol. 38, Issue 1
Functional Genotyping of Sulfurospirillum spp. in Mixed Cultures Allowed the Identification of a New Tetrachloroethene Reductive Dehalogenase
journal, August 2013
- Buttet, Géraldine F.; Holliger, Christof; Maillard, Julien
- Applied and Environmental Microbiology, Vol. 79, Issue 22
Characterization of Groundwater Microbial Communities, Dechlorinating Bacteria, and In Situ Biodegradation of Chloroethenes Along a Vertical Gradient
journal, March 2011
- Imfeld, Gwenaël; Pieper, Hanna; Shani, Noam
- Water, Air, & Soil Pollution, Vol. 221, Issue 1-4
Distribution of Organohalide-Respiring Bacteria between Solid and Aqueous Phases
journal, August 2014
- Cápiro, Natalie L.; Wang, Yonggang; Hatt, Janet K.
- Environmental Science & Technology, Vol. 48, Issue 18
Enhanced Reductive Dechlorination of Tetrachloroethene Dense Nonaqueous Phase Liquid with EVO and Mg(OH) 2
journal, December 2013
- Hiortdahl, Kirsten M.; Borden, Robert C.
- Environmental Science & Technology, Vol. 48, Issue 1
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
journal, June 2013
- Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.
- Applied and Environmental Microbiology, Vol. 79, Issue 17
Characterization of Two Tetrachloroethene-Reducing, Acetate-Oxidizing Anaerobic Bacteria and Their Description as Desulfuromonas michiganensis sp. nov
journal, May 2003
- Sung, Youlboong; Ritalahti, Kirsti M.; Sanford, Robert A.
- Applied and Environmental Microbiology, Vol. 69, Issue 5
Description of Sulfurospirillum halorespirans sp. nov., an anaerobic, tetrachloroethene-respiring bacterium, and transfer of Dehalospirillum multivorans to the genus Sulfurospirillum as Sulfurospirillum multivorans comb. nov.
journal, May 2003
- Luijten, M. L. G. C.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 53, Issue 3
Frequent concomitant presence of Desulfitobacterium spp. and “Dehalococcoides” spp. in chloroethene-dechlorinating microbial communities
journal, December 2010
- Rouzeau-Szynalski, Katia; Maillard, Julien; Holliger, Christof
- Applied Microbiology and Biotechnology, Vol. 90, Issue 1
Experimental Evaluation and Mathematical Modeling of Microbially Enhanced Tetrachloroethene (PCE) Dissolution
journal, February 2007
- Amos, Benjamin K.; Christ, John A.; Abriola, Linda M.
- Environmental Science & Technology, Vol. 41, Issue 3
Evaluation of Silicate Minerals for pH Control During Bioremediation: Application to Chlorinated Solvents
journal, January 2012
- Lacroix, Elsa; Brovelli, Alessandro; Holliger, Christof
- Water, Air, & Soil Pollution, Vol. 223, Issue 5
Control of groundwater pH during bioremediation: Improvement and validation of a geochemical model to assess the buffering potential of ground silicate minerals
journal, May 2014
- Lacroix, Elsa; Brovelli, Alessandro; Holliger, Christof
- Journal of Contaminant Hydrology, Vol. 160
Use of Silicate Minerals for pH Control during Reductive Dechlorination of Chloroethenes in Batch Cultures of Different Microbial Consortia
journal, April 2014
- Lacroix, Elsa; Brovelli, Alessandro; Barry, D. A.
- Applied and Environmental Microbiology, Vol. 80, Issue 13
Design tool for estimation of buffer requirement for enhanced reductive dechlorination of chlorinated solvents in groundwater
journal, November 2009
- Robinson, Clare; Barry, D. A.
- Environmental Modelling & Software, Vol. 24, Issue 11
Influence of interfaces on microbial activity.
journal, January 1990
- van Loosdrecht, M. C.; Lyklema, J.; Norde, W.
- Microbiological Reviews, Vol. 54, Issue 1
Resolution of Culture Clostridium bifermentans DPH-1 into Two Populations, a Clostridium sp. and Tetrachloroethene-Dechlorinating Desulfitobacterium hafniense Strain JH1
journal, October 2008
- Fletcher, Kelly E.; Ritalahti, Kirsti M.; Pennell, Kurt D.
- Applied and Environmental Microbiology, Vol. 74, Issue 19
Chloroethene dechlorination in acidic groundwater: Implications for combining fenton's treatment with natural attenuation
journal, January 2007
- Bradley, Paul M.; Singletary, Michael A.; Chapelle, Francis H.
- Remediation Journal, Vol. 18, Issue 1
Integrative approaches for assessing the ecological sustainability of in situ bioremediation
journal, March 2009
- Pandey, Janmejay; Chauhan, Archana; Jain, Rakesh K.
- FEMS Microbiology Reviews, Vol. 33, Issue 2
Geobacter lovleyi sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium
journal, April 2006
- Sung, Y.; Fletcher, K. E.; Ritalahti, K. M.
- Applied and Environmental Microbiology, Vol. 72, Issue 4
Biodegradation of cis -Dichloroethene as the Sole Carbon Source by a β-Proteobacterium
journal, June 2002
- Coleman, Nicholas V.; Mattes, Timothy E.; Gossett, James M.
- Applied and Environmental Microbiology, Vol. 68, Issue 6
Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi
journal, April 2012
- Loffler, F. E.; Yan, J.; Ritalahti, K. M.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 63, Issue Pt 2
Biological reduction of chlorinated solvents: Batch-scale geochemical modeling
journal, September 2010
- Kouznetsova, Irina; Mao, Xiaomin; Robinson, Clare
- Advances in Water Resources, Vol. 33, Issue 9
Optimal Treatment Zone Moves During Enhanced Reductive Dechlorination in Fractured Bedrock: Optimal Treatment Zone Moves During Enhanced Reductive Dechlorination
journal, September 2015
- Henterly, Richard W.; Harms, Willard D.
- Remediation Journal, Vol. 25, Issue 4
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012
- Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
- Nucleic Acids Research, Vol. 41, Issue D1
MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform
journal, July 2002
- Katoh, K.
- Nucleic Acids Research, Vol. 30, Issue 14
Pelosinus defluvii sp. nov., isolated from chlorinated solvent-contaminated groundwater, emended description of the genus Pelosinus and transfer of Sporotalea propionica to Pelosinus propionicus comb. nov.
journal, August 2011
- Moe, W. M.; Stebbing, R. E.; Rao, J. U.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 6
Independence of Nitrate and Nitrite Inhibition of Desulfovibrio vulgaris Hildenborough and Use of Nitrite as a Substrate for Growth
journal, January 2015
- Korte, Hannah L.; Saini, Avneesh; Trotter, Valentine V.
- Environmental Science & Technology, Vol. 49, Issue 2
Transfer of Peptostreptococcus elsdenii Gutierrez et al. to a New Genus, Megasphaera [M. elsdenii (Gutierrez et al.) comb. nov.]
journal, April 1971
- Rogosa, M.
- International Journal of Systematic Bacteriology, Vol. 21, Issue 2
Analysis of acidity production during enhanced reductive dechlorination using a simplified reactive transport model
journal, July 2012
- Brovelli, A.; Barry, D. A.; Robinson, C.
- Advances in Water Resources, Vol. 43
Acidification due to microbial dechlorination near a trichloroethene DNAPL is overcome with pH buffer or formate as electron donor: Experimental demonstration in diffusion-cells
journal, April 2013
- Philips, Jo; Maes, Nele; Springael, Dirk
- Journal of Contaminant Hydrology, Vol. 147
Isolation and characterization of Sulfurospirillum carboxydovorans sp. nov., a new microaerophilic carbon monoxide oxidizing epsilon Proteobacterium
journal, May 2005
- Jensen, Anders; Finster, Kai
- Antonie van Leeuwenhoek, Vol. 87, Issue 4
Isolation and Characterization of Desulfitobacterium sp. strain Y51 Capable of Efficient Dehalogenation of Tetrachloroethene and Polychloroethanes
journal, January 2001
- Suyama, Akiko; Iwakiri, Ryo; Kai, Keiichirou
- Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 7
Large-scale production of bacterial consortia for remediation of chlorinated solvent-contaminated groundwater
journal, June 2009
- Vainberg, Simon; Condee, Charles W.; Steffan, Robert J.
- Journal of Industrial Microbiology & Biotechnology, Vol. 36, Issue 9
Aliphatic organochlorine degradation in subsurface environments
journal, August 2014
- Koenig, Joanna; Lee, Matthew; Manefield, Mike
- Reviews in Environmental Science and Bio/Technology, Vol. 14, Issue 1
Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater
journal, November 2007
- McCarty, Perry L.; Chu, Min-Ying; Kitanidis, Peter K.
- European Journal of Soil Biology, Vol. 43, Issue 5-6
The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
journal, April 2004
- Heidelberg, John F.; Seshadri, Rekha; Haveman, Shelley A.
- Nature Biotechnology, Vol. 22, Issue 5
Activity of Desulfitobacterium sp. Strain Viet1 Demonstrates Bioavailability of 2,4-Dichlorophenol Previously Sequestered by the Aquatic Plant Lemna minor
journal, December 2005
- Tront, Jacqueline M.; Amos, Benjamin K.; Löffler, Frank E.
- Environmental Science & Technology, Vol. 40, Issue 2
Energetic aspects of malate and lactate fermentation by Acetobacterium malicum
journal, December 1991
- Strohhäcker, Joachim; Schink, Bernhard
- FEMS Microbiology Letters, Vol. 90, Issue 1
The Chimeric Genome of Sphaerochaeta : Nonspiral Spirochetes That Break with the Prevalent Dogma in Spirochete Biology
journal, July 2012
- Caro-Quintero, A.; Ritalahti, K. M.; Cusick, K. D.
- mBio, Vol. 3, Issue 3
pH control for enhanced reductive bioremediation of chlorinated solvent source zones
journal, August 2009
- Robinson, Clare; Barry, D. A.; McCarty, Perry L.
- Science of The Total Environment, Vol. 407, Issue 16
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review
journal, September 1998
- Giller, Ken E.; Witter, Ernst; Mcgrath, Steve P.
- Soil Biology and Biochemistry, Vol. 30, Issue 10-11
Sulfurospirillum cavolei sp. nov., a facultatively anaerobic sulfur-reducing bacterium isolated from an underground crude oil storage cavity
journal, April 2007
- Kodama, Yumiko; Ha, Le Thu; Watanabe, Kazuya
- International Journal of Systematic and Evolutionary Microbiology, Vol. 57, Issue 4
Hydrogen Ion Buffers for Biological Research *
journal, February 1966
- Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina
- Biochemistry, Vol. 5, Issue 2
Influence of interfaces on microbial activity
journal, March 1990
- van Loosdrecht, M. C.; Lyklema, J.; Norde, W.
- Microbiological Reviews, Vol. 54, Issue 1
Transformation yields of chlorinated ethenes by a methanotrophic mixed culture expressing particulate methane monooxygenase
journal, February 1997
- Anderson, J. E.; McCarty, P. L.
- Applied and Environmental Microbiology, Vol. 63, Issue 2
Nitrate Reduction in a Sulfate-Reducing Bacterium, Desulfovibrio desulfuricans, Isolated from Rice Paddy Soil: Sulfide Inhibition, Kinetics, and Regulation
journal, January 1994
- Dalsgaard, Tage; Bak, Friedhelm
- Applied and Environmental Microbiology, Vol. 60, Issue 1
Works referencing / citing this record:
Sanierung eines PCE-Schadens in einem makroskopisch oxischen Grundwasserleiter durch Stimulation anaerober dehalogenierender Bakterien
journal, February 2019
- Buchner, Daniel; Schweikhart, Meenakshi; Behrens, Sebastian
- Grundwasser, Vol. 24, Issue 1
Elucidating the dechlorination mechanism of hexachloroethane by Pd-doped zerovalent iron microparticles in dissolved lactic acid polymers using chromatography and indirect monitoring of iron corrosion
journal, January 2019
- Rodrigues, Romain; Betelu, Stéphanie; Colombano, Stéfan
- Environmental Science and Pollution Research, Vol. 26, Issue 7
Isolation, characterization and bioaugmentation of an acidotolerant 1,2-dichloroethane respiring Desulfitobacterium species from a low pH aquifer
journal, April 2019
- Low, Adrian; Zhao, Siyan; Rogers, Matthew J.
- FEMS Microbiology Ecology, Vol. 95, Issue 5
Sustained Dechlorination of Vinyl Chloride to Ethene in Dehalococcoides -Enriched Cultures Grown without Addition of Exogenous Vitamins and at Low pH
journal, August 2019
- Puentes Jácome, Luz A.; Wang, Po-Hsiang; Molenda, Olivia
- Environmental Science & Technology, Vol. 53, Issue 19
Targeted detection of Dehalococcoides mccartyi microbial protein biomarkers as indicators of reductive dechlorination activity in contaminated groundwater
journal, July 2019
- Solis, Manuel I. Villalobos; Abraham, Paul E.; Chourey, Karuna
- Scientific Reports, Vol. 9, Issue 1