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Title: Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions

Abstract

Bioremediation at chlorinated solvent sites often leads to groundwater acidification due to electron donor fermentation and enhanced dechlorination activity. The microbial reductive dechlorination process is robust at circumneutral pH, but activity declines at groundwater pH values below 6.0. Consistent with this observation, the activity of tetrachloroethene (PCE) dechlorinating cultures declined at pH 6.0 and was not sustained in pH 5.5 medium, with one notable exception. Sulf urospirillum multivorans dechlorinated PCE to cis-1,2-dichloroethene (cDCE) in pH 5.5 medium and maintained this activity upon repeated transfers. Microcosms established with soil and aquifer materials from five distinct locations dechlorinated PCE-to-ethene at pH 5.5 and pH 7.2. Dechlorination to ethene was maintained following repeated transfers at pH 7.2, but no ethene was produced at pH 5.5, and only the transfer cultures derived from the Axton Cross Superfund (ACS) microcosms sustained PCE dechlorination to cDCE as a final product. 16S rRNA gene amplicon sequencing of pH 7.2 and pH 5.5 ACS enrichments revealed distinct microbial communities, with the dominant dechlorinator being Dehalococcoides in pH 7.2 and Sulf urospirillum in pH 5.5 cultures. PCE-to-trichloroethene- (TCE-) and PCE-to-cDCEdechlorinating isolates obtained from the ACS pH 5.5 enrichment shared 98.6%, and 98.5% 16S rRNA gene sequence similarities to Sulfmore » urospirillum multivorans. Lastly, these findings imply that sustained Dehalococcoides activity cannot be expected in low pH (i.e., ≤ 5.5) groundwater, and organohalide-respiring Sulf urospirillum spp. are key contributors to in situ PCE reductive dechlorination under low pH conditions.« less

Authors:
 [1];  [2]; ORCiD logo [2];  [3];  [2]; ORCiD logo [4]
  1. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS)
  2. Tufts Univ., Medford, MA (United States). Dept. of Civil and Environmental Engineering
  3. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Microbiology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS)
  4. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, Dept. of Microbiology, Dept. of Biosystems Engineering and Soil Science; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, and joint Inst. for Biological Sciences (JIBS)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; USDoD
OSTI Identifier:
1399396
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 51; Journal Issue: 15; Journal ID: ISSN 0013-936X
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., and Löffler, Frank E. Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions. United States: N. p., 2017. Web. https://doi.org/10.1021/acs.est.7b01510.
Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., & Löffler, Frank E. Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions. United States. https://doi.org/10.1021/acs.est.7b01510
Yang, Yi, Cápiro, Natalie L., Marcet, Tyler F., Yan, Jun, Pennell, Kurt D., and Löffler, Frank E. Fri . "Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions". United States. https://doi.org/10.1021/acs.est.7b01510. https://www.osti.gov/servlets/purl/1399396.
@article{osti_1399396,
title = {Organohalide Respiration with Chlorinated Ethenes under Low pH Conditions},
author = {Yang, Yi and Cápiro, Natalie L. and Marcet, Tyler F. and Yan, Jun and Pennell, Kurt D. and Löffler, Frank E.},
abstractNote = {Bioremediation at chlorinated solvent sites often leads to groundwater acidification due to electron donor fermentation and enhanced dechlorination activity. The microbial reductive dechlorination process is robust at circumneutral pH, but activity declines at groundwater pH values below 6.0. Consistent with this observation, the activity of tetrachloroethene (PCE) dechlorinating cultures declined at pH 6.0 and was not sustained in pH 5.5 medium, with one notable exception. Sulf urospirillum multivorans dechlorinated PCE to cis-1,2-dichloroethene (cDCE) in pH 5.5 medium and maintained this activity upon repeated transfers. Microcosms established with soil and aquifer materials from five distinct locations dechlorinated PCE-to-ethene at pH 5.5 and pH 7.2. Dechlorination to ethene was maintained following repeated transfers at pH 7.2, but no ethene was produced at pH 5.5, and only the transfer cultures derived from the Axton Cross Superfund (ACS) microcosms sustained PCE dechlorination to cDCE as a final product. 16S rRNA gene amplicon sequencing of pH 7.2 and pH 5.5 ACS enrichments revealed distinct microbial communities, with the dominant dechlorinator being Dehalococcoides in pH 7.2 and Sulf urospirillum in pH 5.5 cultures. PCE-to-trichloroethene- (TCE-) and PCE-to-cDCEdechlorinating isolates obtained from the ACS pH 5.5 enrichment shared 98.6%, and 98.5% 16S rRNA gene sequence similarities to Sulf urospirillum multivorans. Lastly, these findings imply that sustained Dehalococcoides activity cannot be expected in low pH (i.e., ≤ 5.5) groundwater, and organohalide-respiring Sulf urospirillum spp. are key contributors to in situ PCE reductive dechlorination under low pH conditions.},
doi = {10.1021/acs.est.7b01510},
journal = {Environmental Science and Technology},
number = 15,
volume = 51,
place = {United States},
year = {2017},
month = {6}
}

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