‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family
Abstract
Taxonomic assignments of anaerobic dichloromethane (DCM)-degrading bacteria remain poorly constrained but are important for understanding the microbial diversity of organisms contributing to DCM turnover in environmental systems. Here, we describe the taxonomic classification of a novel DCM degrader in consortium RM obtained from pristine Rio Mameyes sediment. Phylogenetic analysis of full-length 16S rRNA gene sequences demonstrated that the DCM degrader was most closely related to members of the genera Dehalobacter and Syntrophobotulus, but sequence similarities did not exceed 94% and 93%, respectively. Genome-aggregate average amino acid identities against Peptococcaceae members did not exceed 66%, suggesting that the DCM degrader does not affiliate with any described genus. Phylogenetic analysis of conserved single-copy functional genes supported that the DCM degrader represents a novel clade. Growth strictly depended on the presence of DCM, which was consumed at a rate of 160 ± 3 μmol L-1 d-1. The DCM degrader attained 5.25 × 107 ± 1.0 × 107 cells per μmol DCM consumed. Fluorescence in situ hybridization revealed rod-shaped cells 4 ± 0.8 μm long and 0.4 ± 0.1 μm wide. Furthermore, based on the unique phylogenetic, genomic, and physiological characteristics, we propose that the DCM degrader represents a new genus and species, ‘Candidatusmore »
- Authors:
-
[1];
[6]
- Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering, School of Biology
- Corporate Remediation Group, Newark, DE (United States)
- Univ. of Tennessee and Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. for Biological Sciences (JIBS); Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1399390
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Systematic and Applied Microbiology
- Additional Journal Information:
- Journal Volume: 40; Journal Issue: 3; Journal ID: ISSN 0723-2020
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 96 KNOWLEDGE MANAGEMENT AND PRESERVATION; anaerobic dichloromethane degradation; candidatus dichloromethanomonas elyunquensis; peptococcaceae; metagenome binning
Citation Formats
Kleindienst, Sara, Higgins, Steven A., Tsementzi, Despina, Chen, Gao, Konstantinidis, Konstantinos T., Mack, E. Erin, and Löffler, Frank E. ‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family. United States: N. p., 2016.
Web. doi:10.1016/j.syapm.2016.12.001.
Kleindienst, Sara, Higgins, Steven A., Tsementzi, Despina, Chen, Gao, Konstantinidis, Konstantinos T., Mack, E. Erin, & Löffler, Frank E. ‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family. United States. https://doi.org/10.1016/j.syapm.2016.12.001
Kleindienst, Sara, Higgins, Steven A., Tsementzi, Despina, Chen, Gao, Konstantinidis, Konstantinos T., Mack, E. Erin, and Löffler, Frank E. Wed .
"‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family". United States. https://doi.org/10.1016/j.syapm.2016.12.001. https://www.osti.gov/servlets/purl/1399390.
@article{osti_1399390,
title = {‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family},
author = {Kleindienst, Sara and Higgins, Steven A. and Tsementzi, Despina and Chen, Gao and Konstantinidis, Konstantinos T. and Mack, E. Erin and Löffler, Frank E.},
abstractNote = {Taxonomic assignments of anaerobic dichloromethane (DCM)-degrading bacteria remain poorly constrained but are important for understanding the microbial diversity of organisms contributing to DCM turnover in environmental systems. Here, we describe the taxonomic classification of a novel DCM degrader in consortium RM obtained from pristine Rio Mameyes sediment. Phylogenetic analysis of full-length 16S rRNA gene sequences demonstrated that the DCM degrader was most closely related to members of the genera Dehalobacter and Syntrophobotulus, but sequence similarities did not exceed 94% and 93%, respectively. Genome-aggregate average amino acid identities against Peptococcaceae members did not exceed 66%, suggesting that the DCM degrader does not affiliate with any described genus. Phylogenetic analysis of conserved single-copy functional genes supported that the DCM degrader represents a novel clade. Growth strictly depended on the presence of DCM, which was consumed at a rate of 160 ± 3 μmol L-1 d-1. The DCM degrader attained 5.25 × 107 ± 1.0 × 107 cells per μmol DCM consumed. Fluorescence in situ hybridization revealed rod-shaped cells 4 ± 0.8 μm long and 0.4 ± 0.1 μm wide. Furthermore, based on the unique phylogenetic, genomic, and physiological characteristics, we propose that the DCM degrader represents a new genus and species, ‘Candidatus Dichloromethanomonas elyunquensis’.},
doi = {10.1016/j.syapm.2016.12.001},
journal = {Systematic and Applied Microbiology},
number = 3,
volume = 40,
place = {United States},
year = {Wed Dec 21 00:00:00 EST 2016},
month = {Wed Dec 21 00:00:00 EST 2016}
}
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Works referencing / citing this record:
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