Switchgrass rhizospheres stimulate microbial biomass but deplete microbial necromass in agricultural soils of the upper Midwest, USA
Abstract
Rhizosphere microbial communities play an essential role in determining plant productivity, particularly in agriculturally marginal environments. Perennial plants like switchgrass (Panicum virgatum) are thought to particularly influence microbial community composition and function within their rhizosphere. We compared microbial communities in switchgrass rhizospheres and their associated bulk soils in two regions of the U.S. upper Midwest (Michigan and Wisconsin) with contrasting soil types, and at two site types with differing switchgrass establishment ages and management intensities. We characterized microbial communities with a range of culture-independent methods, including amplicon sequencing of 16S/18S rRNA and nifH genes, and membrane lipid profiling. In addition, we quantified abundances of soil amino sugars, a time-integrative indicator of microbial necromass. We found that amino sugar contents and microbial lipid profiles differed between rhizosphere and bulk soils, while DNA-based assays did not provide this discriminatory power. Differences between rhizosphere and bulk soils were not significantly affected by region or site type. Rhizosphere soils had higher microbial lipid abundances, particularly those associated with arbuscular mycorrhizal fungi and Gram-negative bacteria, while amino sugar abundances decreased in the rhizosphere. In conclusion, our findings suggest switchgrass rhizospheres systematically stimulate microbial growth and microbial residue turnover.
- Authors:
-
- University of Wisconsin, Madison, WI (United States); Chinese Academy of Sciences (CAS), Shenyang (China)
- University of Wisconsin, Madison, WI (United States); Michigan State University, East Lansing, MI (United States)
- University of Wisconsin, Madison, WI (United States)
- Michigan State University, East Lansing, MI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1418539
- Alternate Identifier(s):
- OSTI ID: 1399029
- Grant/Contract Number:
- FC02-07ER64494
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Soil Biology and Biochemistry
- Additional Journal Information:
- Journal Volume: 94; Journal Issue: C; Journal ID: ISSN 0038-0717
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; pyrosequencing; nifH; lipid; amino sugar; plant–microbe interaction; rhizosphere; switchgrass
Citation Formats
Liang, Chao, Jesus, Ederson da C., Duncan, David S., Quensen, John F., Jackson, Randall D., Balser, Teri C., and Tiedje, James M. Switchgrass rhizospheres stimulate microbial biomass but deplete microbial necromass in agricultural soils of the upper Midwest, USA. United States: N. p., 2015.
Web. doi:10.1016/j.soilbio.2015.11.020.
Liang, Chao, Jesus, Ederson da C., Duncan, David S., Quensen, John F., Jackson, Randall D., Balser, Teri C., & Tiedje, James M. Switchgrass rhizospheres stimulate microbial biomass but deplete microbial necromass in agricultural soils of the upper Midwest, USA. United States. https://doi.org/10.1016/j.soilbio.2015.11.020
Liang, Chao, Jesus, Ederson da C., Duncan, David S., Quensen, John F., Jackson, Randall D., Balser, Teri C., and Tiedje, James M. Sat .
"Switchgrass rhizospheres stimulate microbial biomass but deplete microbial necromass in agricultural soils of the upper Midwest, USA". United States. https://doi.org/10.1016/j.soilbio.2015.11.020. https://www.osti.gov/servlets/purl/1418539.
@article{osti_1418539,
title = {Switchgrass rhizospheres stimulate microbial biomass but deplete microbial necromass in agricultural soils of the upper Midwest, USA},
author = {Liang, Chao and Jesus, Ederson da C. and Duncan, David S. and Quensen, John F. and Jackson, Randall D. and Balser, Teri C. and Tiedje, James M.},
abstractNote = {Rhizosphere microbial communities play an essential role in determining plant productivity, particularly in agriculturally marginal environments. Perennial plants like switchgrass (Panicum virgatum) are thought to particularly influence microbial community composition and function within their rhizosphere. We compared microbial communities in switchgrass rhizospheres and their associated bulk soils in two regions of the U.S. upper Midwest (Michigan and Wisconsin) with contrasting soil types, and at two site types with differing switchgrass establishment ages and management intensities. We characterized microbial communities with a range of culture-independent methods, including amplicon sequencing of 16S/18S rRNA and nifH genes, and membrane lipid profiling. In addition, we quantified abundances of soil amino sugars, a time-integrative indicator of microbial necromass. We found that amino sugar contents and microbial lipid profiles differed between rhizosphere and bulk soils, while DNA-based assays did not provide this discriminatory power. Differences between rhizosphere and bulk soils were not significantly affected by region or site type. Rhizosphere soils had higher microbial lipid abundances, particularly those associated with arbuscular mycorrhizal fungi and Gram-negative bacteria, while amino sugar abundances decreased in the rhizosphere. In conclusion, our findings suggest switchgrass rhizospheres systematically stimulate microbial growth and microbial residue turnover.},
doi = {10.1016/j.soilbio.2015.11.020},
journal = {Soil Biology and Biochemistry},
number = C,
volume = 94,
place = {United States},
year = {Sat Dec 19 00:00:00 EST 2015},
month = {Sat Dec 19 00:00:00 EST 2015}
}
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