Exception to the Rule: Genomic Characterization of Naturally Occurring Unusual Vibrio cholerae Strains with a Single Chromosome

Xie, Gary; Johnson, Shannon Lyn; Davenport, Karen Walston; Rajavel, Mathumathi; Waldminghaus, Torsten; Detter, John Chris; Chain, Patrick Sam Guy; Sozhamannan, Shanmuga

doi:10.1155/2017/8724304

Title: Exception to the Rule: Genomic Characterization of Naturally Occurring Unusual Vibrio cholerae Strains with a Single Chromosome

Journal Article · 29 August 2017 · International Journal of Genomics

DOI: https://doi.org/10.1155/2017/8724304 · OSTI ID:1398918

^[1];

^[1]; Davenport, Karen Walston ^[1]; Rajavel, Mathumathi ^[2]; Waldminghaus, Torsten ^[3]; Detter, John Chris ^[1]; Chain, Patrick Sam Guy ^[1];

^[4]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Morgan State Univ., Baltimore, MD (United States)
Philipps-Univ. Marburg, Marburg (Germany)
Tauri Group, LLC, Alexandria, VA (United States); Defense Biological Product Assurance Office, Frederick, MD (United States)

Here, the genetic make-up of most bacteria is encoded in a single chromosome while about 10% have more than one chromosome. Among these, Vibrio cholerae, with two chromosomes, has served as a model system to study various aspects of chromosome maintenance, mainly replication, and faithful partitioning of multipartite genomes. Here, we describe the genomic characterization of strains that are an exception to the two chromosome rules: naturally occurring single-chromosome V. cholerae. Whole genome sequence analyses of NSCV1 and NSCV2 (natural single-chromosome vibrio) revealed that the Chr1 and Chr2 fusion junctions contain prophages, IS elements, and direct repeats, in addition to large-scale chromosomal rearrangements such as inversions, insertions, and long tandem repeats elsewhere in the chromosome compared to prototypical two chromosome V. cholerae genomes. Many of the known cholera virulence factors are absent. The two origins of replication and associated genes are generally intact with synonymous mutations in some genes, as arerecAand mismatch repair (MMR) genes dam, mutH, and mutL; MutS function is probably impaired in NSCV2. These strains are ideal tools for studying mechanistic aspects of maintenance of chromosomes with multiple origins and other rearrangements and the biological, functional, and evolutionary significance of multipartite genome architecture in general.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: Defense Threat Reduction Agency (DTRA); USDOE

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1398918

Report Number(s):: LA-UR-17-21600

Journal Information:: International Journal of Genomics, Vol. 2017; ISSN 2314-436X

Publisher:: HindawiCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 15 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Biological Science
V. cholerae
non-O1/non-O139 serogroup
whole genome sequencing
single chromosome
DNA adenine methyl transferase
dam
origin of replication
mismatch repair

Title: Exception to the Rule: Genomic Characterization of Naturally Occurring Unusual Vibrio cholerae Strains with a Single Chromosome

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