Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype
Abstract
Clostridium beijerinckii is a predominant solventogenic bacterium that is used for the ABE fermentation. Various C. beijerinckii mutants are constructed for desirable phenotypes. The C. beijerinckii mutant BA105 harboring a glucose derepression phenotype was previously isolated and demonstrated the enhanced amylolytic activity in the presence of glucose. Despite its potential use, BA105 is not further characterized and utilized. Therefore, the authors investigate fermentation phenotypes of BA105 in this study. Under the typical batch fermentation conditions, BA105 consistently exhibits acid crash phenotype resulting in limited glucose uptake and cell growth. However, when the culture pH is maintained above 5.5, BA105 exhibits the increased glucose uptake and butanol production than did the wild‐type. To further analyze BA105, the authors perform genome sequencing and RNA sequencing. Genome analysis identifies two SNPs unique to BA105, in the upstream region of AbrB regulator (Cbei_4885) and the ROK family glucokinase (Cbei_4895) which are involved in catabolite repression and regulation of sugar metabolism. Transcriptional analysis of BA105 reveals significant differential expression of the genes associated with the PTS sugar transport system and acid production. This study improves understanding of the acid crash phenomenon and provides the genetic basis underlying the catabolite derepression phenotype of C. beijericnkii .
- Authors:
-
[1];
- Department of Food Science and Human Nutrition University of Illinois at Urbana‐Champaign Urbana IL 61801 USA, Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana‐Champaign Urbana IL 61801 USA
- Department of Food Science and Human Nutrition University of Illinois at Urbana‐Champaign Urbana IL 61801 USA
- Institute for Systems Biology Seattle WA USA
- Biosystems Engineering Department Auburn University Auburn AL 36849 USA
- Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana‐Champaign Urbana IL 61801 USA, Department of Bioengineering and Department of Physics University of Illinois at Urbana‐Champaign Urbana IL 61801 USA
- Department of Food Science and Human Nutrition University of Illinois at Urbana‐Champaign Urbana IL 61801 USA, The Integrated Bioprocessing Research Laboratory (IBRL) University of Illinois at Urbana‐Champaign Urbana IL 61801 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1398726
- Grant/Contract Number:
- #2011‐01219
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Biotechnology Journal
- Additional Journal Information:
- Journal Name: Biotechnology Journal Journal Volume: 12 Journal Issue: 11; Journal ID: ISSN 1860-6768
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Germany
- Language:
- English
Citation Formats
Seo, Seung‐Oh, Janssen, Holger, Magis, Andrew, Wang, Yi, Lu, Ting, Price, Nathan D., Jin, Yong‐Su, and Blaschek, Hans P. Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype. Germany: N. p., 2017.
Web. doi:10.1002/biot.201700182.
Seo, Seung‐Oh, Janssen, Holger, Magis, Andrew, Wang, Yi, Lu, Ting, Price, Nathan D., Jin, Yong‐Su, & Blaschek, Hans P. Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype. Germany. https://doi.org/10.1002/biot.201700182
Seo, Seung‐Oh, Janssen, Holger, Magis, Andrew, Wang, Yi, Lu, Ting, Price, Nathan D., Jin, Yong‐Su, and Blaschek, Hans P. Mon .
"Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype". Germany. https://doi.org/10.1002/biot.201700182.
@article{osti_1398726,
title = {Genomic, Transcriptional, and Phenotypic Analysis of the Glucose Derepressed Clostridium beijerinckii Mutant Exhibiting Acid Crash Phenotype},
author = {Seo, Seung‐Oh and Janssen, Holger and Magis, Andrew and Wang, Yi and Lu, Ting and Price, Nathan D. and Jin, Yong‐Su and Blaschek, Hans P.},
abstractNote = {Clostridium beijerinckii is a predominant solventogenic bacterium that is used for the ABE fermentation. Various C. beijerinckii mutants are constructed for desirable phenotypes. The C. beijerinckii mutant BA105 harboring a glucose derepression phenotype was previously isolated and demonstrated the enhanced amylolytic activity in the presence of glucose. Despite its potential use, BA105 is not further characterized and utilized. Therefore, the authors investigate fermentation phenotypes of BA105 in this study. Under the typical batch fermentation conditions, BA105 consistently exhibits acid crash phenotype resulting in limited glucose uptake and cell growth. However, when the culture pH is maintained above 5.5, BA105 exhibits the increased glucose uptake and butanol production than did the wild‐type. To further analyze BA105, the authors perform genome sequencing and RNA sequencing. Genome analysis identifies two SNPs unique to BA105, in the upstream region of AbrB regulator (Cbei_4885) and the ROK family glucokinase (Cbei_4895) which are involved in catabolite repression and regulation of sugar metabolism. Transcriptional analysis of BA105 reveals significant differential expression of the genes associated with the PTS sugar transport system and acid production. This study improves understanding of the acid crash phenomenon and provides the genetic basis underlying the catabolite derepression phenotype of C. beijericnkii .},
doi = {10.1002/biot.201700182},
journal = {Biotechnology Journal},
number = 11,
volume = 12,
place = {Germany},
year = {Mon Oct 09 00:00:00 EDT 2017},
month = {Mon Oct 09 00:00:00 EDT 2017}
}
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