Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers
Abstract
To better understand animal cell plasma membranes, we studied simplified models, namely four-component lipid bilayer mixtures. Here we describe the domain size transition in the region of coexisting liquid-disordered (Ld) + liquid-ordered (Lo) phases. This transition occurs abruptly in composition space with domains increasing in size by two orders of magnitude, from tens of nanometers to microns. We measured the line tension between coexisting Ld and Lo domains close to the domain size transition for a variety of lipid mixtures, finding that in every case the transition occurs at a line tension of ~0.3 pN. A computational model incorporating line tension and dipole repulsion indicated that even small changes in line tension can result in domains growing in size by several orders of magnitude, consistent with experimental observations. Lastly, we find that other properties of the coexisting Ld and Lo phases do not change significantly in the vicinity of the abrupt domain size transition.
- Authors:
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). High Flux Isotope Reactor (HFIR); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1432720
- Alternate Identifier(s):
- OSTI ID: 1351775; OSTI ID: 1398313
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Biophysical Journal
- Additional Journal Information:
- Journal Name: Biophysical Journal Journal Volume: 112 Journal Issue: 7; Journal ID: ISSN 0006-3495
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Usery, Rebecca D., Enoki, Thais A., Wickramasinghe, Sanjula P., Weiner, Michael D., Tsai, Wen-Chyan, Kim, Mary B., Wang, Shu, Torng, Thomas L., Ackerman, David G., Heberle, Frederick A., Katsaras, John, and Feigenson, Gerald W. Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers. United States: N. p., 2017.
Web. doi:10.1016/j.bpj.2017.02.033.
Usery, Rebecca D., Enoki, Thais A., Wickramasinghe, Sanjula P., Weiner, Michael D., Tsai, Wen-Chyan, Kim, Mary B., Wang, Shu, Torng, Thomas L., Ackerman, David G., Heberle, Frederick A., Katsaras, John, & Feigenson, Gerald W. Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers. United States. https://doi.org/10.1016/j.bpj.2017.02.033
Usery, Rebecca D., Enoki, Thais A., Wickramasinghe, Sanjula P., Weiner, Michael D., Tsai, Wen-Chyan, Kim, Mary B., Wang, Shu, Torng, Thomas L., Ackerman, David G., Heberle, Frederick A., Katsaras, John, and Feigenson, Gerald W. Sat .
"Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers". United States. https://doi.org/10.1016/j.bpj.2017.02.033.
@article{osti_1432720,
title = {Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers},
author = {Usery, Rebecca D. and Enoki, Thais A. and Wickramasinghe, Sanjula P. and Weiner, Michael D. and Tsai, Wen-Chyan and Kim, Mary B. and Wang, Shu and Torng, Thomas L. and Ackerman, David G. and Heberle, Frederick A. and Katsaras, John and Feigenson, Gerald W.},
abstractNote = {To better understand animal cell plasma membranes, we studied simplified models, namely four-component lipid bilayer mixtures. Here we describe the domain size transition in the region of coexisting liquid-disordered (Ld) + liquid-ordered (Lo) phases. This transition occurs abruptly in composition space with domains increasing in size by two orders of magnitude, from tens of nanometers to microns. We measured the line tension between coexisting Ld and Lo domains close to the domain size transition for a variety of lipid mixtures, finding that in every case the transition occurs at a line tension of ~0.3 pN. A computational model incorporating line tension and dipole repulsion indicated that even small changes in line tension can result in domains growing in size by several orders of magnitude, consistent with experimental observations. Lastly, we find that other properties of the coexisting Ld and Lo phases do not change significantly in the vicinity of the abrupt domain size transition.},
doi = {10.1016/j.bpj.2017.02.033},
journal = {Biophysical Journal},
number = 7,
volume = 112,
place = {United States},
year = {Sat Apr 01 00:00:00 EDT 2017},
month = {Sat Apr 01 00:00:00 EDT 2017}
}
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https://doi.org/10.1016/j.bpj.2017.02.033
https://doi.org/10.1016/j.bpj.2017.02.033
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