Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli

Choi, Sylvia K.; Lin, Myat T.; Ouyang, Hanlin; Gennis, Robert B.

doi:10.1016/j.bbabio.2017.02.008

Title: Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli

Authors:: Choi, Sylvia K.; Lin, Myat T.; Ouyang, Hanlin; Gennis, Robert B.

Publication Date:: Mon May 01 00:00:00 EDT 2017

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1435465

Alternate Identifier(s):: OSTI ID: 1397641

Grant/Contract Number:: FG02-87ER13716

Resource Type:: Published Article

Journal Name:: Biochimica et Biophysica Acta - Bioenergetics

Additional Journal Information:: Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Volume: 1858 Journal Issue: 5; Journal ID: ISSN 0005-2728

Publisher:: Elsevier

Country of Publication:: Netherlands

Language:: English

Citation Formats


                    Choi, Sylvia K., Lin, Myat T., Ouyang, Hanlin, and Gennis, Robert B. Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli.  Netherlands: N. p., 2017. 
Web.  doi:10.1016/j.bbabio.2017.02.008.

Copy to clipboard


                    Choi, Sylvia K., Lin, Myat T., Ouyang, Hanlin, & Gennis, Robert B. Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli.  Netherlands.  https://doi.org/10.1016/j.bbabio.2017.02.008

Copy to clipboard


                    Choi, Sylvia K., Lin, Myat T., Ouyang, Hanlin, and Gennis, Robert B. Mon .  
"Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli".  Netherlands.  https://doi.org/10.1016/j.bbabio.2017.02.008.

Copy to clipboard


                    
@article{osti_1435465,

  title        = {Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli},

  author       = {Choi, Sylvia K. and Lin, Myat T. and Ouyang, Hanlin and Gennis, Robert B.},

  abstractNote = {},

  doi          = {10.1016/j.bbabio.2017.02.008},

  journal      = {Biochimica et Biophysica Acta - Bioenergetics},

  number       = 5,

  volume       = 1858,

  place        = {Netherlands},

  year         = {Mon May 01 00:00:00 EDT 2017},

  month        = {Mon May 01 00:00:00 EDT 2017}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Publisher's Version of Record
https://doi.org/10.1016/j.bbabio.2017.02.008

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 8 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase
journal, October 2006

Qin, L.; Hiser, C.; Mulichak, A.
Proceedings of the National Academy of Sciences, Vol. 103, Issue 44
DOI: 10.1073/pnas.0606149103

Characterization of the Ubiquinol Oxidation Sites in Cytochromes bo and bd from Escherichia coli using Aurachin C Analogues
journal, January 1999

Miyoshi, H.; Takegami, K.; Sakamoto, K.
Journal of Biochemistry, Vol. 125, Issue 1
DOI: 10.1093/oxfordjournals.jbchem.a022250

The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli
journal, December 2010

Yap, Lai Lai; Lin, Myat T.; Ouyang, Hanlin
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1797, Issue 12
DOI: 10.1016/j.bbabio.2010.04.011

On the function of the various quinone species in Escherichia coli : The role of DMK in the electron transfer chains of
journal, May 2012

Sharma, Poonam; Teixeira de Mattos, Maarten J.; Hellingwerf, Klaas J.
FEBS Journal, Vol. 279, Issue 18
DOI: 10.1111/j.1742-4658.2012.08608.x

Studies on a Stabilisation of Ubisemiquinone by Escherichia coli Quinol Oxidase, Cytochrome bo
journal, February 1995

Ingledew, W. John; Ohnishi, Tomoko; Salerno, John C.
European Journal of Biochemistry, Vol. 227, Issue 3
DOI: 10.1111/j.1432-1033.1995.tb20217.x

Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry To Map the Quinol Binding Site of Cytochrome bo ₃ from Escherichia coli ^†
journal, July 1998

Tsatsos, Panagiota H.; Reynolds, Kate; Nickels, Elizabeth Furlong
Biochemistry, Vol. 37, Issue 28
DOI: 10.1021/bi9809270

Identification of the Residues Involved in Stabilization of the Semiquinone Radical in the High-Affinity Ubiquinone Binding Site in Cytochrome bo ₃ from Escherichia coli by Site-Directed Mutagenesis and EPR Spectroscopy ^†
journal, August 2002

Hellwig, Petra; Yano, Takahiro; Ohnishi, Tomoko
Biochemistry, Vol. 41, Issue 34
DOI: 10.1021/bi012146w

Vibrational Modes of Ubiquinone in Cytochrome bo ₃ from Escherichia coli Identified by Fourier Transform Infrared Difference Spectroscopy and Specific ¹³ C Labeling ^†
journal, November 1999

Hellwig, Petra; Mogi, Tatsushi; Tomson, Farol L.
Biochemistry, Vol. 38, Issue 44
DOI: 10.1021/bi991267h

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q _H Site of Wild-Type and D75H Mutant Cytochrome bo ₃ from Escherichia coli
journal, April 2012

Lin, Myat T.; Baldansuren, Amgalanbaatar; Hart, Richard
Biochemistry, Vol. 51, Issue 18
DOI: 10.1021/bi300151q

Asymmetric Binding of the High-Affinity Q _H ^• ^- Ubisemiquinone in Quinol Oxidase ( bo ₃ ) from Escherichia coli Studied by Multifrequency Electron Paramagnetic Resonance Spectroscopy ^†
journal, May 2003

Grimaldi, S.; Ostermann, T.; Weiden, N.
Biochemistry, Vol. 42, Issue 19
DOI: 10.1021/bi034010z

Reconstitution of the Ubiquinone-dependent pyruvate oxidase system of Escherichia coli with the cytochrome o terminal oxidase complex.
journal, September 1985

Carter, K.; Gennis, R. B.
Journal of Biological Chemistry, Vol. 260, Issue 20
DOI: 10.1016/S0021-9258(17)39135-4

Cytochrome o ( bo ) is a proton pump in Paracoccus denitrificans and Escherichia coli
journal, June 1989

Puustinen, Anne; Finel, Moshe; Virkki, Marika
FEBS Letters, Vol. 249, Issue 2
DOI: 10.1016/0014-5793(89)80616-7

A study of cytochrome bo3 in a tethered bilayer lipid membrane
journal, December 2010

Weiss, Sophie A.; Bushby, Richard J.; Evans, Stephen D.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1797, Issue 12
DOI: 10.1016/j.bbabio.2010.01.012

Tryptophan-136 in Subunit II of Cytochrome bo ₃ from Escherichia c oli May Participate in the Binding of Ubiquinol ^†
journal, August 1998

Ma, Jixiang; Puustinen, Anne; Wikström, Mårten
Biochemistry, Vol. 37, Issue 34
DOI: 10.1021/bi9809977

Direct Evidence for the Protonation of Aspartate-75, Proposed To Be at a Quinol Binding Site, upon Reduction of Cytochrome bo ₃ from Escherichia coli ^†
journal, January 2001

Hellwig, Petra; Barquera, Blanca; Gennis, Robert B.
Biochemistry, Vol. 40, Issue 4
DOI: 10.1021/bi002154x

Protein−Cofactor Interactions and EPR Parameters for the Q _H Quinone Binding Site of Quinol Oxidase. A Density Functional Study
journal, May 2006

Kacprzak, Sylwia; Kaupp, Martin; MacMillan, Fraser
Journal of the American Chemical Society, Vol. 128, Issue 17
DOI: 10.1021/ja053988b

Mimicking respiratory phosphorylation using purified enzymes
journal, April 2016

von Ballmoos, Christoph; Biner, Olivier; Nilsson, Tobias
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 4
DOI: 10.1016/j.bbabio.2015.12.007

The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases.
journal, July 1990

Chepuri, V.; Lemieux, L.; Au, D. C.
Journal of Biological Chemistry, Vol. 265, Issue 19
DOI: 10.1016/S0021-9258(19)38574-6

The superfamily of heme–copper oxygen reductases: Types and evolutionary considerations
journal, April 2012

Sousa, Filipa L.; Alves, Renato J.; Ribeiro, Miguel A.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 4
DOI: 10.1016/j.bbabio.2011.09.020

A Bioinformatics Classifier and Database for Heme-Copper Oxygen Reductases
journal, April 2011

Sousa, Filipa L.; Alves, Renato J.; Pereira-Leal, José B.
PLoS ONE, Vol. 6, Issue 4
DOI: 10.1371/journal.pone.0019117

Monitoring the Transmembrane Proton Gradient Generated by Cytochrome bo ₃ in Tethered Bilayer Lipid Membranes Using SEIRA Spectroscopy
journal, February 2016

Wiebalck, Swantje; Kozuch, Jacek; Forbrig, Enrico
The Journal of Physical Chemistry B, Vol. 120, Issue 9
DOI: 10.1021/acs.jpcb.6b01435

Identification of the Nitrogen Donor Hydrogen Bonded with the Semiquinone at the Q _H Site of the Cytochrome bo ₃ from Escherichia coli
journal, November 2008

Lin, Myat T.; Samoilova, Rimma I.; Gennis, Robert B.
Journal of the American Chemical Society, Vol. 130, Issue 47
DOI: 10.1021/ja805906a

An Elementary Reaction Step of the Proton Pump Is Revealed by Mutation of Tryptophan-164 to Phenylalanine in Cytochrome c Oxidase from Paracoccus denitrificans ^†
journal, December 2005

Ribacka, Camilla; Verkhovsky, Michael I.; Belevich, Ilya
Biochemistry, Vol. 44, Issue 50
DOI: 10.1021/bi0511336

ENDOR spectroscopic studies of stable semiquinone radicals bound to the Escherichia coli cytochrome bo3 quinol oxidase: ENDOR of cytochrome bo3 semiquinones
journal, September 2000

Hastings, Stuart F.; Heathcote, Peter; Ingledew, W. John
European Journal of Biochemistry, Vol. 267, Issue 17
DOI: 10.1046/j.1432-1327.2000.01643.x

Isolation and Characterizations of Quinone Analogue-Resistant Mutants of b o -Type Ubiquinol Oxidase from Escherichia c oli ^†
journal, September 1998

Sato-Watanabe, Mariko; Mogi, Tatsushi; Sakamoto, Kimitoshi
Biochemistry, Vol. 37, Issue 37
DOI: 10.1021/bi981184l

Q-Band ENDOR (Electron Nuclear Double Resonance) of the High-Affinity Ubisemiquinone Center in Cytochrome bo ₃ from Escherichia coli ^†
journal, March 2000

Veselov, Andrei V.; Osborne, Jeffrey P.; Gennis, Robert B.
Biochemistry, Vol. 39, Issue 11
DOI: 10.1021/bi9926835

Prediction of high- and low-affinity quinol-analogue-binding sites in the aa3 and bo3 terminal oxidases from Bacillus subtilis and Escherichia coli1
journal, June 2014

Bossis, Fabrizio; De Grassi, Anna; Palese, Luigi Leonardo
Biochemical Journal, Vol. 461, Issue 2
DOI: 10.1042/BJ20140082

Subunit II of the Cytochrome bo ₃ Ubiquinol Oxidase from Escherichia coli Is a Lipoprotein
journal, September 1997

Ma, Jixiang; Katsonouri, Andromachi; Gennis, Robert B.
Biochemistry, Vol. 36, Issue 38
DOI: 10.1021/bi9709710

Stabilization of a semiquinone radical at the high-affinity quinone-binding site (Q _H ) of the Escherichia coli bo -type ubiquinol oxidase
journal, October 1995

Sato-Watanabe, Mariko; Itoh, Sigeru; Mogi, Tatsushi
FEBS Letters, Vol. 374, Issue 2
DOI: 10.1016/0014-5793(95)01125-X

Role of a bound ubiquinone on reactions of the Escherichia coli cytochrome bo with ubiquinol and dioxygen
journal, August 1999

Mogi, Tatsushi; Sato-Watanabe, Mariko; Miyoshi, Hideto
FEBS Letters, Vol. 457, Issue 1
DOI: 10.1016/S0014-5793(99)01007-8

Characterization of Mutants That Change the Hydrogen Bonding of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli*
journal, March 2007

Yap, Lai Lai; Samoilova, Rimma I.; Gennis, Robert B.
Journal of Biological Chemistry, Vol. 282, Issue 12
DOI: 10.1074/jbc.M611595200

Uncompetitive Substrate Inhibition and Noncompetitive Inhibition by 5- n -Undecyl-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and 2- n -Nonyl-4-hydroxyquinoline- N -oxide (NQNO) is Observed for the Cytochrome bo ₃ Complex: Implications for a Q(H ₂ )-Loop Proton Translocation Mechanism ^†
journal, January 1997

Musser, Siegfried M.; Stowell, Michael H. B.; Lee, Hung Kay
Biochemistry, Vol. 36, Issue 4
DOI: 10.1021/bi961723r

Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols.
journal, November 1994

Sato-Watanabe, M.; Mogi, T.; Miyoshi, H.
Journal of Biological Chemistry, Vol. 269, Issue 46
DOI: 10.1016/S0021-9258(19)61992-7

Q_H^•- Ubisemiquinone Radical in the bo₃-Type Ubiquinol Oxidase Studied by Pulsed Electron Paramagnetic Resonance and Hyperfine Sublevel Correlation Spectroscopy
journal, January 2001

Grimaldi, Stéphane; MacMillan, Fraser; Ostermann, Thomas
Biochemistry, Vol. 40, Issue 4
DOI: 10.1021/bi001641+

Identification of a novel quinone-binding site in the cytochrome bo complex from Escherichia coli.
journal, November 1994

Sato-Watanabe, M.; Mogi, T.; Ogura, T.
Journal of Biological Chemistry, Vol. 269, Issue 46
DOI: 10.1016/S0021-9258(19)61993-9

Transient Formation of Ubisemiquinone Radical and Subsequent Electron Transfer Process in the Escherichia coli Cytochrome bo ^†
journal, December 2000

Kobayashi, Kazuo; Tagawa, Seiichi; Mogi, Tatsushi
Biochemistry, Vol. 39, Issue 50
DOI: 10.1021/bi0014094

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents*
journal, March 2011

Lin, Myat T.; Shubin, Alexander A.; Samoilova, Rimma I.
Journal of Biological Chemistry, Vol. 286, Issue 12
DOI: 10.1074/jbc.M110.206821

Characterization of the Exchangeable Protons in the Immediate Vicinity of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli
journal, June 2006

Yap, Lai Lai; Samoilova, Rimma I.; Gennis, Robert B.
Journal of Biological Chemistry, Vol. 281, Issue 25
DOI: 10.1074/jbc.M602544200

Similar Records in DOE PAGES and OSTI.GOV collections:

Cryo-EM structures of Escherichia coli cytochrome bo₃ reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site

Journal Article Li, Jiao ; Han, Long ; Vallese, Francesca ; ... - Proceedings of the National Academy of Sciences of the United States of America

Two independent structures of the proton-pumping, respiratory cytochrome bo3 ubiquinol oxidase (cyt bo₃) have been determined by cryogenic electron microscopy (cryo-EM) in styrene–maleic acid (SMA) copolymer nanodiscs and in membrane scaffold protein (MSP) nanodiscs to 2.55- and 2.19-Å resolution, respectively. The structures include the metal redox centers (heme b, heme o₃, and Cu_B), the redox-active cross-linked histidine–tyrosine cofactor, and the internal water molecules in the proton-conducting D channel. Each structure also contains one equivalent of ubiquinone-8 (UQ8) in the substrate binding site as well as several phospholipid molecules. The isoprene side chain of UQ8 is clamped within a hydrophobic groovemore »« less
https://doi.org/10.1073/pnas.2106750118

Full Text Available
The Ubiquinol Binding Site of Cytochrome bo ₃ from Escherichia coli Accommodates Menaquinone and Stabilizes a Functional Menasemiquinone

Journal Article Ding, Ziqiao ; Sun, Chang ; Yi, Sophia M. ; ... - Biochemistry

Not provided.
https://doi.org/10.1021/acs.biochem.9b00750
Differential effects of glutamate-286 mutations in the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides and the cytochrome bo3 ubiquinol oxidase from Escherichia coli

Journal Article Egawa, Tsuyoshi ; Ganesan, Krithika ; Lin, Myat T. ; ... - Biochimica et Biophysica Acta - Bioenergetics
Cited by 2
https://doi.org/10.1016/j.bbabio.2011.06.001
Structural Basis of Low-Affinity Nickel Binding to the Nickel-Responsive Transcription Factor NikR from Escherichia coli

Journal Article Phillips, C ; Schreiter, E ; Stultz, C ; ... - Biochemistry

Escherichia coli NikR regulates cellular nickel uptake by binding to the nik operon in the presence of nickel and blocking transcription of genes encoding the nickel uptake transporter. NikR has two binding affinities for the nik operon: a nanomolar dissociation constant with stoichiometric nickel and a picomolar dissociation constant with excess nickel [Bloom, S. L., and Zamble, D. B. (2004) Biochemistry 43, 10029-10038; Chivers, P. T., and Sauer, R. T. (2002) Chem. Biol. 9, 1141-1148]. While it is known that the stoichiometric nickel ions bind at the NikR tetrameric interface [Schreiter, E. R., et al. (2003) Nat. Struct. Biol. 10,more »« less
https://doi.org/10.1021/bi100923j
Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase

Journal Article Koland, J G ; Miller, M J ; Gennis, R B - Biochemistry; (United States)

Pyruvate oxidase is a flavoprotein dehydrogenase located on the inner surface of the Escherichia coli cytoplasmic membrane and coupled to the E. coli aerobic respiratory chain. The role of quinones in the pyruvate oxidase system is investigated, and a minimal respiratory chain is described consisting of only two pure proteins plus ubiquinone 8 incorporated in phospholipid vesicles. The enzymes used in this reconstitution are the flavorprotein and the recently purified E. coli cytochrome d terminal oxidase. The catalytic velocity of the reconstituted liposome system is about 30% of that observed when the flavoprotein is reconstituted with E. coli membranes. Itmore »« less
https://doi.org/10.1021/bi00298a008

Similar Records

Title: Searching for the low affinity ubiquinone binding site in cytochrome bo3 from Escherichia coli

Citation Formats

Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase journal, October 2006

Characterization of the Ubiquinol Oxidation Sites in Cytochromes bo and bd from Escherichia coli using Aurachin C Analogues journal, January 1999

The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli journal, December 2010

On the function of the various quinone species in Escherichia coli : The role of DMK in the electron transfer chains of journal, May 2012

Studies on a Stabilisation of Ubisemiquinone by Escherichia coli Quinol Oxidase, Cytochrome bo journal, February 1995

Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry To Map the Quinol Binding Site of Cytochrome bo 3 from Escherichia coli † journal, July 1998

Identification of the Residues Involved in Stabilization of the Semiquinone Radical in the High-Affinity Ubiquinone Binding Site in Cytochrome bo 3 from Escherichia coli by Site-Directed Mutagenesis and EPR Spectroscopy † journal, August 2002

Vibrational Modes of Ubiquinone in Cytochrome bo 3 from Escherichia coli Identified by Fourier Transform Infrared Difference Spectroscopy and Specific 13 C Labeling † journal, November 1999

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q H Site of Wild-Type and D75H Mutant Cytochrome bo 3 from Escherichia coli journal, April 2012

Asymmetric Binding of the High-Affinity Q H • - Ubisemiquinone in Quinol Oxidase ( bo 3 ) from Escherichia coli Studied by Multifrequency Electron Paramagnetic Resonance Spectroscopy † journal, May 2003

Reconstitution of the Ubiquinone-dependent pyruvate oxidase system of Escherichia coli with the cytochrome o terminal oxidase complex. journal, September 1985

Cytochrome o ( bo ) is a proton pump in Paracoccus denitrificans and Escherichia coli journal, June 1989

A study of cytochrome bo3 in a tethered bilayer lipid membrane journal, December 2010

Tryptophan-136 in Subunit II of Cytochrome bo 3 from Escherichia c oli May Participate in the Binding of Ubiquinol † journal, August 1998

Direct Evidence for the Protonation of Aspartate-75, Proposed To Be at a Quinol Binding Site, upon Reduction of Cytochrome bo 3 from Escherichia coli † journal, January 2001

Protein−Cofactor Interactions and EPR Parameters for the Q H Quinone Binding Site of Quinol Oxidase. A Density Functional Study journal, May 2006

Mimicking respiratory phosphorylation using purified enzymes journal, April 2016

The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases. journal, July 1990

The superfamily of heme–copper oxygen reductases: Types and evolutionary considerations journal, April 2012

A Bioinformatics Classifier and Database for Heme-Copper Oxygen Reductases journal, April 2011

Monitoring the Transmembrane Proton Gradient Generated by Cytochrome bo 3 in Tethered Bilayer Lipid Membranes Using SEIRA Spectroscopy journal, February 2016

Identification of the Nitrogen Donor Hydrogen Bonded with the Semiquinone at the Q H Site of the Cytochrome bo 3 from Escherichia coli journal, November 2008

An Elementary Reaction Step of the Proton Pump Is Revealed by Mutation of Tryptophan-164 to Phenylalanine in Cytochrome c Oxidase from Paracoccus denitrificans † journal, December 2005

ENDOR spectroscopic studies of stable semiquinone radicals bound to the Escherichia coli cytochrome bo3 quinol oxidase: ENDOR of cytochrome bo3 semiquinones journal, September 2000

Isolation and Characterizations of Quinone Analogue-Resistant Mutants of b o -Type Ubiquinol Oxidase from Escherichia c oli † journal, September 1998

Q-Band ENDOR (Electron Nuclear Double Resonance) of the High-Affinity Ubisemiquinone Center in Cytochrome bo 3 from Escherichia coli † journal, March 2000

Prediction of high- and low-affinity quinol-analogue-binding sites in the aa3 and bo3 terminal oxidases from Bacillus subtilis and Escherichia coli1 journal, June 2014

Subunit II of the Cytochrome bo 3 Ubiquinol Oxidase from Escherichia coli Is a Lipoprotein journal, September 1997

Stabilization of a semiquinone radical at the high-affinity quinone-binding site (Q H ) of the Escherichia coli bo -type ubiquinol oxidase journal, October 1995

Role of a bound ubiquinone on reactions of the Escherichia coli cytochrome bo with ubiquinol and dioxygen journal, August 1999

Characterization of Mutants That Change the Hydrogen Bonding of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli* journal, March 2007

Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols. journal, November 1994

QH•- Ubisemiquinone Radical in the bo3-Type Ubiquinol Oxidase Studied by Pulsed Electron Paramagnetic Resonance and Hyperfine Sublevel Correlation Spectroscopy journal, January 2001

Identification of a novel quinone-binding site in the cytochrome bo complex from Escherichia coli. journal, November 1994

Transient Formation of Ubisemiquinone Radical and Subsequent Electron Transfer Process in the Escherichia coli Cytochrome bo † journal, December 2000

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents* journal, March 2011

Characterization of the Exchangeable Protons in the Immediate Vicinity of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli journal, June 2006

Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase
journal, October 2006

Characterization of the Ubiquinol Oxidation Sites in Cytochromes bo and bd from Escherichia coli using Aurachin C Analogues
journal, January 1999

The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli
journal, December 2010

On the function of the various quinone species in Escherichia coli : The role of DMK in the electron transfer chains of
journal, May 2012

Studies on a Stabilisation of Ubisemiquinone by Escherichia coli Quinol Oxidase, Cytochrome bo
journal, February 1995

Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry To Map the Quinol Binding Site of Cytochrome bo ₃ from Escherichia coli ^†
journal, July 1998

Identification of the Residues Involved in Stabilization of the Semiquinone Radical in the High-Affinity Ubiquinone Binding Site in Cytochrome bo ₃ from Escherichia coli by Site-Directed Mutagenesis and EPR Spectroscopy ^†
journal, August 2002

Vibrational Modes of Ubiquinone in Cytochrome bo ₃ from Escherichia coli Identified by Fourier Transform Infrared Difference Spectroscopy and Specific ¹³ C Labeling ^†
journal, November 1999

Interactions of Intermediate Semiquinone with Surrounding Protein Residues at the Q _H Site of Wild-Type and D75H Mutant Cytochrome bo ₃ from Escherichia coli
journal, April 2012

Asymmetric Binding of the High-Affinity Q _H ^• ^- Ubisemiquinone in Quinol Oxidase ( bo ₃ ) from Escherichia coli Studied by Multifrequency Electron Paramagnetic Resonance Spectroscopy ^†
journal, May 2003

Reconstitution of the Ubiquinone-dependent pyruvate oxidase system of Escherichia coli with the cytochrome o terminal oxidase complex.
journal, September 1985

Cytochrome o ( bo ) is a proton pump in Paracoccus denitrificans and Escherichia coli
journal, June 1989

A study of cytochrome bo3 in a tethered bilayer lipid membrane
journal, December 2010

Tryptophan-136 in Subunit II of Cytochrome bo ₃ from Escherichia c oli May Participate in the Binding of Ubiquinol ^†
journal, August 1998

Direct Evidence for the Protonation of Aspartate-75, Proposed To Be at a Quinol Binding Site, upon Reduction of Cytochrome bo ₃ from Escherichia coli ^†
journal, January 2001

Protein−Cofactor Interactions and EPR Parameters for the Q _H Quinone Binding Site of Quinol Oxidase. A Density Functional Study
journal, May 2006

Mimicking respiratory phosphorylation using purified enzymes
journal, April 2016

The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases.
journal, July 1990

The superfamily of heme–copper oxygen reductases: Types and evolutionary considerations
journal, April 2012

A Bioinformatics Classifier and Database for Heme-Copper Oxygen Reductases
journal, April 2011

Monitoring the Transmembrane Proton Gradient Generated by Cytochrome bo ₃ in Tethered Bilayer Lipid Membranes Using SEIRA Spectroscopy
journal, February 2016

Identification of the Nitrogen Donor Hydrogen Bonded with the Semiquinone at the Q _H Site of the Cytochrome bo ₃ from Escherichia coli
journal, November 2008

An Elementary Reaction Step of the Proton Pump Is Revealed by Mutation of Tryptophan-164 to Phenylalanine in Cytochrome c Oxidase from Paracoccus denitrificans ^†
journal, December 2005

ENDOR spectroscopic studies of stable semiquinone radicals bound to the Escherichia coli cytochrome bo3 quinol oxidase: ENDOR of cytochrome bo3 semiquinones
journal, September 2000

Isolation and Characterizations of Quinone Analogue-Resistant Mutants of b o -Type Ubiquinol Oxidase from Escherichia c oli ^†
journal, September 1998

Q-Band ENDOR (Electron Nuclear Double Resonance) of the High-Affinity Ubisemiquinone Center in Cytochrome bo ₃ from Escherichia coli ^†
journal, March 2000

Prediction of high- and low-affinity quinol-analogue-binding sites in the aa3 and bo3 terminal oxidases from Bacillus subtilis and Escherichia coli1
journal, June 2014

Subunit II of the Cytochrome bo ₃ Ubiquinol Oxidase from Escherichia coli Is a Lipoprotein
journal, September 1997

Stabilization of a semiquinone radical at the high-affinity quinone-binding site (Q _H ) of the Escherichia coli bo -type ubiquinol oxidase
journal, October 1995

Role of a bound ubiquinone on reactions of the Escherichia coli cytochrome bo with ubiquinol and dioxygen
journal, August 1999

Characterization of Mutants That Change the Hydrogen Bonding of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli*
journal, March 2007

Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols.
journal, November 1994

Q_H^•- Ubisemiquinone Radical in the bo₃-Type Ubiquinol Oxidase Studied by Pulsed Electron Paramagnetic Resonance and Hyperfine Sublevel Correlation Spectroscopy
journal, January 2001

Identification of a novel quinone-binding site in the cytochrome bo complex from Escherichia coli.
journal, November 1994

Transient Formation of Ubisemiquinone Radical and Subsequent Electron Transfer Process in the Escherichia coli Cytochrome bo ^†
journal, December 2000

Exploring by Pulsed EPR the Electronic Structure of Ubisemiquinone Bound at the QH Site of Cytochrome bo3 from Escherichia coli with in Vivo 13C-Labeled Methyl and Methoxy Substituents*
journal, March 2011

Characterization of the Exchangeable Protons in the Immediate Vicinity of the Semiquinone Radical at the QH Site of the Cytochrome bo3 from Escherichia coli
journal, June 2006