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Title: Photosystem I-LHCII megacomplexes respond to high light and aging in plants

Abstract

Photosystem II is known to be a highly dynamic multi-protein complex that participates in a variety of regulatory and repair processes. In contrast, photosystem I (PSI) has, until quite recently, been thought of as relatively static. We report the discovery of plant PSI-LHCII megacomplexes containing multiple LHCII trimers per PSI reaction center. These PSI-LHCII megacomplexes respond rapidly to changes in light intensity, as visualized by native gel electrophoresis. PSI-LHCII megacomplex formation was found to require thylakoid stacking, and to depend upon growth light intensity and leaf age. These factors were, in turn, correlated with changes in PSI/PSII ratios and, intriguingly, PSI-LHCII megacomplex dynamics appeared to depend upon PSII core phosphorylation. Thus, these findings suggest new functions for PSI and a new level of regulation involving specialized subpopulations of photosystem I which have profound implications for current models of thylakoid dynamics.

Authors:
 [1];  [1];  [1]
  1. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Washington State Univ., Pullman, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1396408
Alternate Identifier(s):
OSTI ID: 1501434
Grant/Contract Number:  
FG02-04ER15559; FG02-04ER 15559
Resource Type:
Published Article
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 136; Journal Issue: 1; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Photosystem I; Photosystem II; LHC; Complex; Supercomplex; Megacomplex; High light; Senescence; PsaH; Native gel; Green gel

Citation Formats

Schwarz, Eliezer M., Tietz, Stephanie, and Froehlich, John E. Photosystem I-LHCII megacomplexes respond to high light and aging in plants. United States: N. p., 2017. Web. doi:10.1007/s11120-017-0447-y.
Schwarz, Eliezer M., Tietz, Stephanie, & Froehlich, John E. Photosystem I-LHCII megacomplexes respond to high light and aging in plants. United States. doi:10.1007/s11120-017-0447-y.
Schwarz, Eliezer M., Tietz, Stephanie, and Froehlich, John E. Tue . "Photosystem I-LHCII megacomplexes respond to high light and aging in plants". United States. doi:10.1007/s11120-017-0447-y.
@article{osti_1396408,
title = {Photosystem I-LHCII megacomplexes respond to high light and aging in plants},
author = {Schwarz, Eliezer M. and Tietz, Stephanie and Froehlich, John E.},
abstractNote = {Photosystem II is known to be a highly dynamic multi-protein complex that participates in a variety of regulatory and repair processes. In contrast, photosystem I (PSI) has, until quite recently, been thought of as relatively static. We report the discovery of plant PSI-LHCII megacomplexes containing multiple LHCII trimers per PSI reaction center. These PSI-LHCII megacomplexes respond rapidly to changes in light intensity, as visualized by native gel electrophoresis. PSI-LHCII megacomplex formation was found to require thylakoid stacking, and to depend upon growth light intensity and leaf age. These factors were, in turn, correlated with changes in PSI/PSII ratios and, intriguingly, PSI-LHCII megacomplex dynamics appeared to depend upon PSII core phosphorylation. Thus, these findings suggest new functions for PSI and a new level of regulation involving specialized subpopulations of photosystem I which have profound implications for current models of thylakoid dynamics.},
doi = {10.1007/s11120-017-0447-y},
journal = {Photosynthesis Research},
number = 1,
volume = 136,
place = {United States},
year = {2017},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1007/s11120-017-0447-y

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Cited by: 3 works
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Works referenced in this record:

Photosystem II core phosphorylation and photosynthetic acclimation require two different protein kinases
journal, October 2005

  • Bonardi, Vera; Pesaresi, Paolo; Becker, Thomas
  • Nature, Vol. 437, Issue 7062
  • DOI: 10.1038/nature04016

Arabidopsis STN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation
journal, August 2009

  • Pesaresi, Paolo; Hertle, Alexander; Pribil, Mathias
  • The Plant Cell, Vol. 21, Issue 8
  • DOI: 10.1105/tpc.108.064964

Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize
journal, October 2009

  • Pokorska, Berenika; Zienkiewicz, Maksymilian; Powikrowska, Marta
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1787, Issue 10
  • DOI: 10.1016/j.bbabio.2009.05.002

Molecular and Global Time-resolved Analysis of a psbS Gene Dosage Effect on pH- and Xanthophyll Cycle-dependent Nonphotochemical Quenching in Photosystem II
journal, July 2002

  • Li, Xiao-Ping; Gilmore, Adam M.; Niyogi, Krishna K.
  • Journal of Biological Chemistry, Vol. 277, Issue 37
  • DOI: 10.1074/jbc.M204797200

Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature
journal, July 2013


Architectural switch in plant photosynthetic membranes induced by light stress
journal, November 2012

  • Herbstova, M.; Tietz, S.; Kinzel, C.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 49
  • DOI: 10.1073/pnas.1214265109

Possible effects of the detachment of stromal lamellae from granal stacks on salt-induced changes in spillover. A study by sonication of chloroplasts
journal, April 1981

  • Chow, W. S.; Ford, R. C.; Barber, J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 635, Issue 2
  • DOI: 10.1016/0005-2728(81)90030-X

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
journal, August 1970


The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis
journal, November 2000

  • Lunde, Christina; Jensen, Poul Erik; Haldrup, Anna
  • Nature, Vol. 408, Issue 6812
  • DOI: 10.1038/35046121

Compartmentalization of the protein repair machinery in photosynthetic membranes
journal, October 2014

  • Puthiyaveetil, S.; Tsabari, O.; Lowry, T.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 44
  • DOI: 10.1073/pnas.1413739111

Structure and dynamics of thylakoids in land plants
journal, March 2014

  • Pribil, Mathias; Labs, Mathias; Leister, Dario
  • Journal of Experimental Botany, Vol. 65, Issue 8
  • DOI: 10.1093/jxb/eru090

Spatial Organization and Interaction of the Two Photosystems in Photosynthesis
journal, March 1970


The Chloroplast NAD(P)H Dehydrogenase Complex Interacts with Photosystem I in Arabidopsis
journal, October 2008

  • Peng, Lianwei; Shimizu, Hideyuki; Shikanai, Toshiharu
  • Journal of Biological Chemistry, Vol. 283, Issue 50
  • DOI: 10.1074/jbc.M803207200

AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability
journal, October 2013

  • Seok, Min Sook; You, Young Nim; Park, Hyun Ji
  • Physiologia Plantarum, Vol. 150, Issue 4
  • DOI: 10.1111/ppl.12116

Close Relationships Between the PSII Repair Cycle and Thylakoid Membrane Dynamics
journal, March 2016

  • Yoshioka-Nishimura, Miho
  • Plant and Cell Physiology, Vol. 57, Issue 6
  • DOI: 10.1093/pcp/pcw050

The structure of plant photosystem I super-complex at 2.8 Å resolution
journal, June 2015


A megacomplex composed of both photosystem reaction centres in higher plants
journal, March 2015

  • Yokono, M.; Takabayashi, A.; Akimoto, S.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7675

Resolution of 16 to 20 chlorophyII-protein complexes using a low lonic strength native green gel system
journal, April 1991


Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery — including both photosystems II and I
journal, June 2015

  • Grieco, Michele; Suorsa, Marjaana; Jajoo, Anjana
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1847, Issue 6-7
  • DOI: 10.1016/j.bbabio.2015.03.004

Excitation energy transfer between Light-harvesting complex II and Photosystem I in reconstituted membranes
journal, April 2016

  • Akhtar, Parveen; Lingvay, Mónika; Kiss, Teréz
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 4
  • DOI: 10.1016/j.bbabio.2016.01.016

High-light vs. low-light: Effect of light acclimation on photosystem II composition and organization in Arabidopsis thaliana
journal, March 2013

  • Kouřil, Roman; Wientjes, Emilie; Bultema, Jelle B.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 3
  • DOI: 10.1016/j.bbabio.2012.12.003

Why do thylakoid membranes from higher plants form grana stacks?
journal, November 1993


Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light
journal, November 2008

  • Tikkanen, Mikko; Nurmi, Markus; Kangasjärvi, Saijaliisa
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1777, Issue 11
  • DOI: 10.1016/j.bbabio.2008.08.004

Changes in the lateral distribution of the light-harvesting chlorophyll-a/b-protein complex induced by its phosphorylation
journal, October 1983


Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy
journal, March 2007

  • van der Weij-de Wit, C. D.; Ihalainen, J. A.; van Grondelle, R.
  • Photosynthesis Research, Vol. 93, Issue 1-3
  • DOI: 10.1007/s11120-007-9157-1

Dynamic flexibility in the structure and function of photosystem II in higher plant thylakoid membranes: the grana enigma
journal, October 2008

  • Anderson, Jan M.; Chow, Wah Soon; De Las Rivas, Javier
  • Photosynthesis Research, Vol. 98, Issue 1-3
  • DOI: 10.1007/s11120-008-9381-3

Anomalous electron transport activity in a Photosystem I-deficient maize mutant
journal, April 1990

  • Cook, William B.; Miles, Donald
  • Photosynthesis Research, Vol. 24, Issue 1
  • DOI: 10.1007/BF00032647

Photosynthetic Energy Conversion Efficiency: Setting a Baseline for Gauging Future Improvements in Important Food and Biofuel Crops
journal, March 2015

  • Slattery, Rebecca A.; Ort, Donald R.
  • Plant Physiology, Vol. 168, Issue 2
  • DOI: 10.1104/pp.15.00066

Supramolecular organization of thylakoid membrane proteins in green plants
journal, January 2005

  • Dekker, Jan P.; Boekema, Egbert J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1706, Issue 1-2
  • DOI: 10.1016/j.bbabio.2004.09.009

Efficient Light Harvesting by Photosystem II Requires an Optimized Protein Packing Density in Grana Thylakoids
journal, April 2010

  • Haferkamp, Silvia; Haase, Winfried; Pascal, Andrew A.
  • Journal of Biological Chemistry, Vol. 285, Issue 22
  • DOI: 10.1074/jbc.M109.077750

Improving Photosynthetic Efficiency for Greater Yield
journal, June 2010


Redesigning photosynthesis to sustainably meet global food and bioenergy demand
journal, June 2015

  • Ort, Donald R.; Merchant, Sabeeha S.; Alric, Jean
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 28
  • DOI: 10.1073/pnas.1424031112

Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants
journal, May 2008

  • Tikkanen, Mikko; Nurmi, Markus; Suorsa, Marjaana
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1777, Issue 5
  • DOI: 10.1016/j.bbabio.2008.02.001

A pigment-binding protein essential for regulation of photosynthetic light harvesting
journal, January 2000

  • Li, Xiao-Ping; Björkman, Olle; Shih, Connie
  • Nature, Vol. 403, Issue 6768
  • DOI: 10.1038/35000131

Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy
journal, January 2016

  • Albanese, Pascal; Nield, Jon; Tabares, Jose Alejandro Muñoz
  • Photosynthesis Research, Vol. 130, Issue 1-3
  • DOI: 10.1007/s11120-016-0216-3

Role of subunits in eukaryotic Photosystem I
journal, October 2001

  • Scheller, Henrik Vibe; Jensen, Poul Erik; Haldrup, Anna
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1507, Issue 1-3
  • DOI: 10.1016/S0005-2728(01)00196-7

PGR5 Is Involved in Cyclic Electron Flow around Photosystem I and Is Essential for Photoprotection in Arabidopsis
journal, August 2002


Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes
journal, September 2011

  • Järvi, Sari; Suorsa, Marjaana; Paakkarinen, Virpi
  • Biochemical Journal, Vol. 439, Issue 2
  • DOI: 10.1042/BJ20102155

Granal stacking of thylakoid membranes in higher plant chloroplasts: the physicochemical forces at work and the functional consequences that ensue
journal, January 2005

  • Chow, Wah Soon; Kim, Eun-Ha; Horton, Peter
  • Photochemical & Photobiological Sciences, Vol. 4, Issue 12
  • DOI: 10.1039/b507310n

Lateral heterogeneity in the distribution of chlorophyll-protein complexes of the thylakoid membranes of spinach chloroplasts
journal, December 1980

  • Andersson, Bertil; Anderson, Jan M.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 593, Issue 2
  • DOI: 10.1016/0005-2728(80)90078-X

Effects of cations upon chloroplast membrane subunit interactions and excitation energy distribution
journal, November 1976


Antenna Size Dependency of Photoinactivation of Photosystem II in Light-Acclimated Pea Leaves
journal, September 1997

  • Park II, Y.; Chow, W. S.; Anderson, J. M.
  • Plant Physiology, Vol. 115, Issue 1
  • DOI: 10.1104/pp.115.1.151

Light acclimation involves dynamic re-organization of the pigment-protein megacomplexes in non-appressed thylakoid domains
journal, October 2015

  • Suorsa, Marjaana; Rantala, Marjaana; Mamedov, Fikret
  • The Plant Journal, Vol. 84, Issue 2
  • DOI: 10.1111/tpj.13004

Structural changes of the thylakoid membrane network induced by high light stress in plant chloroplasts
journal, April 2014

  • Kirchhoff, Helmut
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1640
  • DOI: 10.1098/rstb.2013.0225

Plants Impaired in State Transitions Can to a Large Degree Compensate for their Defect
journal, January 2003

  • Lunde, Christina; Jensen, Poul Erik; Rosgaard, Lisa
  • Plant and Cell Physiology, Vol. 44, Issue 1
  • DOI: 10.1093/pcp/pcg012

Simulation of grana stacking in a model membrane system. Mediation by a purified light-harvesting pigment-protein complex from chloroplasts
journal, April 1980

  • Mullet, John E.; Arntzen, Charles J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 589, Issue 1
  • DOI: 10.1016/0005-2728(80)90135-8

Phosphorylation of Photosystem II Controls Functional Macroscopic Folding of Photosynthetic Membranes in Arabidopsis
journal, December 2009

  • Fristedt, Rikard; Willig, Adrian; Granath, Pontus
  • The Plant Cell, Vol. 21, Issue 12
  • DOI: 10.1105/tpc.109.069435

Photosynthetic response to fluctuating environments and photoprotective strategies under abiotic stress
journal, March 2016


Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis
journal, April 2010

  • Iwai, Masakazu; Takizawa, Kenji; Tokutsu, Ryutaro
  • Nature, Vol. 464, Issue 7292
  • DOI: 10.1038/nature08885

Plants Actively Avoid State Transitions upon Changes in Light Intensity: Role of Light-Harvesting Complex II Protein Dephosphorylation in High Light
journal, April 2015

  • Mekala, Nageswara Rao; Suorsa, Marjaana; Rantala, Marjaana
  • Plant Physiology, Vol. 168, Issue 2
  • DOI: 10.1104/pp.15.00488

STN8 Protein Kinase in Arabidopsis thaliana Is Specific in Phosphorylation of Photosystem II Core Proteins
journal, July 2005

  • Vainonen, Julia P.; Hansson, Maria; Vener, Alexander V.
  • Journal of Biological Chemistry, Vol. 280, Issue 39
  • DOI: 10.1074/jbc.M505729200

Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions
journal, October 2015


Activation of the Stt7/STN7 Kinase through Dynamic Interactions with the Cytochrome b 6 f Complex
journal, March 2016

  • Shapiguzov, Alexey; Chai, Xin; Fucile, Geoffrey
  • Plant Physiology, Vol. 171, Issue 1
  • DOI: 10.1104/pp.15.01893

Photosystem I: a search for green plant trimers
journal, January 2005

  • Kouřil, Roman; van Oosterwijk, Niels; Yakushevska, Alevtyna E.
  • Photochemical & Photobiological Sciences, Vol. 4, Issue 12
  • DOI: 10.1039/b505519a

A Protein Phosphorylation Threshold for Functional Stacking of Plant Photosynthetic Membranes
journal, June 2010


Structural constraints for protein repair in plant photosynthetic membranes
journal, April 2013

  • Kirchhoff, Helmut
  • Plant Signaling & Behavior, Vol. 8, Issue 4
  • DOI: 10.4161/psb.23634

The Light-Harvesting Chlorophyll a/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis
journal, September 2014

  • Pietrzykowska, M.; Suorsa, M.; Semchonok, D. A.
  • The Plant Cell, Vol. 26, Issue 9
  • DOI: 10.1105/tpc.114.127373

Chlorophyll-protein complexes of barley photosystem I
journal, March 1987


Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential
journal, March 2015


The stacking of chloroplast thylakoids
journal, April 1980


Age-dependent changes in the functions and compositions of photosynthetic complexes in the thylakoid membranes of Arabidopsis thaliana
journal, August 2013


State transitions and light adaptation require chloroplast thylakoid protein kinase STN7
journal, February 2005

  • Bellafiore, Stéphane; Barneche, Frédy; Peltier, Gilles
  • Nature, Vol. 433, Issue 7028
  • DOI: 10.1038/nature03286

Quality Control of PSII: Behavior of PSII in the Highly Crowded Grana Thylakoids Under Excessive Light
journal, March 2014

  • Yamamoto, Yasusi; Kai, Suguru; Ohnishi, Atsuki
  • Plant and Cell Physiology, Vol. 55, Issue 7
  • DOI: 10.1093/pcp/pcu043