Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids

Wilson, Robert L.; Frisz, Jessica F.; Klitzing, Haley A.; Zimmerberg, Joshua; Weber, Peter K.; Kraft, Mary L.

doi:10.1016/j.bpj.2015.02.026

Title: Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids

Abstract

The clusters of the influenza envelope protein, hemagglutinin, within the plasma membrane are hypothesized to be enriched with cholesterol and sphingolipids. Here in this paper, we directly tested this hypothesis by using high-resolution secondary ion mass spectrometry to image the distributions of antibody-labeled hemagglutinin and isotope-labeled cholesterol and sphingolipids in the plasma membranes of fibroblast cells that stably express hemagglutinin. We found that the hemagglutinin clusters were neither enriched with cholesterol nor colocalized with sphingolipid domains. Thus, hemagglutinin clustering and localization in the plasma membrane is not controlled by cohesive interactions between hemagglutinin and liquid-ordered domains enriched with cholesterol and sphingolipids, or from specific binding interactions between hemagglutinin, cholesterol, and/or the majority of sphingolipid species in the plasma membrane.

Authors:: Wilson, Robert L.; Frisz, Jessica F.; Klitzing, Haley A.; Zimmerberg, Joshua; Weber, Peter K.; Kraft, Mary L.

Publication Date:: Wed Apr 01 00:00:00 EDT 2015

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE; National Science Foundation (NSF); National Institutes of Health (NIH)

OSTI Identifier:: 1246558

Alternate Identifier(s):: OSTI ID: 1396214

Report Number(s):: LLNL-JRNL-698175
Journal ID: ISSN 0006-3495; S0006349515002246; PII: S0006349515002246

Grant/Contract Number:: AC52-07NA27344

Resource Type:: Published Article

Journal Name:: Biophysical Journal

Additional Journal Information:: Journal Name: Biophysical Journal Journal Volume: 108 Journal Issue: 7; Journal ID: ISSN 0006-3495

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY

Citation Formats


                    Wilson, Robert L., Frisz, Jessica F., Klitzing, Haley A., Zimmerberg, Joshua, Weber, Peter K., and Kraft, Mary L. Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids.  United States: N. p., 2015. 
Web.  doi:10.1016/j.bpj.2015.02.026.

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                    Wilson, Robert L., Frisz, Jessica F., Klitzing, Haley A., Zimmerberg, Joshua, Weber, Peter K., & Kraft, Mary L. Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids.  United States.  https://doi.org/10.1016/j.bpj.2015.02.026

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                    Wilson, Robert L., Frisz, Jessica F., Klitzing, Haley A., Zimmerberg, Joshua, Weber, Peter K., and Kraft, Mary L. Wed .  
"Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids".  United States.  https://doi.org/10.1016/j.bpj.2015.02.026.

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@article{osti_1246558,

  title        = {Hemagglutinin Clusters in the Plasma Membrane Are Not Enriched with Cholesterol and Sphingolipids},

  author       = {Wilson, Robert L. and Frisz, Jessica F. and Klitzing, Haley A. and Zimmerberg, Joshua and Weber, Peter K. and Kraft, Mary L.},

  abstractNote = {The clusters of the influenza envelope protein, hemagglutinin, within the plasma membrane are hypothesized to be enriched with cholesterol and sphingolipids. Here in this paper, we directly tested this hypothesis by using high-resolution secondary ion mass spectrometry to image the distributions of antibody-labeled hemagglutinin and isotope-labeled cholesterol and sphingolipids in the plasma membranes of fibroblast cells that stably express hemagglutinin. We found that the hemagglutinin clusters were neither enriched with cholesterol nor colocalized with sphingolipid domains. Thus, hemagglutinin clustering and localization in the plasma membrane is not controlled by cohesive interactions between hemagglutinin and liquid-ordered domains enriched with cholesterol and sphingolipids, or from specific binding interactions between hemagglutinin, cholesterol, and/or the majority of sphingolipid species in the plasma membrane.},

  doi          = {10.1016/j.bpj.2015.02.026},

  journal      = {Biophysical Journal},

  number       = 7,

  volume       = 108,

  place        = {United States},

  year         = {Wed Apr 01 00:00:00 EDT 2015},

  month        = {Wed Apr 01 00:00:00 EDT 2015}

}

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Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs
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