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Title: The Gut of Geographically Disparate Ciona intestinalis Harbors a Core Microbiota

It is now widely understood that all animals engage in complex interactions with bacteria (or microbes) throughout their various life stages. This ancient exchange can involve cooperation and has resulted in a wide range of evolved host-microbial interdependencies, including those observed in the gut. Ciona intestinalis, a filter-feeding basal chordate and classic developmental model that can be experimentally manipulated, is being employed to help define these relationships. Ciona larvae are first exposed internally to microbes upon the initiation of feeding in metamorphosed individuals; however, whether or not these microbes subsequently colonize the gut and whether or not Ciona forms relationships with specific bacteria in the gut remains unknown. Here in this report, we show that the Ciona gut not only is colonized by a complex community of bacteria, but also that samples from three geographically isolated populations reveal striking similarity in abundant operational taxonomic units (OTUs) consistent with the selection of a core community by the gut ecosystem.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [6] ;  [6] ;  [6] ;  [6] ;  [1] ;  [8]
  1. Univ. of South Florida, St. Petersburg, FL (United States). College of Medicine, Division of Molecular Genetics, Dept. of Pediatrics
  2. Univ. of South Florida, St. Petersburg, FL (United States). College of Medicine, Division of Neonatology
  3. Univ. of Chicago, IL (United States). Dept. of Ecology and Evolution
  4. Univ. of South Florida, St. Petersburg, FL (United States). College of Medicine
  5. Univ. of South Florida, St. Petersburg, FL (United States). College of Marine Sciences
  6. Stazione Zoologica Anton Dohrn (SZN), Dohrn, Naples (Italy). Dept. of Animal Physiology and Evolution
  7. All Children's Hospital, St. Petersburg, FL (United States). Dept. of Molecular Genetics
  8. Univ. of Chicago, IL (United States). Dept. of Ecology and Evolution; Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 9; Journal Issue: 4; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE; National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
OSTI Identifier:
1396034

Dishaw, Larry J., Flores-Torres, Jaime, Lax, Simon, Gemayel, Kristina, Leigh, Brittany, Melillo, Daniela, Mueller, M. Gail, Natale, Lenina, Zucchetti, Ivana, De Santis, Rosaria, Pinto, Maria Rosaria, Litman, Gary W., and Gilbert, Jack A.. The Gut of Geographically Disparate Ciona intestinalis Harbors a Core Microbiota. United States: N. p., Web. doi:10.1371/journal.pone.0093386.
Dishaw, Larry J., Flores-Torres, Jaime, Lax, Simon, Gemayel, Kristina, Leigh, Brittany, Melillo, Daniela, Mueller, M. Gail, Natale, Lenina, Zucchetti, Ivana, De Santis, Rosaria, Pinto, Maria Rosaria, Litman, Gary W., & Gilbert, Jack A.. The Gut of Geographically Disparate Ciona intestinalis Harbors a Core Microbiota. United States. doi:10.1371/journal.pone.0093386.
Dishaw, Larry J., Flores-Torres, Jaime, Lax, Simon, Gemayel, Kristina, Leigh, Brittany, Melillo, Daniela, Mueller, M. Gail, Natale, Lenina, Zucchetti, Ivana, De Santis, Rosaria, Pinto, Maria Rosaria, Litman, Gary W., and Gilbert, Jack A.. 2014. "The Gut of Geographically Disparate Ciona intestinalis Harbors a Core Microbiota". United States. doi:10.1371/journal.pone.0093386. https://www.osti.gov/servlets/purl/1396034.
@article{osti_1396034,
title = {The Gut of Geographically Disparate Ciona intestinalis Harbors a Core Microbiota},
author = {Dishaw, Larry J. and Flores-Torres, Jaime and Lax, Simon and Gemayel, Kristina and Leigh, Brittany and Melillo, Daniela and Mueller, M. Gail and Natale, Lenina and Zucchetti, Ivana and De Santis, Rosaria and Pinto, Maria Rosaria and Litman, Gary W. and Gilbert, Jack A.},
abstractNote = {It is now widely understood that all animals engage in complex interactions with bacteria (or microbes) throughout their various life stages. This ancient exchange can involve cooperation and has resulted in a wide range of evolved host-microbial interdependencies, including those observed in the gut. Ciona intestinalis, a filter-feeding basal chordate and classic developmental model that can be experimentally manipulated, is being employed to help define these relationships. Ciona larvae are first exposed internally to microbes upon the initiation of feeding in metamorphosed individuals; however, whether or not these microbes subsequently colonize the gut and whether or not Ciona forms relationships with specific bacteria in the gut remains unknown. Here in this report, we show that the Ciona gut not only is colonized by a complex community of bacteria, but also that samples from three geographically isolated populations reveal striking similarity in abundant operational taxonomic units (OTUs) consistent with the selection of a core community by the gut ecosystem.},
doi = {10.1371/journal.pone.0093386},
journal = {PLoS ONE},
number = 4,
volume = 9,
place = {United States},
year = {2014},
month = {4}
}