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Title: Structural enzymology using X-ray free electron lasers

Authors:
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Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1395301
Alternate Identifier(s):
OSTI ID: 1369303
Grant/Contract Number:  
AC02-76SF00515; AC52-07NA27344
Resource Type:
Accepted Manuscript
Journal Name:
Structural Dynamics
Additional Journal Information:
Journal Volume: 4; Journal Issue: 4; Journal ID: ISSN 2329-7778
Publisher:
American Crystallographic Association/AIP
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Crystal structure; Electron densities of states; Enzymes; Enzyme kinetics; Biochemical reactions

Citation Formats

Kupitz, Christopher, Olmos, Jose L., Holl, Mark, Tremblay, Lee, Pande, Kanupriya, Pandey, Suraj, Oberthür, Dominik, Hunter, Mark, Liang, Mengning, Aquila, Andrew, Tenboer, Jason, Calvey, George, Katz, Andrea, Chen, Yujie, Wiedorn, Max O., Knoska, Juraj, Meents, Alke, Majriani, Valerio, Norwood, Tyler, Poudyal, Ishwor, Grant, Thomas, Miller, Mitchell D., Xu, Weijun, Tolstikova, Aleksandra, Morgan, Andrew, Metz, Markus, Martin-Garcia, Jose M., Zook, James D., Roy-Chowdhury, Shatabdi, Coe, Jesse, Nagaratnam, Nirupa, Meza, Domingo, Fromme, Raimund, Basu, Shibom, Frank, Matthias, White, Thomas, Barty, Anton, Bajt, Sasa, Yefanov, Oleksandr, Chapman, Henry N., Zatsepin, Nadia, Nelson, Garrett, Weierstall, Uwe, Spence, John, Schwander, Peter, Pollack, Lois, Fromme, Petra, Ourmazd, Abbas, Phillips, George N., and Schmidt, Marius. Structural enzymology using X-ray free electron lasers. United States: N. p., 2017. Web. doi:10.1063/1.4972069.
Kupitz, Christopher, Olmos, Jose L., Holl, Mark, Tremblay, Lee, Pande, Kanupriya, Pandey, Suraj, Oberthür, Dominik, Hunter, Mark, Liang, Mengning, Aquila, Andrew, Tenboer, Jason, Calvey, George, Katz, Andrea, Chen, Yujie, Wiedorn, Max O., Knoska, Juraj, Meents, Alke, Majriani, Valerio, Norwood, Tyler, Poudyal, Ishwor, Grant, Thomas, Miller, Mitchell D., Xu, Weijun, Tolstikova, Aleksandra, Morgan, Andrew, Metz, Markus, Martin-Garcia, Jose M., Zook, James D., Roy-Chowdhury, Shatabdi, Coe, Jesse, Nagaratnam, Nirupa, Meza, Domingo, Fromme, Raimund, Basu, Shibom, Frank, Matthias, White, Thomas, Barty, Anton, Bajt, Sasa, Yefanov, Oleksandr, Chapman, Henry N., Zatsepin, Nadia, Nelson, Garrett, Weierstall, Uwe, Spence, John, Schwander, Peter, Pollack, Lois, Fromme, Petra, Ourmazd, Abbas, Phillips, George N., & Schmidt, Marius. Structural enzymology using X-ray free electron lasers. United States. doi:10.1063/1.4972069.
Kupitz, Christopher, Olmos, Jose L., Holl, Mark, Tremblay, Lee, Pande, Kanupriya, Pandey, Suraj, Oberthür, Dominik, Hunter, Mark, Liang, Mengning, Aquila, Andrew, Tenboer, Jason, Calvey, George, Katz, Andrea, Chen, Yujie, Wiedorn, Max O., Knoska, Juraj, Meents, Alke, Majriani, Valerio, Norwood, Tyler, Poudyal, Ishwor, Grant, Thomas, Miller, Mitchell D., Xu, Weijun, Tolstikova, Aleksandra, Morgan, Andrew, Metz, Markus, Martin-Garcia, Jose M., Zook, James D., Roy-Chowdhury, Shatabdi, Coe, Jesse, Nagaratnam, Nirupa, Meza, Domingo, Fromme, Raimund, Basu, Shibom, Frank, Matthias, White, Thomas, Barty, Anton, Bajt, Sasa, Yefanov, Oleksandr, Chapman, Henry N., Zatsepin, Nadia, Nelson, Garrett, Weierstall, Uwe, Spence, John, Schwander, Peter, Pollack, Lois, Fromme, Petra, Ourmazd, Abbas, Phillips, George N., and Schmidt, Marius. Sat . "Structural enzymology using X-ray free electron lasers". United States. doi:10.1063/1.4972069. https://www.osti.gov/servlets/purl/1395301.
@article{osti_1395301,
title = {Structural enzymology using X-ray free electron lasers},
author = {Kupitz, Christopher and Olmos, Jose L. and Holl, Mark and Tremblay, Lee and Pande, Kanupriya and Pandey, Suraj and Oberthür, Dominik and Hunter, Mark and Liang, Mengning and Aquila, Andrew and Tenboer, Jason and Calvey, George and Katz, Andrea and Chen, Yujie and Wiedorn, Max O. and Knoska, Juraj and Meents, Alke and Majriani, Valerio and Norwood, Tyler and Poudyal, Ishwor and Grant, Thomas and Miller, Mitchell D. and Xu, Weijun and Tolstikova, Aleksandra and Morgan, Andrew and Metz, Markus and Martin-Garcia, Jose M. and Zook, James D. and Roy-Chowdhury, Shatabdi and Coe, Jesse and Nagaratnam, Nirupa and Meza, Domingo and Fromme, Raimund and Basu, Shibom and Frank, Matthias and White, Thomas and Barty, Anton and Bajt, Sasa and Yefanov, Oleksandr and Chapman, Henry N. and Zatsepin, Nadia and Nelson, Garrett and Weierstall, Uwe and Spence, John and Schwander, Peter and Pollack, Lois and Fromme, Petra and Ourmazd, Abbas and Phillips, George N. and Schmidt, Marius},
abstractNote = {},
doi = {10.1063/1.4972069},
journal = {Structural Dynamics},
number = 4,
volume = 4,
place = {United States},
year = {2017},
month = {7}
}

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Figures / Tables:

FIG. 1 FIG. 1: Data collection schematic showing the T-junction set-up used for a mixing time of about 2 s. The T-junction was placed outside of the nozzle rod in our experiment but could also be engineered to fit inside closer to the interaction region for shorter mixing times.

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        Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.