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Title: Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy

The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM). Different sample preparation approaches were used to match each imaging technique. While preparation for SIM included cell fixation and staining, intact frozen hydrated cells were used for the trace element imaging by hard X-ray fluorescence and exploration of the structural features by soft X-ray absorption tomography. In conclusion, each technique documented differences between samples with regard to morphology and elemental composition and underscored the importance of use of multiple patient derived samples for detailed GBM study.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5] ;  [3] ;  [2]
  1. CELLS - ALBA, Cerdanyola del Valles (Spain)
  2. Northwestern Univ., Chicago, IL (United States)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
  4. Georg-August Univ. Medical Centre, Gottingen (Germany)
  5. Northwestern Univ., Evanston, IL (United States)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
Analyst
Additional Journal Information:
Journal Volume: 142; Journal Issue: 2; Journal ID: ISSN 0003-2654
Publisher:
Royal Society of Chemistry
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1395177

Ducic, Tanja, Paunesku, Tatjana, Chen, Si, Ninkovic, Milena, Speling, Swetlana, Wilke, Charlene, Lai, Barry, and Woloschak, Gayle. Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy. United States: N. p., Web. doi:10.1039/C6AN02532C.
Ducic, Tanja, Paunesku, Tatjana, Chen, Si, Ninkovic, Milena, Speling, Swetlana, Wilke, Charlene, Lai, Barry, & Woloschak, Gayle. Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy. United States. doi:10.1039/C6AN02532C.
Ducic, Tanja, Paunesku, Tatjana, Chen, Si, Ninkovic, Milena, Speling, Swetlana, Wilke, Charlene, Lai, Barry, and Woloschak, Gayle. 2016. "Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy". United States. doi:10.1039/C6AN02532C. https://www.osti.gov/servlets/purl/1395177.
@article{osti_1395177,
title = {Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy},
author = {Ducic, Tanja and Paunesku, Tatjana and Chen, Si and Ninkovic, Milena and Speling, Swetlana and Wilke, Charlene and Lai, Barry and Woloschak, Gayle},
abstractNote = {The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM). Different sample preparation approaches were used to match each imaging technique. While preparation for SIM included cell fixation and staining, intact frozen hydrated cells were used for the trace element imaging by hard X-ray fluorescence and exploration of the structural features by soft X-ray absorption tomography. In conclusion, each technique documented differences between samples with regard to morphology and elemental composition and underscored the importance of use of multiple patient derived samples for detailed GBM study.},
doi = {10.1039/C6AN02532C},
journal = {Analyst},
number = 2,
volume = 142,
place = {United States},
year = {2016},
month = {12}
}