Katanin spiral and ring structures shed light on power stroke for microtubule severing
Abstract
Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of 3D structures, their mechanism has remained poorly understood. We report the first X-ray structure of the monomeric AAA katanin module and cryo-EM reconstructions of the hexamer in two conformations. These reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to severing enzymes and critical for their function. Our reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes inter-protomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.
- Authors:
-
[4]
- National Inst. of Neurological Disorders and Stroke, Bethesda, MD (United States). Cell Biology and Biophysics Unit, Porter Neuroscience Research Center
- Biophysics Core, National Heart, Lung and Blood Inst., Bethesda, MD (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- National Inst. of Neurological Disorders and Stroke, Bethesda, MD (United States). Cell Biology and Biophysics Unit, Porter Neuroscience Research Center; Biophysics Core, National Heart, Lung and Blood Inst., Bethesda, MD (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1394618
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 24; Journal Issue: 9; Journal ID: ISSN 1545-9993
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; protein SAXS
Citation Formats
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, and Roll-Mecak, Antonina. Katanin spiral and ring structures shed light on power stroke for microtubule severing. United States: N. p., 2017.
Web. doi:10.1038/nsmb.3448.
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, & Roll-Mecak, Antonina. Katanin spiral and ring structures shed light on power stroke for microtubule severing. United States. doi:10.1038/nsmb.3448.
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, and Roll-Mecak, Antonina. Mon .
"Katanin spiral and ring structures shed light on power stroke for microtubule severing". United States. doi:10.1038/nsmb.3448. https://www.osti.gov/servlets/purl/1394618.
@article{osti_1394618,
title = {Katanin spiral and ring structures shed light on power stroke for microtubule severing},
author = {Zehr, Elena and Szyk, Agnieszka and Piszczek, Grzegorz and Szczesna, Ewa and Zuo, Xiaobing and Roll-Mecak, Antonina},
abstractNote = {Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of 3D structures, their mechanism has remained poorly understood. We report the first X-ray structure of the monomeric AAA katanin module and cryo-EM reconstructions of the hexamer in two conformations. These reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to severing enzymes and critical for their function. Our reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes inter-protomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.},
doi = {10.1038/nsmb.3448},
journal = {Nature Structural & Molecular Biology},
number = 9,
volume = 24,
place = {United States},
year = {2017},
month = {8}
}
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