Katanin spiral and ring structures shed light on power stroke for microtubule severing
Abstract
Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of 3D structures, their mechanism has remained poorly understood. We report the first X-ray structure of the monomeric AAA katanin module and cryo-EM reconstructions of the hexamer in two conformations. These reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to severing enzymes and critical for their function. Our reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes inter-protomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.
- Authors:
-
- National Inst. of Neurological Disorders and Stroke, Bethesda, MD (United States). Cell Biology and Biophysics Unit, Porter Neuroscience Research Center
- Biophysics Core, National Heart, Lung and Blood Inst., Bethesda, MD (United States)
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- National Inst. of Neurological Disorders and Stroke, Bethesda, MD (United States). Cell Biology and Biophysics Unit, Porter Neuroscience Research Center; Biophysics Core, National Heart, Lung and Blood Inst., Bethesda, MD (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1394618
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 24; Journal Issue: 9; Journal ID: ISSN 1545-9993
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; protein SAXS
Citation Formats
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, and Roll-Mecak, Antonina. Katanin spiral and ring structures shed light on power stroke for microtubule severing. United States: N. p., 2017.
Web. doi:10.1038/nsmb.3448.
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, & Roll-Mecak, Antonina. Katanin spiral and ring structures shed light on power stroke for microtubule severing. United States. doi:10.1038/nsmb.3448.
Zehr, Elena, Szyk, Agnieszka, Piszczek, Grzegorz, Szczesna, Ewa, Zuo, Xiaobing, and Roll-Mecak, Antonina. Mon .
"Katanin spiral and ring structures shed light on power stroke for microtubule severing". United States. doi:10.1038/nsmb.3448. https://www.osti.gov/servlets/purl/1394618.
@article{osti_1394618,
title = {Katanin spiral and ring structures shed light on power stroke for microtubule severing},
author = {Zehr, Elena and Szyk, Agnieszka and Piszczek, Grzegorz and Szczesna, Ewa and Zuo, Xiaobing and Roll-Mecak, Antonina},
abstractNote = {Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of 3D structures, their mechanism has remained poorly understood. We report the first X-ray structure of the monomeric AAA katanin module and cryo-EM reconstructions of the hexamer in two conformations. These reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to severing enzymes and critical for their function. Our reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes inter-protomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.},
doi = {10.1038/nsmb.3448},
journal = {Nature Structural & Molecular Biology},
number = 9,
volume = 24,
place = {United States},
year = {2017},
month = {8}
}
Web of Science
Works referenced in this record:
A Mechanism for Reorientation of Cortical Microtubule Arrays Driven by Microtubule Severing
journal, November 2013
- Lindeboom, J. J.; Nakamura, M.; Hibbel, A.
- Science, Vol. 342, Issue 6163
The Cul3/Klhdc5 E3 Ligase Regulates p60/Katanin and Is Required for Normal Mitosis in Mammalian Cells
journal, March 2009
- Cummings, Cristina M.; Bentley, Cornelia A.; Perdue, Sarah A.
- Journal of Biological Chemistry, Vol. 284, Issue 17
Uniqueness of ab initio shape determination in small-angle scattering
journal, April 2003
- Volkov, Vladimir V.; Svergun, Dmitri I.
- Journal of Applied Crystallography, Vol. 36, Issue 3, p. 860-864
Vps4 disassembles an ESCRT-III filament by global unfolding and processive translocation
journal, May 2015
- Yang, Bei; Stjepanovic, Goran; Shen, Qingtao
- Nature Structural & Molecular Biology, Vol. 22, Issue 6
Complete subunit architecture of the proteasome regulatory particle
journal, January 2012
- Lander, Gabriel C.; Estrin, Eric; Matyskiela, Mary E.
- Nature, Vol. 482, Issue 7384
Mutations in KATNB1 Cause Complex Cerebral Malformations by Disrupting Asymmetrically Dividing Neural Progenitors
journal, December 2014
- Mishra-Gorur, Ketu; Çağlayan, Ahmet Okay; Schaffer, Ashleigh E.
- Neuron, Vol. 84, Issue 6
Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6
journal, May 2015
- Grant, Timothy; Grigorieff, Nikolaus
- eLife, Vol. 4
Mechanochemical basis of protein degradation by a double-ring AAA+ machine
journal, September 2014
- Olivares, Adrian O.; Nager, Andrew R.; Iosefson, Ohad
- Nature Structural & Molecular Biology, Vol. 21, Issue 10
Katanin p80 Regulates Human Cortical Development by Limiting Centriole and Cilia Number
journal, December 2014
- Hu, Wen F.; Pomp, Oz; Ben-Omran, Tawfeg
- Neuron, Vol. 84, Issue 6
A syndrome of microcephaly, short stature, polysyndactyly, and dental anomalies caused by a homozygous KATNB1 mutation
journal, December 2015
- Yigit, Gökhan; Wieczorek, Dagmar; Bögershausen, Nina
- American Journal of Medical Genetics Part A, Vol. 170, Issue 3
Bsoft: Image and Molecular Processing in Electron Microscopy
journal, February 2001
- Heymann, J. Bernard
- Journal of Structural Biology, Vol. 133, Issue 2-3
Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor
journal, November 2016
- Thomsen, Nathan D.; Lawson, Michael R.; Witkowsky, Lea B.
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 48
Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
journal, February 2007
- Mylonas, Efstratios; Svergun, Dmitri I.
- Journal of Applied Crystallography, Vol. 40, Issue s1
Determination of the regularization parameter in indirect-transform methods using perceptual criteria
journal, August 1992
- Svergun, D. I.
- Journal of Applied Crystallography, Vol. 25, Issue 4, p. 495-503
Identification of katanin, an ATPase that severs and disassembles stable microtubules
journal, November 1993
- McNally, Francis J.; Vale, Ronald D.
- Cell, Vol. 75, Issue 3
Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin
journal, January 2008
- Roll-Mecak, Antonina; Vale, Ronald D.
- Nature, Vol. 451, Issue 7176
Microtubule-severing enzymes
journal, February 2010
- Roll-Mecak, Antonina; McNally, Francis J.
- Current Opinion in Cell Biology, Vol. 22, Issue 1
Structural and mechanistic studies of VPS4 proteins
journal, September 2005
- Scott, Anna; Chung, Hyo-Young; Gonciarz-Swiatek, Malgorzata
- The EMBO Journal, Vol. 24, Issue 20
Crystal structure of the human spastin AAA domain
journal, August 2012
- Taylor, Jennifer L.; White, Susan Roehl; Lauring, Brett
- Journal of Structural Biology, Vol. 179, Issue 2
Running in Reverse: The Structural Basis for Translocation Polarity in Hexameric Helicases
journal, October 2009
- Thomsen, Nathan D.; Berger, James M.
- Cell, Vol. 139, Issue 3
CTFFIND4: Fast and accurate defocus estimation from electron micrographs
journal, November 2015
- Rohou, Alexis; Grigorieff, Nikolaus
- Journal of Structural Biology, Vol. 192, Issue 2
Severing of stable microtubules by a mitotically activated protein in xenopus egg extracts
journal, February 1991
- Vale, Ronald D.
- Cell, Vol. 64, Issue 4
MolProbity: all-atom contacts and structure validation for proteins and nucleic acids
journal, May 2007
- Davis, I. W.; Leaver-Fay, A.; Chen, V. B.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Mechanism of DNA translocation in a replicative hexameric helicase
journal, July 2006
- Enemark, Eric J.; Joshua-Tor, Leemor
- Nature, Vol. 442, Issue 7100
Katanin Disrupts the Microtubule Lattice and Increases Polymer Number in C. elegans Meiosis
journal, October 2006
- Srayko, Martin; O'Toole, Eileen T.; Hyman, Anthony A.
- Current Biology, Vol. 16, Issue 19
Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro
journal, April 2014
- McNally, Karen; Berg, Evan; Cortes, Daniel B.
- Molecular Biology of the Cell, Vol. 25, Issue 7
The Drosophila Homologue of the Hereditary Spastic Paraplegia Protein, Spastin, Severs and Disassembles Microtubules
journal, April 2005
- Roll-Mecak, Antonina; Vale, Ronald D.
- Current Biology, Vol. 15, Issue 7
The Microtubule-severing Proteins Spastin and Katanin Participate Differently in the Formation of Axonal Branches
journal, April 2008
- Yu, Wenqian; Qiang, Liang; Solowska, Joanna M.
- Molecular Biology of the Cell, Vol. 19, Issue 4
Size-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling
journal, March 2000
- Schuck, Peter
- Biophysical Journal, Vol. 78, Issue 3
Mechanistic insights into the recycling machine of the SNARE complex
journal, January 2015
- Zhao, Minglei; Wu, Shenping; Zhou, Qiangjun
- Nature, Vol. 518, Issue 7537
N-Terminal Phosphorylation of p60 Katanin Directly Regulates Microtubule Severing
journal, January 2013
- Whitehead, Evan; Heald, Rebecca; Wilbur, Jeremy D.
- Journal of Molecular Biology, Vol. 425, Issue 2
Katanin regulates dynamics of microtubules and biogenesis of motile cilia
journal, September 2007
- Sharma, Neeraj; Bryant, Jessica; Wloga, Dorota
- The Journal of Cell Biology, Vol. 178, Issue 6
Microtubule Disassembly by ATP-Dependent Oligomerization of the AAA Enzyme Katanin
journal, October 1999
- Hartman, J. J.
- Science, Vol. 286, Issue 5440
Microtubule Severing at Crossover Sites by Katanin Generates Ordered Cortical Microtubule Arrays in Arabidopsis
journal, November 2013
- Zhang, Quan; Fishel, Erica; Bertroche, Tyler
- Current Biology, Vol. 23, Issue 21
Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape
journal, February 1993
- Gittes, F.
- The Journal of Cell Biology, Vol. 120, Issue 4
Structure of a AAA+ unfoldase in the process of unfolding substrate
journal, April 2017
- Ripstein, Zev A.; Huang, Rui; Augustyniak, Rafal
- eLife, Vol. 6
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Flexible Fitting of Atomic Structures into Electron Microscopy Maps Using Molecular Dynamics
journal, May 2008
- Trabuco, Leonardo G.; Villa, Elizabeth; Mitra, Kakoli
- Structure, Vol. 16, Issue 5
3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM
journal, August 2016
- Chua, Eugene Y. D.; Vogirala, Vinod K.; Inian, Oviya
- Nucleic Acids Research, Vol. 44, Issue 17
Structures of Asymmetric ClpX Hexamers Reveal Nucleotide-Dependent Motions in a AAA+ Protein-Unfolding Machine
journal, November 2009
- Glynn, Steven E.; Martin, Andreas; Nager, Andrew R.
- Cell, Vol. 139, Issue 4
Operational Plasticity Enables Hsp104 to Disaggregate Diverse Amyloid and Nonamyloid Clients
journal, November 2012
- DeSantis, Morgan E.; Leung, Eunice H.; Sweeny, Elizabeth A.
- Cell, Vol. 151, Issue 4
FREALIGN: High-resolution refinement of single particle structures
journal, January 2007
- Grigorieff, Nikolaus
- Journal of Structural Biology, Vol. 157, Issue 1
A Common Substrate Recognition Mode Conserved between Katanin p60 and VPS4 Governs Microtubule Severing and Membrane Skeleton Reorganization
journal, March 2010
- Iwaya, Naoko; Kuwahara, Yohta; Fujiwara, Yoshie
- Journal of Biological Chemistry, Vol. 285, Issue 22
Structural insights into the functional cycle of the ATPase module of the 26S proteasome
journal, January 2017
- Wehmer, Marc; Rudack, Till; Beck, Florian
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 6
Mechanism of Vps4 hexamer function revealed by cryo-EM
journal, April 2017
- Su, Min; Guo, Emily Z.; Ding, Xinqiang
- Science Advances, Vol. 3, Issue 4
Spiral architecture of the Hsp104 disaggregase reveals the basis for polypeptide translocation
journal, August 2016
- Yokom, Adam L.; Gates, Stephanie N.; Jackrel, Meredith E.
- Nature Structural & Molecular Biology, Vol. 23, Issue 9
Spectrum of SPG4 mutations in autosomal dominant spastic paraplegia
journal, March 2000
- Fonknechten, N.
- Human Molecular Genetics, Vol. 9, Issue 4
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro
journal, April 2014
- McNally, Karen; Berg, Evan; Cortes, Daniel B.
- Molecular Biology of the Cell, Vol. 25, Issue 7
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012
- Scheres, Sjors H. W.
- Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530
Microtubule-severing activity of the AAA+ ATPase Katanin is essential for female meiotic spindle assembly
journal, August 2016
- Joly, Nicolas; Martino, Lisa; Gigant, Emmanuelle
- Development, Vol. 143, Issue 19
2.3 A resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition
journal, January 2016
- Banerjee, S.; Bartesaghi, A.; Merk, A.
- Science, Vol. 351, Issue 6275
Restoring Low Resolution Structure of Biological Macromolecules from Solution Scattering Using Simulated Annealing
journal, June 1999
- Svergun, D. I.
- Biophysical Journal, Vol. 76, Issue 6, p. 2879-2886
An Essential Role for Katanin in Severing Microtubules in the Neuron
journal, April 1999
- Ahmad, Fridoon J.; Yu, Wenqian; McNally, Francis J.
- The Journal of Cell Biology, Vol. 145, Issue 2
Katanin Severing and Binding Microtubules Are Inhibited by Tubulin Carboxy Tails
journal, December 2015
- Bailey, Megan E.; Sackett, Dan L.; Ross, Jennifer L.
- Biophysical Journal, Vol. 109, Issue 12
An Essential Role for Katanin in Severing Microtubules in the Neuron
journal, April 1999
- Ahmad, Fridoon J.; Yu, Wenqian; McNally, Francis J.
- The Journal of Cell Biology, Vol. 145, Issue 2
Microtubule Severing by Katanin p60 AAA+ ATPase Requires the C-terminal Acidic Tails of Both α- and β-Tubulins and Basic Amino Acid Residues in the AAA+ Ring Pore
journal, March 2015
- Johjima, Ai; Noi, Kentaro; Nishikori, Shingo
- Journal of Biological Chemistry, Vol. 290, Issue 18
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Katanin Contributes to Interspecies Spindle Length Scaling in Xenopus
journal, December 2011
- Loughlin, Rose; Wilbur, Jeremy D.; McNally, Francis J.
- Cell, Vol. 147, Issue 6
AAA+ proteins: have engine, will work
journal, July 2005
- Hanson, Phyllis I.; Whiteheart, Sidney W.
- Nature Reviews Molecular Cell Biology, Vol. 6, Issue 7
Crystal Structure of the Hexamerization Domain of N-ethylmaleimide–Sensitive Fusion Protein
journal, August 1998
- Lenzen, Christian U.; Steinmann, Diana; Whiteheart, Sidney W.
- Cell, Vol. 94, Issue 4
DnaK Chaperone-dependent Disaggregation by Caseinolytic Peptidase B (ClpB) Mutants Reveals Functional Overlap in the N-terminal Domain and Nucleotide-binding Domain-1 Pore Tyrosine
journal, June 2012
- Doyle, Shannon M.; Hoskins, Joel R.; Wickner, Sue
- Journal of Biological Chemistry, Vol. 287, Issue 34
Katanin, a Microtubule-Severing Protein, Is a Novel AAA ATPase that Targets to the Centrosome Using a WD40-Containing Subunit
journal, April 1998
- Hartman, James J.; Mahr, Jeff; McNally, Karen
- Cell, Vol. 93, Issue 2
The Microtubule-severing Proteins Spastin and Katanin Participate Differently in the Formation of Axonal Branches
journal, April 2008
- Yu, Wenqian; Qiang, Liang; Solowska, Joanna M.
- Molecular Biology of the Cell, Vol. 19, Issue 4
Three microtubule severing enzymes contribute to the “Pacman-flux” machinery that moves chromosomes
journal, April 2007
- Zhang, Dong; Rogers, Gregory C.; Buster, Daniel W.
- The Journal of Cell Biology, Vol. 177, Issue 2
Recognition of C-terminal amino acids in tubulin by pore loops in Spastin is important for microtubule severing
journal, March 2007
- White, Susan Roehl; Evans, Katia J.; Lary, Jeffrey
- The Journal of Cell Biology, Vol. 176, Issue 7
Graded Control of Microtubule Severing by Tubulin Glutamylation
journal, February 2016
- Valenstein, Max L.; Roll-Mecak, Antonina
- Cell, Vol. 164, Issue 5
Situs: A Package for Docking Crystal Structures into Low-Resolution Maps from Electron Microscopy
journal, April 1999
- Wriggers, Willy; Milligan, Ronald A.; McCammon, J. Andrew
- Journal of Structural Biology, Vol. 125, Issue 2-3
Automated electron microscope tomography using robust prediction of specimen movements
journal, October 2005
- Mastronarde, David N.
- Journal of Structural Biology, Vol. 152, Issue 1
Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase
journal, April 2017
- Monroe, Nicole; Han, Han; Shen, Peter S.
- eLife, Vol. 6
Accurate assessment of mass, models and resolution by small-angle scattering
journal, April 2013
- Rambo, Robert P.; Tainer, John A.
- Nature, Vol. 496, Issue 7446
[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997
- Otwinowski, Zbyszek; Minor, Wladek
- Macromolecular Crystallography Part A, 307-326
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Katanin controls mitotic and meiotic spindle length
journal, December 2006
- McNally, Karen; Audhya, Anjon; Oegema, Karen
- The Journal of Cell Biology, Vol. 175, Issue 6
Structure and function of the AAA+ nucleotide binding pocket
journal, January 2012
- Wendler, Petra; Ciniawsky, Susanne; Kock, Malte
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol. 1823, Issue 1
EMAN: Semiautomated Software for High-Resolution Single-Particle Reconstructions
journal, December 1999
- Ludtke, Steven J.; Baldwin, Philip R.; Chiu, Wah
- Journal of Structural Biology, Vol. 128, Issue 1
Asymmetric ring structure of Vps4 required for ESCRT-III disassembly
journal, December 2015
- Caillat, Christophe; Macheboeuf, Pauline; Wu, Yuanfei
- Nature Communications, Vol. 6, Issue 1
Works referencing / citing this record:
Microtubule-severing enzymes: From cellular functions to molecular mechanism
journal, October 2018
- McNally, Francis J.; Roll-Mecak, Antonina
- The Journal of Cell Biology, Vol. 217, Issue 12
Microtubule-severing enzymes: From cellular functions to molecular mechanism
journal, October 2018
- McNally, Francis J.; Roll-Mecak, Antonina
- The Journal of Cell Biology, Vol. 217, Issue 12
The AAA protein spastin possesses two levels of basal ATP ase activity
journal, May 2018
- Fan, Xiangyu; Lin, Zhijie; Fan, Guanghui
- FEBS Letters, Vol. 592, Issue 10
Katanin catalyzes microtubule depolymerization independently of tubulin C‐terminal tails
journal, March 2019
- Belonogov, Liudmila; Bailey, Megan E.; Tyler, Madison A.
- Cytoskeleton, Vol. 76, Issue 3
Stairway to translocation: AAA+ motor structures reveal the mechanisms of ATP‐dependent substrate translocation
journal, October 2019
- Gates, Stephanie N.; Martin, Andreas
- Protein Science, Vol. 29, Issue 2
Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
journal, January 2019
- Lo, Yu-Hua; Sobhany, Mack; Hsu, Allen L.
- Nature Communications, Vol. 10, Issue 1
Autonomous helical propagation of active toroids with mechanical action
journal, March 2019
- Shen, Bowen; Zhu, Youliang; Kim, Yongju
- Nature Communications, Vol. 10, Issue 1
The molecular principles governing the activity and functional diversity of AAA+ proteins
journal, November 2019
- Puchades, Cristina; Sandate, Colby R.; Lander, Gabriel C.
- Nature Reviews Molecular Cell Biology, Vol. 21, Issue 1
An allosteric network in spastin couples multiple activities required for microtubule severing
journal, July 2019
- Sandate, Colby R.; Szyk, Agnieszka; Zehr, Elena A.
- Nature Structural & Molecular Biology, Vol. 26, Issue 8
Structural insights into ATP hydrolysis by the MoxR ATPase RavA and the LdcI-RavA cage-like complex
journal, January 2020
- Jessop, Matthew; Arragain, Benoit; Miras, Roger
- Communications Biology, Vol. 3, Issue 1
Structure and mechanism of the ESCRT pathway AAA+ ATPase Vps4
journal, January 2019
- Han, Han; Hill, Christopher P.
- Biochemical Society Transactions, Vol. 47, Issue 1
Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation
journal, November 2019
- Han, Han; Schubert, Heidi L.; McCullough, John
- Journal of Biological Chemistry, Vol. 295, Issue 2
Spiraling in Control: Structures and Mechanisms of the Hsp104 Disaggregase
journal, February 2019
- Shorter, James; Southworth, Daniel R.
- Cold Spring Harbor Perspectives in Biology, Vol. 11, Issue 8
Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing
journal, November 2017
- Puchades, Cristina; Rampello, Anthony J.; Shin, Mia
- Science, Vol. 358, Issue 6363
Severing enzymes amplify microtubule arrays through lattice GTP-tubulin incorporation
journal, August 2018
- Vemu, Annapurna; Szczesna, Ewa; Zehr, Elena A.
- Science, Vol. 361, Issue 6404
A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery
journal, October 2019
- Pedrosa, Ana G.; Francisco, Tânia; Ferreira, Maria J.
- International Journal of Molecular Sciences, Vol. 20, Issue 21
The AAA ATPase Vps4 binds ESCRT-III substrates through a repeating array of dipeptide-binding pockets
journal, November 2017
- Han, Han; Monroe, Nicole; Sundquist, Wesley I.
- eLife, Vol. 6