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Title: Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46

Abstract

Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.

Authors:
 [1];  [2];  [1]; ORCiD logo [1]
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biomedical and Diagnostic Sciences
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division. Mass Spectrometry and Laser Spectrometry
Publication Date:
Research Org.:
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; Tennessee Center of Excellence in Livestock Diseases and Human Health (United States)
OSTI Identifier:
1394588
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 8; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Staphylococcus aureus; sequence motif analysis; polymerase chain reaction; antibodies; flow cytometry; gene expression; protein expression; peptidoglycans

Citation Formats

Balachandran, Manasi, Giannone, Richard J., Bemis, David A., and Kania, Stephen A. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0183913.
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., & Kania, Stephen A. Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46. United States. doi:10.1371/journal.pone.0183913.
Balachandran, Manasi, Giannone, Richard J., Bemis, David A., and Kania, Stephen A. Thu . "Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46". United States. doi:10.1371/journal.pone.0183913. https://www.osti.gov/servlets/purl/1394588.
@article{osti_1394588,
title = {Molecular basis of surface anchored protein A deficiency in the Staphylococcus aureus strain Wood 46},
author = {Balachandran, Manasi and Giannone, Richard J. and Bemis, David A. and Kania, Stephen A.},
abstractNote = {Protein A in Staphylococcus aureus is encoded by the spa (staphylococcal protein A) gene and binds to immunoglobulin (Ig). The S. aureus strain Wood 46 has been variously reported as protein A-deficient and/or spa negative and used as a control in animal models of staphylococcal infections. The results of this study indicate that Wood 46 has normal spa expression but transcribes very low levels of the srtA gene which encodes the sortase A (SrtA) enzyme. This is consistent with unique mutations in the srtA promoter. In this study, a low level of sortase A explains deficient anchoring of proteins with an LPXTG motif, such as protein A, fibrinogen-binding protein and fibronectin-binding proteins A and B on to the peptidoglycan cell wall. The activity of secreted protein A is an important consideration for use of Wood 46 in functional experiments and animal models.},
doi = {10.1371/journal.pone.0183913},
journal = {PLoS ONE},
number = 8,
volume = 12,
place = {United States},
year = {2017},
month = {8}
}

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Works referenced in this record:

Anchoring of Surface Proteins to the Cell Wall of Staphylococcus aureus : SORTASE CATALYZED
journal, March 2000

  • Ton-That, Hung; Mazmanian, Sarkis K.; Faull, Kym F.
  • Journal of Biological Chemistry, Vol. 275, Issue 13
  • DOI: 10.1074/jbc.275.13.9876

Protein secretion and surface display in Gram-positive bacteria
journal, April 2012

  • Schneewind, Olaf; Missiakas, Dominique M.
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 367, Issue 1592
  • DOI: 10.1098/rstb.2011.0210

Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
journal, May 2001

  • Ilangovan, U.; Ton-That, H.; Iwahara, J.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 11
  • DOI: 10.1073/pnas.101064198

Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus
journal, June 2001


Progress Toward a Staphylococcus aureus Vaccine
journal, December 2011

  • Daum, R. S.; Spellberg, B.
  • Clinical Infectious Diseases, Vol. 54, Issue 4
  • DOI: 10.1093/cid/cir828

Staphylococcus aureus Infections
journal, August 1998


Release of protein A from the cell wall of Staphylococcus aureus
journal, January 2014

  • Becker, S.; Frankel, M. B.; Schneewind, O.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 4
  • DOI: 10.1073/pnas.1317181111

Recurrent infections and immune evasion strategies of Staphylococcus aureus
journal, February 2012

  • Kim, Hwan Keun; Thammavongsa, Vilasack; Schneewind, Olaf
  • Current Opinion in Microbiology, Vol. 15, Issue 1
  • DOI: 10.1016/j.mib.2011.10.012