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Title: A study on the cytotoxicity of carbon-based materials

Abstract

With an aim to understand the origin and key contributing factors towards carboninduced cytotoxicity, we have studied five different carbon samples with diverse surface area, pore width, shape and size, conductivity and surface functionality. All the carbon materials were characterized with surface area and pore size distribution, x-ray photoelectron spectroscopy (XPS) and electron microscopic imaging. We performed cytotoxicity study in Caco-2 cells by colorimetric assay, oxidative stress analysis by reactive oxygen species (ROX) detection, cellular metabolic activity measurement by adenosine triphosphate (ATP) depletion and visualization of cellular internalization by TEM imaging. The carbon materials demonstrated a varying degree of cytotoxicity in contact with Caco-2 cells. The lowest cell survival rate was observed for nanographene, which possessed the minimal size amongst all the carbon samples under study. None of the carbons induced oxidative stress to the cells as indicated by the ROX generation results. Cellular metabolic activity study revealed that the carbon materials caused ATP depletion in cells and nanographene caused the highest depletion. Visual observation by TEM imaging indicated the cellular internalization of nanographene. This study confirmed that the size is the key cause of carbon-induced cytotoxicity and it is probably caused by the ATP depletion within the cell.

Authors:
 [1];  [2];  [2];  [2];  [3];  [2]
  1. Widener Univ., Chester, PA (United States)
  2. Michigan Technological Univ., Houghton, MI (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1394434
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems
Additional Journal Information:
Journal Volume: 68; Journal Issue: C; Journal ID: ISSN 0928-4931
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, and Saksule, Ashish. A study on the cytotoxicity of carbon-based materials. United States: N. p., 2016. Web. doi:10.1016/j.msec.2016.05.094.
Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, & Saksule, Ashish. A study on the cytotoxicity of carbon-based materials. United States. doi:10.1016/j.msec.2016.05.094.
Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, and Saksule, Ashish. Wed . "A study on the cytotoxicity of carbon-based materials". United States. doi:10.1016/j.msec.2016.05.094. https://www.osti.gov/servlets/purl/1394434.
@article{osti_1394434,
title = {A study on the cytotoxicity of carbon-based materials},
author = {Saha, Dipendu and Heldt, Caryn L. and Gencoglu, Maria F. and Vijayaragavan, K. Saagar and Chen, Jihua and Saksule, Ashish},
abstractNote = {With an aim to understand the origin and key contributing factors towards carboninduced cytotoxicity, we have studied five different carbon samples with diverse surface area, pore width, shape and size, conductivity and surface functionality. All the carbon materials were characterized with surface area and pore size distribution, x-ray photoelectron spectroscopy (XPS) and electron microscopic imaging. We performed cytotoxicity study in Caco-2 cells by colorimetric assay, oxidative stress analysis by reactive oxygen species (ROX) detection, cellular metabolic activity measurement by adenosine triphosphate (ATP) depletion and visualization of cellular internalization by TEM imaging. The carbon materials demonstrated a varying degree of cytotoxicity in contact with Caco-2 cells. The lowest cell survival rate was observed for nanographene, which possessed the minimal size amongst all the carbon samples under study. None of the carbons induced oxidative stress to the cells as indicated by the ROX generation results. Cellular metabolic activity study revealed that the carbon materials caused ATP depletion in cells and nanographene caused the highest depletion. Visual observation by TEM imaging indicated the cellular internalization of nanographene. This study confirmed that the size is the key cause of carbon-induced cytotoxicity and it is probably caused by the ATP depletion within the cell.},
doi = {10.1016/j.msec.2016.05.094},
journal = {Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems},
number = C,
volume = 68,
place = {United States},
year = {2016},
month = {5}
}

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