A study on the cytotoxicity of carbon-based materials
Abstract
With an aim to understand the origin and key contributing factors towards carboninduced cytotoxicity, we have studied five different carbon samples with diverse surface area, pore width, shape and size, conductivity and surface functionality. All the carbon materials were characterized with surface area and pore size distribution, x-ray photoelectron spectroscopy (XPS) and electron microscopic imaging. We performed cytotoxicity study in Caco-2 cells by colorimetric assay, oxidative stress analysis by reactive oxygen species (ROX) detection, cellular metabolic activity measurement by adenosine triphosphate (ATP) depletion and visualization of cellular internalization by TEM imaging. The carbon materials demonstrated a varying degree of cytotoxicity in contact with Caco-2 cells. The lowest cell survival rate was observed for nanographene, which possessed the minimal size amongst all the carbon samples under study. None of the carbons induced oxidative stress to the cells as indicated by the ROX generation results. Cellular metabolic activity study revealed that the carbon materials caused ATP depletion in cells and nanographene caused the highest depletion. Visual observation by TEM imaging indicated the cellular internalization of nanographene. This study confirmed that the size is the key cause of carbon-induced cytotoxicity and it is probably caused by the ATP depletion within the cell.
- Authors:
-
- Widener Univ., Chester, PA (United States)
- Michigan Technological Univ., Houghton, MI (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1394434
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems
- Additional Journal Information:
- Journal Volume: 68; Journal Issue: C; Journal ID: ISSN 0928-4931
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, and Saksule, Ashish. A study on the cytotoxicity of carbon-based materials. United States: N. p., 2016.
Web. doi:10.1016/j.msec.2016.05.094.
Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, & Saksule, Ashish. A study on the cytotoxicity of carbon-based materials. United States. https://doi.org/10.1016/j.msec.2016.05.094
Saha, Dipendu, Heldt, Caryn L., Gencoglu, Maria F., Vijayaragavan, K. Saagar, Chen, Jihua, and Saksule, Ashish. Wed .
"A study on the cytotoxicity of carbon-based materials". United States. https://doi.org/10.1016/j.msec.2016.05.094. https://www.osti.gov/servlets/purl/1394434.
@article{osti_1394434,
title = {A study on the cytotoxicity of carbon-based materials},
author = {Saha, Dipendu and Heldt, Caryn L. and Gencoglu, Maria F. and Vijayaragavan, K. Saagar and Chen, Jihua and Saksule, Ashish},
abstractNote = {With an aim to understand the origin and key contributing factors towards carboninduced cytotoxicity, we have studied five different carbon samples with diverse surface area, pore width, shape and size, conductivity and surface functionality. All the carbon materials were characterized with surface area and pore size distribution, x-ray photoelectron spectroscopy (XPS) and electron microscopic imaging. We performed cytotoxicity study in Caco-2 cells by colorimetric assay, oxidative stress analysis by reactive oxygen species (ROX) detection, cellular metabolic activity measurement by adenosine triphosphate (ATP) depletion and visualization of cellular internalization by TEM imaging. The carbon materials demonstrated a varying degree of cytotoxicity in contact with Caco-2 cells. The lowest cell survival rate was observed for nanographene, which possessed the minimal size amongst all the carbon samples under study. None of the carbons induced oxidative stress to the cells as indicated by the ROX generation results. Cellular metabolic activity study revealed that the carbon materials caused ATP depletion in cells and nanographene caused the highest depletion. Visual observation by TEM imaging indicated the cellular internalization of nanographene. This study confirmed that the size is the key cause of carbon-induced cytotoxicity and it is probably caused by the ATP depletion within the cell.},
doi = {10.1016/j.msec.2016.05.094},
journal = {Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems},
number = C,
volume = 68,
place = {United States},
year = {Wed May 25 00:00:00 EDT 2016},
month = {Wed May 25 00:00:00 EDT 2016}
}
Web of Science
Works referenced in this record:
Pulmonary toxicity of carbon nanotubes: a systematic report
journal, February 2011
- Kayat, Jitendra; Gajbhiye, Virendra; Tekade, Rakesh Kumar
- Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 7, Issue 1
Sustainable Mesoporous Carbons as Storage and Controlled-Delivery Media for Functional Molecules
journal, June 2013
- Saha, Dipendu; Payzant, E. Andrew; Kumbhar, Amar S.
- ACS Applied Materials & Interfaces, Vol. 5, Issue 12
Oxidative stress and inflammatory response in dermal toxicity of single-walled carbon nanotubes
journal, March 2009
- Murray, A. R.; Kisin, E.; Leonard, S. S.
- Toxicology, Vol. 257, Issue 3
Respiratory toxicity of multi-wall carbon nanotubes
journal, September 2005
- Muller, Julie; Huaux, François; Moreau, Nicolas
- Toxicology and Applied Pharmacology, Vol. 207, Issue 3
Respiratory toxicity of carbon nanotubes: How worried should we be?
journal, May 2006
- Muller, Julie; Huaux, François; Lison, Dominique
- Carbon, Vol. 44, Issue 6
Repeated pulmonary exposure to single-walled carbon nanotubes exacerbates allergic inflammation of the airway: Possible role of oxidative stress
journal, April 2010
- Inoue, Ken-ichiro; Yanagisawa, Rie; Koike, Eiko
- Free Radical Biology and Medicine, Vol. 48, Issue 7
The degree and kind of agglomeration affect carbon nanotube cytotoxicity
journal, January 2007
- Wick, P.; Manser, P.; Limbach, L.
- Toxicology Letters, Vol. 168, Issue 2
The biocompatibility of carbon nanotubes
journal, May 2006
- Smart, S. K.; Cassady, A. I.; Lu, G. Q.
- Carbon, Vol. 44, Issue 6
What is currently known about the health risks related to carbon nanotube exposures?
journal, May 2006
- Warheit, D. B.
- Carbon, Vol. 44, Issue 6
Toxicology of carbon nanomaterials: Status, trends, and perspectives on the special issue
journal, May 2006
- Hurt, Robert H.; Monthioux, Marc; Kane, Agnes
- Carbon, Vol. 44, Issue 6
Functionalization density dependence of single-walled carbon nanotubes cytotoxicity in vitro
journal, February 2006
- Sayes, Christie M.; Liang, Feng; Hudson, Jared L.
- Toxicology Letters, Vol. 161, Issue 2
Cell entry of one-dimensional nanomaterials occurs by tip recognition and rotation
journal, September 2011
- Shi, Xinghua; von dem Bussche, Annette; Hurt, Robert H.
- Nature Nanotechnology, Vol. 6, Issue 11
Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites
journal, July 2013
- Li, Y.; Yuan, H.; von dem Bussche, A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 30
Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis
journal, December 2012
- Riding, Matthew J.; Trevisan, Júlio; Hirschmugl, Carol J.
- Environment International, Vol. 50
Hemocompatibility and Macrophage Response of Pristine and Functionalized Graphene
journal, February 2012
- Sasidharan, Abhilash; Panchakarla, Leela S.; Sadanandan, Aparna R.
- Small, Vol. 8, Issue 8
Position Statement: Single-Dose Activated Charcoal
journal, January 1997
- ,
- Journal of Toxicology: Clinical Toxicology, Vol. 35, Issue 7, p. 721-741
Soft-templated mesoporous carbons as potential materials for oral drug delivery
journal, May 2014
- Saha, Dipendu; Warren, Kaitlyn E.; Naskar, Amit K.
- Carbon, Vol. 71
Controlled release of antipyrine from mesoporous carbons
journal, September 2014
- Saha, Dipendu; Warren, Kaitlyn E.; Naskar, Amit K.
- Microporous and Mesoporous Materials, Vol. 196
Micro-/mesoporous carbons for controlled release of antipyrine and indomethacin
journal, January 2015
- Saha, Dipendu; Moken, Tara; Chen, Jihua
- RSC Advances, Vol. 5, Issue 30
Novel synthesis of Fe-containing mesoporous carbons and their release of ibuprofen
journal, November 2011
- Wang, Xiufang; Liu, Ping; Tian, Yong
- Microporous and Mesoporous Materials, Vol. 145, Issue 1-3
Development of new drug delivery system based on ordered mesoporous carbons: characterisation and cytocompatibility studies
journal, January 2013
- Karavasili, Christina; Amanatiadou, Elsa P.; Sygellou, Lambrini
- Journal of Materials Chemistry B, Vol. 1, Issue 25
Slow release kinetics of mitoxantrone from ordered mesoporous carbon films
journal, September 2012
- Labiano, Alpha; Dai, Mingzhi; Taylor, David
- Microporous and Mesoporous Materials, Vol. 160
Controlled release of alendronate from nitrogen-doped mesoporous carbon
journal, July 2016
- Saha, Dipendu; Spurri, Amanda; Chen, Jihua
- Microporous and Mesoporous Materials, Vol. 229
Phospholipid-stabilized mesoporous carbon nanospheres as versatile carriers for systemic delivery of amphiphobic SNX-2112 (a Hsp90 inhibitor) with enhanced antitumor effect
journal, August 2015
- Zhang, Xingwang; Zhang, Tianpeng; Ye, Yanghuan
- European Journal of Pharmaceutics and Biopharmaceutics, Vol. 94
pH-Controlled Delivery of Doxorubicin to Cancer Cells, Based on Small Mesoporous Carbon Nanospheres
journal, June 2012
- Zhu, Jie; Liao, Lei; Bian, Xiaojun
- Small, Vol. 8, Issue 17
Hydrophilic mesoporous carbon nanoparticles as carriers for sustained release of hydrophobic anti-cancer drugs
journal, January 2011
- Gu, Jinlou; Su, Shasha; Li, Yongsheng
- Chem. Commun., Vol. 47, Issue 7
Biocompatibility of Soft-Templated Mesoporous Carbons
journal, August 2014
- Gencoglu, Maria F.; Spurri, Amanda; Franko, Mitchell
- ACS Applied Materials & Interfaces, Vol. 6, Issue 17
Physical approaches to biomaterial design
journal, January 2009
- Mitragotri, Samir; Lahann, Joerg
- Nature Materials, Vol. 8, Issue 1
Pure-Silica Zeolites (Porosils) as Model Solids for the Evaluation of the Physicochemical Features Determining Silica Toxicity to Macrophages
journal, May 2000
- Fenoglio, Ivana; Croce, Antonietta; Di Renzo, Francesco
- Chemical Research in Toxicology, Vol. 13, Issue 6
In vitro cytotoxicity of porous silicon microparticles: Effect of the particle concentration, surface chemistry and size
journal, July 2010
- Santos, Hélder A.; Riikonen, Joakim; Salonen, Jarno
- Acta Biomaterialia, Vol. 6, Issue 7
A Human Colonic Cell Line Sharing Similarities With Enterocytes as a Model to Examine Oral Absorption: Advantages and Limitations of the Caco-2 Model
journal, January 1997
- Delie, Florence; Rubas, Werner
- Critical Reviews in Therapeutic Drug Carrier Systems, Vol. 14, Issue 3
Tetrahydrofuran-Induced K and Li Doping onto Poly(furfuryl alcohol)-Derived Activated Carbon (PFAC): Influence on Microstructure and H 2 Sorption Properties
journal, March 2012
- Saha, Dipendu; Contescu, Cristian I.; Gallego, Nidia C.
- Langmuir, Vol. 28, Issue 13
Cytotoxicity study of ordered mesoporous silica MCM-41 and SBA-15 microparticles on Caco-2 cells
journal, March 2010
- Heikkilä, Teemu; Santos, Hélder A.; Kumar, Narendra
- European Journal of Pharmaceutics and Biopharmaceutics, Vol. 74, Issue 3
Surface characterization of electrochemically oxidized carbon fibers
journal, January 1999
- Yue, Z. R.; Jiang, W.; Wang, L.
- Carbon, Vol. 37, Issue 11
Induction of mild intracellular redox imbalance inhibits proliferation of CaCo‐2 cells
journal, October 2001
- Noda, Takahiro; Iwakiri, Ryuich; Fujimoto, Kazuma
- The FASEB Journal, Vol. 15, Issue 12
Different sensitivities of human colon adenocarcinoma (CaCo-2), astrocytoma (IPDDC-A2) and lymphoblastoid (NCNC) cell lines to microcystin-LR induced reactive oxygen species and DNA damage
journal, September 2008
- Žegura, Bojana; Volčič, Meta; Lah, Tamara T.
- Toxicon, Vol. 52, Issue 3
An in vitro study of the potential of carbon nanotubes and nanofibres to induce inflammatory mediators and frustrated phagocytosis
journal, August 2007
- Brown, D. M.; Kinloch, I. A.; Bangert, U.
- Carbon, Vol. 45, Issue 9
Reactive oxygen species in living systems: Source, biochemistry, and role in human disease
journal, September 1991
- Halliwell, Barry
- The American Journal of Medicine, Vol. 91, Issue 3
DNA damage and oxygen radical toxicity
journal, June 1988
- Imlay, J.; Linn, S.
- Science, Vol. 240, Issue 4857
Works referencing / citing this record:
Recent advancements in Fe-based biodegradable materials for bone repair
journal, October 2018
- Gorejová, R.; Haverová, L.; Oriňaková, R.
- Journal of Materials Science, Vol. 54, Issue 3