Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

Akinosho, Hannah O.; Yoo, Chang Geun; Dumitrache, Alexandru; Natzke, Jace; Muchero, Wellington; Brown, Steven D.; Ragauskas, Arthur J.

doi:10.1021/acssuschemeng.7b01203

Title: Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

Journal Article · 20 July 2017 · ACS Sustainable Chemistry & Engineering

DOI: https://doi.org/10.1021/acssuschemeng.7b01203 · OSTI ID:1394362

Akinosho, Hannah O. ^[1]; Yoo, Chang Geun ^[2]; Dumitrache, Alexandru ^[2]; Natzke, Jace ^[2]; Muchero, Wellington ^[2]; Brown, Steven D. ^[2];

^[3]

Georgia Inst. of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this paper, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. Finally, this investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Texas A&M AgriLife Research, College Station, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC05-00OR22725; EE0007104

OSTI ID:: 1394362

Alternate ID(s):: OSTI ID: 2532437

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 9; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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