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Title: Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this paper, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. Finally, this investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [2] ; ORCiD logo [3]
  1. Georgia Inst. of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
ACS Sustainable Chemistry & Engineering
Additional Journal Information:
Journal Volume: 5; Journal Issue: 9; Journal ID: ISSN 2168-0485
Publisher:
American Chemical Society (ACS)
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Clostridium thermocellum; consolidated bioprocessing; FTIR; lignin characterization; NMR
OSTI Identifier:
1394362

Akinosho, Hannah O., Yoo, Chang Geun, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Brown, Steven D., and Ragauskas, Arthur J.. Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum. United States: N. p., Web. doi:10.1021/acssuschemeng.7b01203.
Akinosho, Hannah O., Yoo, Chang Geun, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Brown, Steven D., & Ragauskas, Arthur J.. Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum. United States. doi:10.1021/acssuschemeng.7b01203.
Akinosho, Hannah O., Yoo, Chang Geun, Dumitrache, Alexandru, Natzke, Jace, Muchero, Wellington, Brown, Steven D., and Ragauskas, Arthur J.. 2017. "Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum". United States. doi:10.1021/acssuschemeng.7b01203. https://www.osti.gov/servlets/purl/1394362.
@article{osti_1394362,
title = {Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum},
author = {Akinosho, Hannah O. and Yoo, Chang Geun and Dumitrache, Alexandru and Natzke, Jace and Muchero, Wellington and Brown, Steven D. and Ragauskas, Arthur J.},
abstractNote = {During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this paper, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. Finally, this investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.},
doi = {10.1021/acssuschemeng.7b01203},
journal = {ACS Sustainable Chemistry & Engineering},
number = 9,
volume = 5,
place = {United States},
year = {2017},
month = {7}
}