Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system
Abstract
The organization of the B. cellulosolvens cellulosome is unique compared to previously described cellulosome systems. In contrast to all other known cellulosomes, the cohesin types are reversed for all scaffoldins i.e., the type II cohesins are located on the enzyme-integrating primary scaffoldin, whereas the type I cohesins are located on the anchoring scaffoldins. Many of the type II dockerin-bearing ORFs include X60 modules, which are known to stabilize type II cohesin–dockerin interactions. In the present work, we focused on revealing the architectural arrangement of cellulosome structure in this bacterium by examining numerous interactions between the various cohesin and dockerin modules. In total, we cloned and expressed 43 representative cohesins and 27 dockerins. The results revealed various possible architectures of cell-anchored and cell-free cellulosomes, which serve to assemble distinctive cellulosome types via three distinct cohesin–dockerin specificities: type I, type II, and a novel-type designated R (distinct from type III interactions, predominant in ruminococcal cellulosomes). The results of this study provide novel insight into the architecture and function of the most intricate and extensive cellulosomal system known today, thereby extending significantly our overall knowledge base of cellulosome systems and their components. The robust cellulosome system of B. cellulosolvens, with its unique bindingmore »
- Authors:
-
[1]
- Weizmann Inst. of Science, Rehovot (Israel). Dept. of Biomolecular Sciences
- Univ. of Tennessee, Knoxville, TN (United States). Graduate School of Genome Science and Technology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Univ. of Tennessee, Knoxville, TN (United States). Graduate School of Genome Science and Technology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, Energy and Environment Directorate
- Aix-Marseille Univ., and CNRS/IN2P3, Marseille (France). Architecture et Fonction des Macromolecules Biologiques
- Tel Aviv Univ., Ramat Aviv (Israel). Dept. of Molecular Microbiology and Biotechnology
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Israel Science Foundation (ISF); United States-Israel Binational Science Foundation (BSF); National Natural Science Foundation of China (NSFC)
- OSTI Identifier:
- 1394357
- Grant/Contract Number:
- AC05-00OR22725; 2566/16; 1349
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; cohesion; dockerin; scaffoldin; cellulolytic bacteria; CBM; cellulases; Glgycoside hydrolases
Citation Formats
Zhivin, Olga, Dassa, Bareket, Moraïs, Sarah, Utturkar, Sagar M., Brown, Steven D., Henrissat, Bernard, Lamed, Raphael, and Bayer, Edward A. Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system. United States: N. p., 2017.
Web. doi:10.1186/s13068-017-0898-6.
Zhivin, Olga, Dassa, Bareket, Moraïs, Sarah, Utturkar, Sagar M., Brown, Steven D., Henrissat, Bernard, Lamed, Raphael, & Bayer, Edward A. Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system. United States. https://doi.org/10.1186/s13068-017-0898-6
Zhivin, Olga, Dassa, Bareket, Moraïs, Sarah, Utturkar, Sagar M., Brown, Steven D., Henrissat, Bernard, Lamed, Raphael, and Bayer, Edward A. Thu .
"Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system". United States. https://doi.org/10.1186/s13068-017-0898-6. https://www.osti.gov/servlets/purl/1394357.
@article{osti_1394357,
title = {Unique organization and unprecedented diversity of the Bacteroides (Pseudobacteroides) cellulosolvens cellulosome system},
author = {Zhivin, Olga and Dassa, Bareket and Moraïs, Sarah and Utturkar, Sagar M. and Brown, Steven D. and Henrissat, Bernard and Lamed, Raphael and Bayer, Edward A.},
abstractNote = {The organization of the B. cellulosolvens cellulosome is unique compared to previously described cellulosome systems. In contrast to all other known cellulosomes, the cohesin types are reversed for all scaffoldins i.e., the type II cohesins are located on the enzyme-integrating primary scaffoldin, whereas the type I cohesins are located on the anchoring scaffoldins. Many of the type II dockerin-bearing ORFs include X60 modules, which are known to stabilize type II cohesin–dockerin interactions. In the present work, we focused on revealing the architectural arrangement of cellulosome structure in this bacterium by examining numerous interactions between the various cohesin and dockerin modules. In total, we cloned and expressed 43 representative cohesins and 27 dockerins. The results revealed various possible architectures of cell-anchored and cell-free cellulosomes, which serve to assemble distinctive cellulosome types via three distinct cohesin–dockerin specificities: type I, type II, and a novel-type designated R (distinct from type III interactions, predominant in ruminococcal cellulosomes). The results of this study provide novel insight into the architecture and function of the most intricate and extensive cellulosomal system known today, thereby extending significantly our overall knowledge base of cellulosome systems and their components. The robust cellulosome system of B. cellulosolvens, with its unique binding specificities and reversal of cohesin–dockerin types, has served to amend our view of the cellulosome paradigm. Revealing new cellulosomal interactions and arrangements is critical for designing high-efficiency artificial cellulosomes for conversion of plant-derived cellulosic biomass towards improved production of biofuels.},
doi = {10.1186/s13068-017-0898-6},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {9}
}
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