Molybdenum-Based Diazotrophy in a Sphagnum Peatland in Northern Minnesota
Abstract
Microbial N2fixation (diazotrophy) represents an important nitrogen source to oligotrophic peatland ecosystems, which are important sinks for atmospheric CO2 and are susceptible to the changing climate. The objectives of this study were (i) to determine the active microbial group and type of nitrogenase mediating diazotrophy in an ombrotrophicSphagnum-dominated peat bog (the S1 peat bog, Marcell Experimental Forest, Minnesota, USA); and (ii) to determine the effect of environmental parameters (light, O2, CO2, and CH4) on potential rates of diazotrophy measured by acetylene (C2H2) reduction and15N2incorporation. A molecular analysis of metabolically active microbial communities suggested that diazotrophy in surface peat was primarily mediated byAlphaproteobacteria(BradyrhizobiaceaeandBeijerinckiaceae). Despite higher concentrations of dissolved vanadium ([V] 11 nM) than molybdenum ([Mo] 3 nM) in surface peat, a combination of metagenomic, amplicon sequencing, and activity measurements indicated that Mo-containing nitrogenases dominate over the V-containing form. Acetylene reduction was only detected in surface peat exposed to light, with the highest rates observed in peat collected from hollows with the highest water contents. Incorporation of15N2was suppressed 90% by O2and 55% by C2H2and was unaffected by CH4and CO2amendments. These results suggest that peatland diazotrophy is mediated by a combination of C2H2-sensitive and C2H2-insensitive microbes that are more active at low concentrationsmore »
- Authors:
-
[6]
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Earth and Atmospheric Sciences
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Biological Sciences
- Florida State Univ., Tallahassee, FL (United States). National High Magnetic Field Lab. (MagLab)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical and Life Sciences Directorate
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Divison
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Earth and Atmospheric Sciences and School of Biological Sciences
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1394354
- Alternate Identifier(s):
- OSTI ID: 1478664
- Report Number(s):
- LLNL-JRNL-731618
Journal ID: ISSN 0099-2240
- Grant/Contract Number:
- AC05-00OR22725; SC0007144; SC0012088; AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: 17; Journal ID: ISSN 0099-2240
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Alphaproteobacteria; Sphagnum; acetylene; diazotrophy; methanotrophs; molybdenum; nitrogen cycle enzymes; nitrogen fixation; peatland; vanadium
Citation Formats
Warren, Melissa J., Lin, Xueju, Gaby, John C., Kretz, Cecilia B., Kolton, Max, Morton, Peter L., Pett-Ridge, Jennifer, Weston, David J., Schadt, Christopher W., Kostka, Joel E., and Glass, Jennifer B. Molybdenum-Based Diazotrophy in a Sphagnum Peatland in Northern Minnesota. United States: N. p., 2017.
Web. doi:10.1128/AEM.01174-17.
Warren, Melissa J., Lin, Xueju, Gaby, John C., Kretz, Cecilia B., Kolton, Max, Morton, Peter L., Pett-Ridge, Jennifer, Weston, David J., Schadt, Christopher W., Kostka, Joel E., & Glass, Jennifer B. Molybdenum-Based Diazotrophy in a Sphagnum Peatland in Northern Minnesota. United States. https://doi.org/10.1128/AEM.01174-17
Warren, Melissa J., Lin, Xueju, Gaby, John C., Kretz, Cecilia B., Kolton, Max, Morton, Peter L., Pett-Ridge, Jennifer, Weston, David J., Schadt, Christopher W., Kostka, Joel E., and Glass, Jennifer B. Fri .
"Molybdenum-Based Diazotrophy in a Sphagnum Peatland in Northern Minnesota". United States. https://doi.org/10.1128/AEM.01174-17. https://www.osti.gov/servlets/purl/1394354.
@article{osti_1394354,
title = {Molybdenum-Based Diazotrophy in a Sphagnum Peatland in Northern Minnesota},
author = {Warren, Melissa J. and Lin, Xueju and Gaby, John C. and Kretz, Cecilia B. and Kolton, Max and Morton, Peter L. and Pett-Ridge, Jennifer and Weston, David J. and Schadt, Christopher W. and Kostka, Joel E. and Glass, Jennifer B.},
abstractNote = {Microbial N2fixation (diazotrophy) represents an important nitrogen source to oligotrophic peatland ecosystems, which are important sinks for atmospheric CO2 and are susceptible to the changing climate. The objectives of this study were (i) to determine the active microbial group and type of nitrogenase mediating diazotrophy in an ombrotrophicSphagnum-dominated peat bog (the S1 peat bog, Marcell Experimental Forest, Minnesota, USA); and (ii) to determine the effect of environmental parameters (light, O2, CO2, and CH4) on potential rates of diazotrophy measured by acetylene (C2H2) reduction and15N2incorporation. A molecular analysis of metabolically active microbial communities suggested that diazotrophy in surface peat was primarily mediated byAlphaproteobacteria(BradyrhizobiaceaeandBeijerinckiaceae). Despite higher concentrations of dissolved vanadium ([V] 11 nM) than molybdenum ([Mo] 3 nM) in surface peat, a combination of metagenomic, amplicon sequencing, and activity measurements indicated that Mo-containing nitrogenases dominate over the V-containing form. Acetylene reduction was only detected in surface peat exposed to light, with the highest rates observed in peat collected from hollows with the highest water contents. Incorporation of15N2was suppressed 90% by O2and 55% by C2H2and was unaffected by CH4and CO2amendments. These results suggest that peatland diazotrophy is mediated by a combination of C2H2-sensitive and C2H2-insensitive microbes that are more active at low concentrations of O2and show similar activity at high and low concentrations of CH4. IMPORTANCE Previous studies indicate that diazotrophy provides an important nitrogen source and is linked to methanotrophy inSphagnum-dominated peatlands. However, the environmental controls and enzymatic pathways of peatland diazotrophy, as well as the metabolically active microbial populations that catalyze this process, remain in question. Our findings indicate that oxygen levels and photosynthetic activity override low nutrient availability in limiting diazotrophy and that members of theAlphaproteobacteria(Rhizobiales) catalyze this process at the bog surface using the molybdenum-based form of the nitrogenase enzyme.},
doi = {10.1128/AEM.01174-17},
journal = {Applied and Environmental Microbiology},
number = 17,
volume = 83,
place = {United States},
year = {Fri Jun 30 00:00:00 EDT 2017},
month = {Fri Jun 30 00:00:00 EDT 2017}
}
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Vertical Variation of Nitrogen Fixers and Ammonia Oxidizers along a Sediment Profile in the Dajiuhu Peatland, Central China
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Experimental warming alters the community composition, diversity, and N 2 fixation activity of peat moss ( Sphagnum fallax ) microbiomes
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The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses
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