Conformational landscape of a virus by single-particle X-ray scattering
- Univ. of Wisconsin, Milwaukee, WI (United States)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Arizona State Univ., Tempe, AZ (United States)
- Brookhaven National Lab. (BNL), Upton, NY (United States)
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. Furthermore, these results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.
- Research Organization:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Univ. of Wisconsin, Milwaukee, WI (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- Grant/Contract Number:
- AC02-76SF00515; SC0002164
- OSTI ID:
- 1394091
- Alternate ID(s):
- OSTI ID: 1598200
- Journal Information:
- Nature Methods, Vol. 14, Issue 9; ISSN 1548-7091
- Publisher:
- Nature Publishing GroupCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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