Conformational landscape of a virus by single-particle X-ray scattering

Hosseinizadeh, Ahmad; Mashayekhi, Ghoncheh; Copperman, Jeremy; Schwander, Peter; Dashti, Ali; Sepehr, Reyhaneh; Fung, Russell; Schmidt, Marius; Yoon, Chun Hong; Hogue, Brenda G.; Williams, Garth J.; Aquila, Andrew; Ourmazd, Abbas

doi:10.1038/NMETH.4395

Title: Conformational landscape of a virus by single-particle X-ray scattering

Journal Article · 14 August 2017 · Nature Methods

DOI: https://doi.org/10.1038/NMETH.4395 · OSTI ID:1394091

Hosseinizadeh, Ahmad ^[1]; Mashayekhi, Ghoncheh ^[1]; Copperman, Jeremy ^[1]; Schwander, Peter ^[1]; Dashti, Ali ^[1]; Sepehr, Reyhaneh ^[1]; Fung, Russell ^[1]; Schmidt, Marius ^[1]; Yoon, Chun Hong ^[2]; Hogue, Brenda G. ^[3]; Williams, Garth J. ^[4]; Aquila, Andrew ^[2];

^[1]

Univ. of Wisconsin, Milwaukee, WI (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Arizona State Univ., Tempe, AZ (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)

Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. Furthermore, these results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Univ. of Wisconsin, Milwaukee, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515; SC0002164

OSTI ID:: 1394091

Alternate ID(s):: OSTI ID: 1598200

Journal Information:: Nature Methods, Vol. 14, Issue 9; ISSN 1548-7091

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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