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Title: Conformational landscape of a virus by single-particle X-ray scattering

Abstract

Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. Furthermore, these results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [4];  [2]; ORCiD logo [1]
  1. Univ. of Wisconsin, Milwaukee, WI (United States)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Arizona State Univ., Tempe, AZ (United States)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Univ. of Wisconsin, Milwaukee, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1394091
Alternate Identifier(s):
OSTI ID: 1598200
Grant/Contract Number:  
AC02-76SF00515; SC0002164
Resource Type:
Accepted Manuscript
Journal Name:
Nature Methods
Additional Journal Information:
Journal Volume: 14; Journal Issue: 9; Journal ID: ISSN 1548-7091
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 97 MATHEMATICS AND COMPUTING

Citation Formats

Hosseinizadeh, Ahmad, Mashayekhi, Ghoncheh, Copperman, Jeremy, Schwander, Peter, Dashti, Ali, Sepehr, Reyhaneh, Fung, Russell, Schmidt, Marius, Yoon, Chun Hong, Hogue, Brenda G., Williams, Garth J., Aquila, Andrew, and Ourmazd, Abbas. Conformational landscape of a virus by single-particle X-ray scattering. United States: N. p., 2017. Web. https://doi.org/10.1038/NMETH.4395.
Hosseinizadeh, Ahmad, Mashayekhi, Ghoncheh, Copperman, Jeremy, Schwander, Peter, Dashti, Ali, Sepehr, Reyhaneh, Fung, Russell, Schmidt, Marius, Yoon, Chun Hong, Hogue, Brenda G., Williams, Garth J., Aquila, Andrew, & Ourmazd, Abbas. Conformational landscape of a virus by single-particle X-ray scattering. United States. https://doi.org/10.1038/NMETH.4395
Hosseinizadeh, Ahmad, Mashayekhi, Ghoncheh, Copperman, Jeremy, Schwander, Peter, Dashti, Ali, Sepehr, Reyhaneh, Fung, Russell, Schmidt, Marius, Yoon, Chun Hong, Hogue, Brenda G., Williams, Garth J., Aquila, Andrew, and Ourmazd, Abbas. Mon . "Conformational landscape of a virus by single-particle X-ray scattering". United States. https://doi.org/10.1038/NMETH.4395. https://www.osti.gov/servlets/purl/1394091.
@article{osti_1394091,
title = {Conformational landscape of a virus by single-particle X-ray scattering},
author = {Hosseinizadeh, Ahmad and Mashayekhi, Ghoncheh and Copperman, Jeremy and Schwander, Peter and Dashti, Ali and Sepehr, Reyhaneh and Fung, Russell and Schmidt, Marius and Yoon, Chun Hong and Hogue, Brenda G. and Williams, Garth J. and Aquila, Andrew and Ourmazd, Abbas},
abstractNote = {Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. Furthermore, these results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.},
doi = {10.1038/NMETH.4395},
journal = {Nature Methods},
number = 9,
volume = 14,
place = {United States},
year = {2017},
month = {8}
}

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Cited by: 17 works
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