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Title: Aligning the Measurement of Microbial Diversity with Macroecological Theory

The number of microbial operational taxonomic units (OTUs) within a community is akin to species richness within plant/animal (‘macrobial’) systems. A large literature documents OTU richness patterns, drawing comparisons to macrobial theory. There is, however, an unrecognized fundamental disconnect between OTU richness and macrobial theory: OTU richness is commonly estimated on a per-individual basis, while macrobial richness is estimated per-area. Furthermore, the range or extent of sampled environmental conditions can strongly influence a study’s outcomes and conclusions, but this is not commonly addressed when studying OTU richness. Here we (i) propose a new sampling approach that estimates OTU richness per-mass of soil, which results in strong support for species energy theory, (ii) use data reduction to show how support for niche conservatism emerges when sampling across a restricted range of environmental conditions, and (iii) show how additional insights into drivers of OTU richness can be generated by combining different sampling methods while simultaneously considering patterns that emerge by restricting the range of environmental conditions. We propose that a more rigorous connection between microbial ecology and macrobial theory can be facilitated by exploring how changes in OTU richness units and environmental extent influence outcomes of data analysis. While fundamental differences betweenmore » microbial and macrobial systems persist (e.g., species concepts), we suggest that closer attention to units and scale provide tangible and immediate improvements to our understanding of the processes governing OTU richness and how those processes relate to drivers of macrobial species richness.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [5] ;  [6] ;  [1] ;  [5] ;  [5]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  2. Univ. of North Carolina, Chapel Hill, NC (United States). Biology Department and Curriculum in Environment and Ecology
  3. Pacific Northwest National Lab. (PNNL), College Park, MD (United States). Joint Global Change Research Institute
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Atmospheric Sciences and Global Change Division
  5. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  6. University of Groningen (The Netherlands). Microbial Ecology Cluster, Genomics Research in Ecology and Evolution in Nature (GREEN), Groningen Institute for Evolutionary Life Sciences (GELIFES)
Publication Date:
Report Number(s):
PNNL-SA-122085
Journal ID: ISSN 1664-302X
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; species richness; OTU richness; Species energy theory; Niche conservatism; rarefaction; Soil; Permafrost; boreal forest
OSTI Identifier:
1393763