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Title: Simulation of outdoor pond cultures using indoor LED-lighted and temperature-controlled raceway ponds and Phenometrics photobioreactors

Two innovative culturing systems, the LED-lighted and temperature-controlled 800 liter indoor raceways at Pacific Northwest National Laboratory (PNNL) and the Phenometrics environmental Photobioreactors™ (ePBRs) were evaluated in terms of their ability to accurately simulate the microalgae growth performance of outdoor cultures subjected to fluctuating sunlight and water temperature conditions. When repeating a 60-day outdoor pond culture experiment (batch and semi-continuous at two dilution rates) conducted in Arizona with the freshwater strain Chlorella sorokiniana DOE 1412 in these two indoor simulators, it was found that ash-free dry weight based biomass growth and productivity in the PNNL climate-simulation ponds was comparatively slightly higher (8–13%) but significantly lower (44%) in the ePBRs. The difference in biomass productivities between the indoor and outdoor ponds was not statistically significant. When the marine Picochlorum soloecismus was cultured in five replicate ePBRs at Los Alamos National Laboratory (LANL) and in duplicate indoor climate-simulation ponds at PNNL, using the same inoculum, medium, culture depth, and light and temperature scripts, the optical density based biomass productivity and the rate of increase in cell counts in the ePBRs was about 35% and 66%, respectively, lower compared than in the indoor ponds. Potential reasons for the divergence in growth performance inmore » these pond simulators, relative to outdoor raceways, are discussed. In conclusion, the PNNL climate-simulation ponds provide reasonably reliable biomass productivity estimates for microalgae strains cultured in outdoor raceways under different climatic conditions.« less
 [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [2] ;  [1] ;  [2] ;  [3] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Marine Sciences Lab.
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Hydrology Group
Publication Date:
Report Number(s):
LA-UR-17-21029; PNNL-SA-117542
Journal ID: ISSN 2211-9264
Grant/Contract Number:
EE0003046; AC05-76RL01830
Published Article
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 21; Journal ID: ISSN 2211-9264
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
59 BASIC BIOLOGICAL SCIENCES; Phenometrics photobioreactors; ePBR; climate-simulation raceway ponds; Chlorella sorokiniana; Picochlorum soloecismus; flow cytometry; climate-simulation ponds; indoor raceways; Picochlorum sp.; cell sorting
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1357134; OSTI ID: 1361967