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Title: Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems

A long-standing desire in biological and biomedical sciences is to be able to probe cellular chemistry as biological processes are happening inside living cells. Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy is a label-free and nondestructive analytical technique that can provide spatiotemporal distributions and relative abundances of biomolecules of a specimen by their characteristic vibrational modes. Despite great progress in recent years, SR-FTIR imaging of living biological systems remains challenging because of the demanding requirements on environmental control and strong infrared absorption of water. To meet this challenge, microfluidic devices have emerged as a method to control the water thickness while providing a hospitable environment to measure cellular processes and responses over many hours or days. This paper will provide an overview of microfluidic device development for SR-FTIR imaging of living biological systems, provide contrast between the various techniques including closed and open-channel designs, and discuss future directions of development within this area. Even as the fundamental science and technological demonstrations develop, other ongoing issues must be addressed; for example, choosing applications whose experimental requirements closely match device capabilities, and developing strategies to efficiently complete the cycle of development. These will require imagination, ingenuity and collaboration.
Authors:
 [1] ;  [2] ;  [1] ;  [1]
  1. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Berkeley Synchrotron Infrared Structural Biology (BSISB) Program
  2. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Berkeley Synchrotron Infrared Structural Biology (BSISB) Program; Elettra Synchrotron Light Lab., Trieste (Italy)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Protein and Peptide Letters
Additional Journal Information:
Journal Volume: 23; Journal Issue: 3; Journal ID: ISSN 0929-8665
Publisher:
Bentham Science Publishers
Research Org:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Berkeley Synchrotron Infrared Structural Biology (BSISB) Program
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
OSTI Identifier:
1393593