Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion
Abstract
We report on entirely man-made nanobio hybrid fabricated through assembly of cell-free expressed transmembrane proton pump and semiconductor nanoparticles as an efficient nanocatalysis for photocatalytic H2 evolution. The system produces H2 at a turnover rate of 239 (μmole protein)-1 h-1 under green and 17742 (μmole protein)-1 h-1 under white light at ambient conditions, in water at neutral pH with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allows for systemic manipulation at nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.
- Authors:
-
[5];
[6]
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials; Shandong Univ. (China). Key Laboratory of Crystal Materials
- Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry
- Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Div.
- Moscow Institute of Physics and Technology, Dolgoprudny, Moscow (Russian Federation). Laboratory Chemistry and Physics of Lipids, Department of General and Applied Physics
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials; Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry
- Argonne National Lab. (ANL), Argonne, IL (United States). Center for Nanoscale Materials
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Basic Research Program of China; Ministry of Education and Science of the Russian Federation; USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1393574
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Nano
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 7; Journal ID: ISSN 1936-0851
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; TiO2; cell-free; hydrogen; nanoparticles; solar-to-fuel conversion; synthetic biology
Citation Formats
Wang, Peng, Chang, Angela Y., Novosad, Valentyn, Chupin, Vladimir V., Schaller, Richard D., and Rozhkova, Elena A. Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. United States: N. p., 2017.
Web. doi:10.1021/acsnano.7b01142.
Wang, Peng, Chang, Angela Y., Novosad, Valentyn, Chupin, Vladimir V., Schaller, Richard D., & Rozhkova, Elena A. Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion. United States. https://doi.org/10.1021/acsnano.7b01142
Wang, Peng, Chang, Angela Y., Novosad, Valentyn, Chupin, Vladimir V., Schaller, Richard D., and Rozhkova, Elena A. Sun .
"Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion". United States. https://doi.org/10.1021/acsnano.7b01142. https://www.osti.gov/servlets/purl/1393574.
@article{osti_1393574,
title = {Cell-Free Synthetic Biology Chassis for Nanocatalytic Photon-to-Hydrogen Conversion},
author = {Wang, Peng and Chang, Angela Y. and Novosad, Valentyn and Chupin, Vladimir V. and Schaller, Richard D. and Rozhkova, Elena A.},
abstractNote = {We report on entirely man-made nanobio hybrid fabricated through assembly of cell-free expressed transmembrane proton pump and semiconductor nanoparticles as an efficient nanocatalysis for photocatalytic H2 evolution. The system produces H2 at a turnover rate of 239 (μmole protein)-1 h-1 under green and 17742 (μmole protein)-1 h-1 under white light at ambient conditions, in water at neutral pH with methanol as a sacrificial electron donor. Robustness and flexibility of this approach allows for systemic manipulation at nanoparticle-bio interface toward directed evolution of energy transformation materials and artificial systems.},
doi = {10.1021/acsnano.7b01142},
journal = {ACS Nano},
number = 7,
volume = 11,
place = {United States},
year = {Sun Jun 11 00:00:00 EDT 2017},
month = {Sun Jun 11 00:00:00 EDT 2017}
}
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