Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins

Abstract

Background.The cell wall polymer lignin provides structural support and rigidity to plant cell walls, and therefore to the plant body. However, the recalcitrance associated with lignin impedes the extraction of polysaccharides from the cell wall to make plant-based biofuels and biomaterials. The cell wall digestibility can be improved by introducing labile ester bonds into the lignin backbone that can be easily broken under mild base treatment at room temperature. The FERULOYL-CoA MONOLIGNOL TRANSFERASE (FMT) enzyme, which may be naturally found in many plants, uses feruloyl-CoA and monolignols to synthesize the ester-linked monolignol ferulate conjugates. A mutation in the first lignin-specific biosynthetic enzyme, CINNAMOYL-CoA REDUCTASE (CCR), results in an increase in the intracellular pool of feruloyl-CoA. Results. Maize (Zea mays) has a native putative FMT enzyme, and its ccr mutants produce an increased pool of feruloyl-CoA that can be used for conversion to monolignol ferulate conjugates. The decreased lignin content and monomers did not, however, impact the plant growth or biomass. The increase in monolignol conjugates correlated with an improvement in the digestibility of maize stem rind tissue. Conclusions. Together, increased monolignol ferulates and improved digestibility in ccr1 mutant plants suggests that they may be superior biofuel crops.

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States). Wisconsin Energy Institute
Sponsoring Org.:
USDOE
OSTI Identifier:
1618676
Alternate Identifier(s):
OSTI ID: 1393539
Grant/Contract Number:  
BER DE-FC02-07ER64494; FC02-07ER64494
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; Zea mays; Mass spectrometry; Cell wall digestibility; Biofuels

Citation Formats

Smith, Rebecca A., Cass, Cynthia L., Mazaheri, Mona, Sekhon, Rajandeep S., Heckwolf, Marlies, Kaeppler, Heidi, de Leon, Natalia, Mansfield, Shawn D., Kaeppler, Shawn M., Sedbrook, John C., Karlen, Steven D., and Ralph, John. Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins. Netherlands: N. p., 2017. Web. doi:10.1186/s13068-017-0793-1.
Copy to clipboard
Smith, Rebecca A., Cass, Cynthia L., Mazaheri, Mona, Sekhon, Rajandeep S., Heckwolf, Marlies, Kaeppler, Heidi, de Leon, Natalia, Mansfield, Shawn D., Kaeppler, Shawn M., Sedbrook, John C., Karlen, Steven D., & Ralph, John. Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins. Netherlands. https://doi.org/10.1186/s13068-017-0793-1
Copy to clipboard
Smith, Rebecca A., Cass, Cynthia L., Mazaheri, Mona, Sekhon, Rajandeep S., Heckwolf, Marlies, Kaeppler, Heidi, de Leon, Natalia, Mansfield, Shawn D., Kaeppler, Shawn M., Sedbrook, John C., Karlen, Steven D., and Ralph, John. Tue . "Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins". Netherlands. https://doi.org/10.1186/s13068-017-0793-1.
Copy to clipboard
@article{osti_1618676,
title = {Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins},
author = {Smith, Rebecca A. and Cass, Cynthia L. and Mazaheri, Mona and Sekhon, Rajandeep S. and Heckwolf, Marlies and Kaeppler, Heidi and de Leon, Natalia and Mansfield, Shawn D. and Kaeppler, Shawn M. and Sedbrook, John C. and Karlen, Steven D. and Ralph, John},
abstractNote = {Background.The cell wall polymer lignin provides structural support and rigidity to plant cell walls, and therefore to the plant body. However, the recalcitrance associated with lignin impedes the extraction of polysaccharides from the cell wall to make plant-based biofuels and biomaterials. The cell wall digestibility can be improved by introducing labile ester bonds into the lignin backbone that can be easily broken under mild base treatment at room temperature. The FERULOYL-CoA MONOLIGNOL TRANSFERASE (FMT) enzyme, which may be naturally found in many plants, uses feruloyl-CoA and monolignols to synthesize the ester-linked monolignol ferulate conjugates. A mutation in the first lignin-specific biosynthetic enzyme, CINNAMOYL-CoA REDUCTASE (CCR), results in an increase in the intracellular pool of feruloyl-CoA. Results. Maize (Zea mays) has a native putative FMT enzyme, and its ccr mutants produce an increased pool of feruloyl-CoA that can be used for conversion to monolignol ferulate conjugates. The decreased lignin content and monomers did not, however, impact the plant growth or biomass. The increase in monolignol conjugates correlated with an improvement in the digestibility of maize stem rind tissue. Conclusions. Together, increased monolignol ferulates and improved digestibility in ccr1 mutant plants suggests that they may be superior biofuel crops.},
doi = {10.1186/s13068-017-0793-1},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Tue May 02 00:00:00 EDT 2017},
month = {Tue May 02 00:00:00 EDT 2017}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1186/s13068-017-0793-1
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 28 works
Citation information provided by
Web of Science

Figures / Tables:

Figure 1 Figure 1: An abbreviated version of the lignin biosynthetic pathway highlighting the effects of the ccrl mutation.The mutated CCR/ gene results in reduced CCR/ transcript, and presumably less CCRl enzyme, and a pool offeruloyl-CoA (FA reserv oir).The conjugates formed between the monol­ ignols and feruloyl-CoA by ZmFMT are incorporated intomore » the lignin polymer, generating a zip-ligni n in which the ester bonds in the backbone are readily cleaved by base« less
All figures and tables (7 total)
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Redirection of the phenylpropanoid pathway to feruloyl malate in Arabidopsis mutants deficient for cinnamoyl-CoA reductase 1
journal, November 2007

Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
journal, January 2012

  • Withers, Saunia; Lu, Fachuang; Kim, Hoon
  • Journal of Biological Chemistry, Vol. 287, Issue 11, p. 8347-8355
  • DOI: 10.1074/jbc.M111.284497

Characterization of a cinnamoyl-CoA reductase 1 (CCR1) mutant in maize: effects on lignification, fibre development, and global gene expression
journal, April 2011

  • Tamasloukht, Barek; Wong Quai Lam, Mary Sarah-Jane; Martinez, Yves
  • Journal of Experimental Botany, Vol. 62, Issue 11
  • DOI: 10.1093/jxb/err077

Steady-state transposon mutagenesis in inbred maize: Maize steady-state transposon mutagenesis
journal, September 2005

Detection and Determination of p-Coumaroylated Units in Lignins
journal, May 1999

  • Lu, Fachuang; Ralph, John
  • Journal of Agricultural and Food Chemistry, Vol. 47, Issue 5, p. 1988-1992
  • DOI: 10.1021/jf981140j

Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014

p -Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon
journal, February 2014

  • Petrik, Deborah L.; Karlen, Steven D.; Cass, Cynthia L.
  • The Plant Journal, Vol. 77, Issue 5
  • DOI: 10.1111/tpj.12420

Monolignol ferulate conjugates are naturally incorporated into plant lignins
journal, October 2016

  • Karlen, Steven D.; Zhang, Chengcheng; Peck, Matthew L.
  • Science Advances, Vol. 2, Issue 10
  • DOI: 10.1126/sciadv.1600393

Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
journal, November 2007

  • Leplé, Jean-Charles; Dauwe, Rebecca; Morreel, Kris
  • The Plant Cell, Vol. 19, Issue 11
  • DOI: 10.1105/tpc.107.054148

Identification and suppression of the p‐ coumaroyl CoA:hydroxycinnamyl alcohol transferase in Zea mays L.
journal, May 2014

  • Marita, Jane M.; Hatfield, Ronald D.; Rancour, David M.
  • The Plant Journal, Vol. 78, Issue 5
  • DOI: 10.1111/tpj.12510

A High-Throughput Platform for Screening Milligram Quantities of Plant Biomass for Lignocellulose Digestibility
journal, January 2010

  • Santoro, Nicholas; Cantu, Shane L.; Tornqvist, Carl-Erik
  • BioEnergy Research, Vol. 3, Issue 1
  • DOI: 10.1007/s12155-009-9074-6

The DFRC Method for Lignin Analysis. 2. Monomers from Isolated Lignins
journal, February 1998

  • Lu, Fachuang; Ralph, John
  • Journal of Agricultural and Food Chemistry, Vol. 46, Issue 2
  • DOI: 10.1021/jf970676m

DFRC Method for Lignin Analysis. 1. New Method for β-Aryl Ether Cleavage:  Lignin Model Studies
journal, December 1997

  • Lu, Fachuang; Ralph, John
  • Journal of Agricultural and Food Chemistry, Vol. 45, Issue 12
  • DOI: 10.1021/jf970539p

Effect of initial moisture content and chip size on the bioconversion efficiency of softwood lignocellulosics
journal, January 2004

  • Cullis, Ian F.; Saddler, John N.; Mansfield, Shawn D.
  • Biotechnology and Bioengineering, Vol. 85, Issue 4
  • DOI: 10.1002/bit.10905

The DFRC Method for Lignin Analysis. Part 3. NMR Studies
journal, May 1998

Cloning and characterization of irregular xylem4 (irx4):a severely lignin-deficient mutant of Arabidopsis
journal, April 2001

Coniferyl Ferulate Incorporation into Lignin Enhances the Alkaline Delignification and Enzymatic Degradation of Cell Walls
journal, September 2008

  • Grabber, John H.; Hatfield, Ronald D.; Lu, Fachuang
  • Biomacromolecules, Vol. 9, Issue 9, p. 2510-2516
  • DOI: 10.1021/bm800528f
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    The lignin toolbox of the model grass Setaria viridis
    journal, June 2019

    • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
    • Plant Molecular Biology, Vol. 101, Issue 3
    • DOI: 10.1007/s11103-019-00897-9

    Reductive Cleavage Method for Quantitation of Monolignols and Low-Abundance Monolignol Conjugates
    journal, May 2018

    • Regner, Matt; Bartuce, Allison; Padmakshan, Dharshana
    • ChemSusChem, Vol. 11, Issue 10
    • DOI: 10.1002/cssc.201800617
      Sort by:
      [ × clear filter / sort ]

      Figures / Tables found in this record:

      Figure 1 (p. 3)figure
      Figure 2 (p. 4)figure
      Table 1 (p. 5)table
      Table 2 (p. 5)table
      Figure 3 (p. 6)figure
      Figure 4 (p. 7)figure
      Table 3 (p. 7)table
        [ × clear filter / sort ]
        Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

        Similar Records in DOE PAGES and OSTI.GOV collections:

        Other research related to this record: