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Title: A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize

Abstract

Aerial plant organs possess a diverse array of extracellular surface lipids, including both non-polar and amphipathic constituents that collectively provide a primary line of defense against environmental stressors. Extracellular surface lipids on the stigmatic silks of maize are composed primarily of saturated and unsaturated linear hydrocarbons, as well as fatty acids, and aldehydes. To efficiently extract lipids of differing polarities from maize silks, five solvent systems (hexanes; hexanes:diethyl ether (95:5); hexanes:diethyl ether (90:10); chloroform:hexanes (1:1) and chloroform) were tested by immersing fresh silks in solvent for different extraction times. Surface lipid recovery and the relative composition of individual constituents were impacted to varying degrees depending on solvent choice and duration of extraction. Analyses were performed using both silks and leaves to demonstrate the utility of the solvent- and time-optimized protocol in comparison to extraction with the commonly used chloroform solvent. Overall, the preferred solvent system was identified as hexanes:diethyl ether (90:10), based on its effectiveness in extracting surface hydrocarbons and fatty acids as well as its reduced propensity to extract presumed internal fatty acids. Metabolite profiling of wildtype and glossy1 seedlings, which are impaired in surface lipid biosynthesis, demonstrated the ability of the preferred solvent to extract extracellular surface lipidsmore » rich in amphipathic compounds (aldehydes and alcohols). In addition to the expected deficiencies in dotriacontanal and dotriacontan-1-ol for gl1 seedlings, an unexpected increase in fatty acid recovery was observed in gl1 seedlings extracted in chloroform, suggesting that chloro-form extracts lipids from internal tissues of gl1 seedlings. This highlights the importance of extraction method when evaluating mutants that have altered cuticular lipid compositions. Lastly, metabolite profiling of silks from maize inbreds B73 and Mo17, exposed to different environments and harvested at different ages, revealed differences in hydrocarbon and fatty acid composition, demonstrating the dynamic nature of surface lipid accumulation on silks.« less

Authors:
 [1];  [2];  [3];  [2];  [2]; ORCiD logo [3];  [4]
  1. Case Western Reserve Univ., Cleveland, OH (United States); Dept. of Genetics, Development & Cell Biology, Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
  2. Iowa State Univ., Ames, IA (United States)
  3. Dept. of Genetics, Development & Cell Biology, Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
  4. Univ. do Porto (Portugal)
Publication Date:
Research Org.:
Iowa State Univ., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1393507
Grant/Contract Number:  
1354799; 2011-67003-30342
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; lipids; fatty acids; lipid metabolism; hydrocarbons; chloroform; ethers; metabolites; seedlings

Citation Formats

Loneman, Derek M., Peddicord, Layton, Al-Rashid, Amani, Nikolau, Basil J., Lauter, Nick, Yandeau-Nelson, Marna D., and Andrade, Paula B. A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0180850.
Loneman, Derek M., Peddicord, Layton, Al-Rashid, Amani, Nikolau, Basil J., Lauter, Nick, Yandeau-Nelson, Marna D., & Andrade, Paula B. A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize. United States. doi:10.1371/journal.pone.0180850.
Loneman, Derek M., Peddicord, Layton, Al-Rashid, Amani, Nikolau, Basil J., Lauter, Nick, Yandeau-Nelson, Marna D., and Andrade, Paula B. Tue . "A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize". United States. doi:10.1371/journal.pone.0180850. https://www.osti.gov/servlets/purl/1393507.
@article{osti_1393507,
title = {A robust and efficient method for the extraction of plant extracellular surface lipids as applied to the analysis of silks and seedling leaves of maize},
author = {Loneman, Derek M. and Peddicord, Layton and Al-Rashid, Amani and Nikolau, Basil J. and Lauter, Nick and Yandeau-Nelson, Marna D. and Andrade, Paula B.},
abstractNote = {Aerial plant organs possess a diverse array of extracellular surface lipids, including both non-polar and amphipathic constituents that collectively provide a primary line of defense against environmental stressors. Extracellular surface lipids on the stigmatic silks of maize are composed primarily of saturated and unsaturated linear hydrocarbons, as well as fatty acids, and aldehydes. To efficiently extract lipids of differing polarities from maize silks, five solvent systems (hexanes; hexanes:diethyl ether (95:5); hexanes:diethyl ether (90:10); chloroform:hexanes (1:1) and chloroform) were tested by immersing fresh silks in solvent for different extraction times. Surface lipid recovery and the relative composition of individual constituents were impacted to varying degrees depending on solvent choice and duration of extraction. Analyses were performed using both silks and leaves to demonstrate the utility of the solvent- and time-optimized protocol in comparison to extraction with the commonly used chloroform solvent. Overall, the preferred solvent system was identified as hexanes:diethyl ether (90:10), based on its effectiveness in extracting surface hydrocarbons and fatty acids as well as its reduced propensity to extract presumed internal fatty acids. Metabolite profiling of wildtype and glossy1 seedlings, which are impaired in surface lipid biosynthesis, demonstrated the ability of the preferred solvent to extract extracellular surface lipids rich in amphipathic compounds (aldehydes and alcohols). In addition to the expected deficiencies in dotriacontanal and dotriacontan-1-ol for gl1 seedlings, an unexpected increase in fatty acid recovery was observed in gl1 seedlings extracted in chloroform, suggesting that chloro-form extracts lipids from internal tissues of gl1 seedlings. This highlights the importance of extraction method when evaluating mutants that have altered cuticular lipid compositions. Lastly, metabolite profiling of silks from maize inbreds B73 and Mo17, exposed to different environments and harvested at different ages, revealed differences in hydrocarbon and fatty acid composition, demonstrating the dynamic nature of surface lipid accumulation on silks.},
doi = {10.1371/journal.pone.0180850},
journal = {PLoS ONE},
number = 7,
volume = 12,
place = {United States},
year = {2017},
month = {7}
}

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Works referenced in this record:

A Single-Step Method for Rapid Extraction of Total Lipids from Green Microalgae
journal, February 2014


An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data
journal, August 1999


Comparison of several methods for effective lipid extraction from microalgae
journal, January 2010


The Formation and Function of Plant Cuticles
journal, July 2013


A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959

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