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Title: Metabolic Reconstruction and Modeling Microbial Electrosynthesis

Abstract

Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. Here, we assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. The resulting molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.

Authors:
 [1];  [2]; ORCiD logo [3];  [4];  [3];  [3];  [5];  [6];  [2]
  1. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division; Univ. of Chicago, IL (United States). Dept. of Surgery
  2. Univ. of South Carolina, Columbia, SC (United States). Dept. of Environmental Health Sciences
  3. Argonne National Lab. (ANL), Argonne, IL (United States). Computing Environment and Life Sciences
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division
  5. Argonne National Lab. (ANL), Argonne, IL (United States). Biosciences Division; Univ. of Chicago, IL (United States). Dept. of Ecology and Evolution, Dept. of Surgery
  6. Medical Univ. of South Carolina, Charleston, SC (United States). Dept. of Microbiology and Immunology, Marine Biomedicine and Environmental Science Center
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1393156
Grant/Contract Number:  
AC02-06CH11357; AR0000089
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; applied microbiology; metagenomics

Citation Formats

Marshall, Christopher W., Ross, Daniel E., Handley, Kim M., Weisenhorn, Pamela B., Edirisinghe, Janaka N., Henry, Christopher S., Gilbert, Jack A., May, Harold D., and Norman, R. Sean. Metabolic Reconstruction and Modeling Microbial Electrosynthesis. United States: N. p., 2017. Web. doi:10.1038/s41598-017-08877-z.
Marshall, Christopher W., Ross, Daniel E., Handley, Kim M., Weisenhorn, Pamela B., Edirisinghe, Janaka N., Henry, Christopher S., Gilbert, Jack A., May, Harold D., & Norman, R. Sean. Metabolic Reconstruction and Modeling Microbial Electrosynthesis. United States. doi:10.1038/s41598-017-08877-z.
Marshall, Christopher W., Ross, Daniel E., Handley, Kim M., Weisenhorn, Pamela B., Edirisinghe, Janaka N., Henry, Christopher S., Gilbert, Jack A., May, Harold D., and Norman, R. Sean. Mon . "Metabolic Reconstruction and Modeling Microbial Electrosynthesis". United States. doi:10.1038/s41598-017-08877-z. https://www.osti.gov/servlets/purl/1393156.
@article{osti_1393156,
title = {Metabolic Reconstruction and Modeling Microbial Electrosynthesis},
author = {Marshall, Christopher W. and Ross, Daniel E. and Handley, Kim M. and Weisenhorn, Pamela B. and Edirisinghe, Janaka N. and Henry, Christopher S. and Gilbert, Jack A. and May, Harold D. and Norman, R. Sean},
abstractNote = {Microbial electrosynthesis is a renewable energy and chemical production platform that relies on microbial cells to capture electrons from a cathode and fix carbon. Yet despite the promise of this technology, the metabolic capacity of the microbes that inhabit the electrode surface and catalyze electron transfer in these systems remains largely unknown. Here, we assembled thirteen draft genomes from a microbial electrosynthesis system producing primarily acetate from carbon dioxide, and their transcriptional activity was mapped to genomes from cells on the electrode surface and in the supernatant. This allowed us to create a metabolic model of the predominant community members belonging to Acetobacterium, Sulfurospirillum, and Desulfovibrio. According to the model, the Acetobacterium was the primary carbon fixer, and a keystone member of the community. Transcripts of soluble hydrogenases and ferredoxins from Acetobacterium and hydrogenases, formate dehydrogenase, and cytochromes of Desulfovibrio were found in high abundance near the electrode surface. Cytochrome c oxidases of facultative members of the community were highly expressed in the supernatant despite completely sealed reactors and constant flushing with anaerobic gases. The resulting molecular discoveries and metabolic modeling now serve as a foundation for future examination and development of electrosynthetic microbial communities.},
doi = {10.1038/s41598-017-08877-z},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {8}
}

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    Works referencing / citing this record:

    Mo2C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO2 reduction
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    Continuous n-valerate formation from propionate and methanol in an anaerobic chain elongation open-culture bioreactor
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    • de Smit, Sanne M.; de Leeuw, Kasper D.; Buisman, Cees J. N.
    • Biotechnology for Biofuels, Vol. 12, Issue 1
    • DOI: 10.1186/s13068-019-1468-x

    Mo2C-induced hydrogen production enhances microbial electrosynthesis of acetate from CO2 reduction
    journal, April 2019


    Continuous n-valerate formation from propionate and methanol in an anaerobic chain elongation open-culture bioreactor
    journal, May 2019

    • de Smit, Sanne M.; de Leeuw, Kasper D.; Buisman, Cees J. N.
    • Biotechnology for Biofuels, Vol. 12, Issue 1
    • DOI: 10.1186/s13068-019-1468-x