Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development
Abstract
Aliphatic and aromatic lipids are both essential structural components of the plant cuticle, an important interface between the plant and environment. Although cross links between aromatic and aliphatic or other moieties are known to be associated with the formation of leaf cutin and root and seed suberin, the contribution of aromatic lipids to the biosynthesis of anther cuticles and pollen walls remains elusive. In this study, we characterized the rice (Oryza sativa) male sterile mutant, defective pollen wall 2 (dpw2), which showed an abnormal anther cuticle, a defective pollen wall, and complete male sterility. Compared with the wild type, dpw2 anthers have increased amounts of cutin and waxes and decreased levels of lipidic and phenolic compounds. DPW2 encodes a cytoplasmically localized BAHD acyltransferase. In vitro assays demonstrated that recombinant DPW2 specifically transfers hydroxycinnamic acid moieties, using v-hydroxy fatty acids as acyl acceptors and hydroxycinnamoyl-CoAs as acyl donors. Thus, The cytoplasmic hydroxycinnamoyl-CoA:v-hydroxy fatty acid transferase DPW2 plays a fundamental role in male reproduction via the biosynthesis of key components of the anther cuticle and pollen wall.
- Authors:
-
[1];
[1];
[4];
[2];
[5];
[6];
[7];
[1]
- Shanghai Jiao Tong Univ. (China). Joint International Research Lab. of Metabolic and Developmental Sciences
- Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Biological Systems and Engineering Division
- Kyung Hee Univ., Yongin (Korea). Crop Biotech Inst.
- Univ. of Bonn (Germany). Inst. of Cellular and Molecular Botany
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Biological Systems and Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
- Michigan State Univ., East Lansing, MI (United States). Dept. of Energy Plant Research Lab.
- Shanghai Jiao Tong Univ. (China). Joint International Research Lab. of Metabolic and Developmental Sciences; Univ. of Adelaide, SA (Australia). School of Agriculture, Food and Wine
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1393111
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda); Journal Volume: 173; Journal Issue: 1; Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Xu, Dawei, Shi, Jianxin, Rautengarten, Carsten, Yang, Li, Qian, Xiaoling, Uzair, Muhammad, Zhu, Lu, Luo, Qian, An, Gynheung, Waßmann, Fritz, Schreiber, Lukas, Heazlewood, Joshua L., Scheller, Henrik Vibe, Hu, Jianping, Zhang, Dabing, and Liang, Wanqi. Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development. United States: N. p., 2016.
Web. doi:10.1104/pp.16.00095.
Xu, Dawei, Shi, Jianxin, Rautengarten, Carsten, Yang, Li, Qian, Xiaoling, Uzair, Muhammad, Zhu, Lu, Luo, Qian, An, Gynheung, Waßmann, Fritz, Schreiber, Lukas, Heazlewood, Joshua L., Scheller, Henrik Vibe, Hu, Jianping, Zhang, Dabing, & Liang, Wanqi. Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development. United States. https://doi.org/10.1104/pp.16.00095
Xu, Dawei, Shi, Jianxin, Rautengarten, Carsten, Yang, Li, Qian, Xiaoling, Uzair, Muhammad, Zhu, Lu, Luo, Qian, An, Gynheung, Waßmann, Fritz, Schreiber, Lukas, Heazlewood, Joshua L., Scheller, Henrik Vibe, Hu, Jianping, Zhang, Dabing, and Liang, Wanqi. Tue .
"Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development". United States. https://doi.org/10.1104/pp.16.00095. https://www.osti.gov/servlets/purl/1393111.
@article{osti_1393111,
title = {Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development},
author = {Xu, Dawei and Shi, Jianxin and Rautengarten, Carsten and Yang, Li and Qian, Xiaoling and Uzair, Muhammad and Zhu, Lu and Luo, Qian and An, Gynheung and Waßmann, Fritz and Schreiber, Lukas and Heazlewood, Joshua L. and Scheller, Henrik Vibe and Hu, Jianping and Zhang, Dabing and Liang, Wanqi},
abstractNote = {Aliphatic and aromatic lipids are both essential structural components of the plant cuticle, an important interface between the plant and environment. Although cross links between aromatic and aliphatic or other moieties are known to be associated with the formation of leaf cutin and root and seed suberin, the contribution of aromatic lipids to the biosynthesis of anther cuticles and pollen walls remains elusive. In this study, we characterized the rice (Oryza sativa) male sterile mutant, defective pollen wall 2 (dpw2), which showed an abnormal anther cuticle, a defective pollen wall, and complete male sterility. Compared with the wild type, dpw2 anthers have increased amounts of cutin and waxes and decreased levels of lipidic and phenolic compounds. DPW2 encodes a cytoplasmically localized BAHD acyltransferase. In vitro assays demonstrated that recombinant DPW2 specifically transfers hydroxycinnamic acid moieties, using v-hydroxy fatty acids as acyl acceptors and hydroxycinnamoyl-CoAs as acyl donors. Thus, The cytoplasmic hydroxycinnamoyl-CoA:v-hydroxy fatty acid transferase DPW2 plays a fundamental role in male reproduction via the biosynthesis of key components of the anther cuticle and pollen wall.},
doi = {10.1104/pp.16.00095},
journal = {Plant Physiology (Bethesda)},
number = 1,
volume = 173,
place = {United States},
year = {Tue May 31 00:00:00 EDT 2016},
month = {Tue May 31 00:00:00 EDT 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 75 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referencing / citing this record:
A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley
journal, November 2017
- Li, Chao; Chen, Guoxiong; Mishina, Kohei
- Plant Direct, Vol. 1, Issue 5
OsPKS2 is required for rice male fertility by participating in pollen wall formation
journal, February 2018
- Zou, Ting; Liu, Mingxing; Xiao, Qiao
- Plant Cell Reports, Vol. 37, Issue 5
Untargeted metabolomic analysis of tomato pollen development and heat stress response
journal, May 2017
- Paupière, Marine J.; Müller, Florian; Li, Hanjing
- Plant Reproduction, Vol. 30, Issue 2
Knockout of OsACOS12 caused male sterility in rice
journal, September 2017
- Zou, Ting; He, Zhiyuan; Qu, Lingyun
- Molecular Breeding, Vol. 37, Issue 10
OsMS1 functions as a transcriptional activator to regulate programmed tapetum development and pollen exine formation in rice
journal, January 2019
- Yang, Zhengfu; Liu, Ling; Sun, Lianping
- Plant Molecular Biology, Vol. 99, Issue 1-2
Pollen development and function under heat stress: from effects to responses
journal, March 2019
- Raja, Mondam Muni; Vijayalakshmi, Gujjula; Naik, Mude Lakshmipathi
- Acta Physiologiae Plantarum, Vol. 41, Issue 4
The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice: OsPKS2 is essential for pollen exine patterning in rice
journal, September 2017
- Zhu, Xiaolei; Yu, Jing; Shi, Jianxin
- Journal of Integrative Plant Biology, Vol. 59, Issue 9
ABNORMAL POLLEN VACUOLATION1 (APV1) is required for male fertility by contributing to anther cuticle and pollen exine formation in maize
journal, March 2017
- Somaratne, Yamuna; Tian, Youhui; Zhang, Hua
- The Plant Journal, Vol. 90, Issue 1
RiceAntherNet: a gene co‐expression network for identifying anther and pollen development genes
journal, November 2017
- Lin, Hong; Yu, Jing; Pearce, Simon P.
- The Plant Journal, Vol. 92, Issue 6
The ATP-binding cassette (ABC) transporter OsABCG3 is essential for pollen development in rice
journal, October 2018
- Chang, Zhenyi; Jin, Mingna; Yan, Wei
- Rice, Vol. 11, Issue 1
Salicylic acid reverses pollen abortion of rice caused by heat stress
journal, October 2018
- Feng, Baohua; Zhang, Caixia; Chen, Tingting
- BMC Plant Biology, Vol. 18, Issue 1
Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
journal, March 2019
- Xu, Dawei; Qu, Shuying; Tucker, Matthew R.
- BMC Plant Biology, Vol. 19, Issue 1
Comprehensive analysis of Ogura cytoplasmic male sterility-related genes in turnip (Brassica rapa ssp. rapifera) using RNA sequencing analysis and bioinformatics
journal, June 2019
- Lin, Sue; Miao, Yingjing; Su, Shiwen
- PLOS ONE, Vol. 14, Issue 6
A GDSL-motif esterase/acyltransferase/lipase is responsible for leaf water retention in barley
journal, November 2017
- Li, Chao; Chen, Guoxiong; Mishina, Kohei
- Plant Direct, Vol. 1, Issue 5
Untargeted metabolomic analysis of tomato pollen development and heat stress response
journal, May 2017
- Paupière, Marine J.; Müller, Florian; Li, Hanjing
- Plant Reproduction, Vol. 30, Issue 2
The ATP-binding cassette (ABC) transporter OsABCG3 is essential for pollen development in rice
journal, October 2018
- Chang, Zhenyi; Jin, Mingna; Yan, Wei
- Rice, Vol. 11, Issue 1
Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
journal, March 2019
- Xu, Dawei; Qu, Shuying; Tucker, Matthew R.
- BMC Plant Biology, Vol. 19, Issue 1
Comprehensive analysis of Ogura cytoplasmic male sterility-related genes in turnip (Brassica rapa ssp. rapifera) using RNA sequencing analysis and bioinformatics
journal, June 2019
- Lin, Sue; Miao, Yingjing; Su, Shiwen
- PLOS ONE, Vol. 14, Issue 6
OsGPAT3 Plays a Critical Role in Anther Wall Programmed Cell Death and Pollen Development in Rice
journal, December 2018
- Sun, Lianping; Xiang, Xiaojiao; Yang, Zhengfu
- International Journal of Molecular Sciences, Vol. 19, Issue 12