Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

Beller, Harry R.; Zhou, Peng; Jewell, Talia N. M.; Goh, Ee-Been; Keasling, Jay D.

doi:10.1016/j.meteno.2016.07.001

Title: Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources

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Abstract

Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H ₂ S, while fixing CO ₂ are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering of the chemolithoautotrophic bacterium Thiobacillus denitrificans to produce up to 52-fold more fatty acids than the wild-type strain when grown with thiosulfate and CO ₂ . A modified thioesterase gene from E. coli ('tesA) was integrated into the T. denitrificans chromosome under the control of P _kan or one of two native T. denitrificans promoters. The relative strength of the two native promoters as assessed by fatty acid production in engineered strains was very similar to that assessed by expression of the cognate genes in the wild-type strain. This proof-of-principle study suggests that engineering sulfide-oxidizing chemolithoautotrophic bacteria to overproduce fatty acid-derived products merits consideration as a technology that could simultaneously produce renewable fuels/chemicals as well as cost-effectively remediate sulfide-contaminated wastewater.

Authors:: Beller, Harry R.; Zhou, Peng; Jewell, Talia N. M.; Goh, Ee-Been; Keasling, Jay D.

Publication Date:: Thu Dec 01 00:00:00 EST 2016

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1394664

Alternate Identifier(s):: OSTI ID: 1393092

Grant/Contract Number:: AC02–05CH11231; AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Metabolic Engineering Communications

Additional Journal Information:: Journal Name: Metabolic Engineering Communications Journal Volume: 3 Journal Issue: C; Journal ID: ISSN 2214-0301

Publisher:: Elsevier

Country of Publication:: Netherlands

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; chemolithoautotrophic; sulfide; fatty acids; tesA; thiobacillus denitrificans

Citation Formats


                    Beller, Harry R., Zhou, Peng, Jewell, Talia N. M., Goh, Ee-Been, and Keasling, Jay D. Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources.  Netherlands: N. p., 2016. 
Web.  doi:10.1016/j.meteno.2016.07.001.

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                    Beller, Harry R., Zhou, Peng, Jewell, Talia N. M., Goh, Ee-Been, & Keasling, Jay D. Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources.  Netherlands.  https://doi.org/10.1016/j.meteno.2016.07.001

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                    Beller, Harry R., Zhou, Peng, Jewell, Talia N. M., Goh, Ee-Been, and Keasling, Jay D. Thu .  
"Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources".  Netherlands.  https://doi.org/10.1016/j.meteno.2016.07.001.

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@article{osti_1394664,

  title        = {Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources},

  author       = {Beller, Harry R. and Zhou, Peng and Jewell, Talia N. M. and Goh, Ee-Been and Keasling, Jay D.},

  abstractNote = {Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H 2 S, while fixing CO 2 are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering of the chemolithoautotrophic bacterium Thiobacillus denitrificans to produce up to 52-fold more fatty acids than the wild-type strain when grown with thiosulfate and CO 2 . A modified thioesterase gene from E. coli ('tesA) was integrated into the T. denitrificans chromosome under the control of P kan or one of two native T. denitrificans promoters. The relative strength of the two native promoters as assessed by fatty acid production in engineered strains was very similar to that assessed by expression of the cognate genes in the wild-type strain. This proof-of-principle study suggests that engineering sulfide-oxidizing chemolithoautotrophic bacteria to overproduce fatty acid-derived products merits consideration as a technology that could simultaneously produce renewable fuels/chemicals as well as cost-effectively remediate sulfide-contaminated wastewater.},

  doi          = {10.1016/j.meteno.2016.07.001},

  journal      = {Metabolic Engineering Communications},

  number       = C,

  volume       = 3,

  place        = {Netherlands},

  year         = {Thu Dec 01 00:00:00 EST 2016},

  month        = {Thu Dec 01 00:00:00 EST 2016}

}

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