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Title: BIG LEAF is a regulator of organ size and adventitious root formation in poplar

Abstract

Here we report the discovery through activation tagging and subsequent characterization of the BIG LEAF (BL) gene from poplar. In poplar, BL regulates leaf size via positively affecting cell proliferation. Up and downregulation of the gene led to increased and decreased leaf size, respectively, and these phenotypes corresponded to increased and decreased cell numbers. BL function encompasses the early stages of leaf development as native BL expression was specific to the shoot apical meristem and leaf primordia and was absent from the later stages of leaf development and other organs. Consistently, BL downregulation reduced leaf size at the earliest stages of leaf development. Ectopic expression in mature leaves resulted in continued growth most probably via sustained cell proliferation and thus the increased leaf size. In contrast to the positive effect on leaf growth, ectopic BL expression in stems interfered with and significantly reduced stem thickening, suggesting that BL is a highly specific activator of growth. Additionally, stem cuttings from BL overexpressing plants developed roots, whereas the wild type was difficult to root, demonstrating that BL is a positive regulator of adventitious rooting. Large transcriptomic changes in plants that overexpressed BL indicated that BL may have a broad integrative role, encompassingmore » many genes linked to organ growth. Here, we conclude that BL plays a fundamental role in control of leaf size and thus may be a useful tool for modifying plant biomass productivity and adventitious rooting.« less

Authors:
 [1];  [2];  [1];  [3];  [2];  [4];  [5]
  1. Michigan Technological Univ., Houghton, MI (United States). School of Forest Resources and Environmental Science; Eastern Illinois Univ., Charleston, IL (United States). Dept. of Biological Sciences
  2. Oregon State Univ., Corvallis, OR (United States). Dept. of Forest Ecosystems and Society
  3. Purdue Univ., West Lafayette, IN (United States). Dept. of Forestry and Natural Resources
  4. Michigan Technological Univ., Houghton, MI (United States). School of Forest Resources and Environmental Science
  5. Universidad Miguel Hernandez de Elche (Spain)
Publication Date:
Research Org.:
Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States); Oregon State Univ., Corvallis, OR (United States); Michigan Technological Univ., Houghton, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1392995
Grant/Contract Number:  
FG02-06ER64185; FG02-05ER64113; SC0008462
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Yordanov, Yordan S., Ma, Cathleen, Yordanova, Elena, Meilan, Richard, Strauss, Steven H., Busov, Victor B., and Candela, Hector. BIG LEAF is a regulator of organ size and adventitious root formation in poplar. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0180527.
Yordanov, Yordan S., Ma, Cathleen, Yordanova, Elena, Meilan, Richard, Strauss, Steven H., Busov, Victor B., & Candela, Hector. BIG LEAF is a regulator of organ size and adventitious root formation in poplar. United States. https://doi.org/10.1371/journal.pone.0180527
Yordanov, Yordan S., Ma, Cathleen, Yordanova, Elena, Meilan, Richard, Strauss, Steven H., Busov, Victor B., and Candela, Hector. Fri . "BIG LEAF is a regulator of organ size and adventitious root formation in poplar". United States. https://doi.org/10.1371/journal.pone.0180527. https://www.osti.gov/servlets/purl/1392995.
@article{osti_1392995,
title = {BIG LEAF is a regulator of organ size and adventitious root formation in poplar},
author = {Yordanov, Yordan S. and Ma, Cathleen and Yordanova, Elena and Meilan, Richard and Strauss, Steven H. and Busov, Victor B. and Candela, Hector},
abstractNote = {Here we report the discovery through activation tagging and subsequent characterization of the BIG LEAF (BL) gene from poplar. In poplar, BL regulates leaf size via positively affecting cell proliferation. Up and downregulation of the gene led to increased and decreased leaf size, respectively, and these phenotypes corresponded to increased and decreased cell numbers. BL function encompasses the early stages of leaf development as native BL expression was specific to the shoot apical meristem and leaf primordia and was absent from the later stages of leaf development and other organs. Consistently, BL downregulation reduced leaf size at the earliest stages of leaf development. Ectopic expression in mature leaves resulted in continued growth most probably via sustained cell proliferation and thus the increased leaf size. In contrast to the positive effect on leaf growth, ectopic BL expression in stems interfered with and significantly reduced stem thickening, suggesting that BL is a highly specific activator of growth. Additionally, stem cuttings from BL overexpressing plants developed roots, whereas the wild type was difficult to root, demonstrating that BL is a positive regulator of adventitious rooting. Large transcriptomic changes in plants that overexpressed BL indicated that BL may have a broad integrative role, encompassing many genes linked to organ growth. Here, we conclude that BL plays a fundamental role in control of leaf size and thus may be a useful tool for modifying plant biomass productivity and adventitious rooting.},
doi = {10.1371/journal.pone.0180527},
journal = {PLoS ONE},
number = 7,
volume = 12,
place = {United States},
year = {Fri Jul 07 00:00:00 EDT 2017},
month = {Fri Jul 07 00:00:00 EDT 2017}
}

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EARLY BUD-BREAK 1 ( EBB1 ) is a regulator of release from seasonal dormancy in poplar trees
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journal, May 1993

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A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta
journal, September 2003

  • Curtis, Mark D.; Grossniklaus, Ueli
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The orf13 T-DNA Gene of Agrobacterium rhizogenes Confers Meristematic Competence to Differentiated Cells
journal, July 2004

  • Stieger, Pia A.; Meyer, Alain D.; Kathmann, Petra
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The AINTEGUMENTA LIKE1 Homeotic Transcription Factor PtAIL1 Controls the Formation of Adventitious Root Primordia in Poplar
journal, October 2012

  • Rigal, Adeline; Yordanov, Yordan S.; Perrone, Irene
  • Plant Physiology, Vol. 160, Issue 4
  • DOI: 10.1104/pp.112.204453

Members of the LATERAL ORGAN BOUNDARIES DOMAIN Transcription Factor Family Are Involved in the Regulation of Secondary Growth in Populus
journal, November 2010

  • Yordanov, Yordan S.; Regan, Sharon; Busov, Victor
  • The Plant Cell, Vol. 22, Issue 11
  • DOI: 10.1105/tpc.110.078634

A Repressor Protein Complex Regulates Leaf Growth in Arabidopsis
journal, July 2015

  • Gonzalez, Nathalie; Pauwels, Laurens; Baekelandt, Alexandra
  • The Plant Cell, Vol. 27, Issue 8
  • DOI: 10.1105/tpc.15.00006

Detection of protein-protein interactions in plants using the transrepressive activity of the EAR motif repression domain
journal, February 2010


Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus
journal, October 2006


Genetic control of plant organ growth
journal, April 2011


Nitrogen deprivation promotes Populus root growth through global transcriptome reprogramming and activation of hierarchical genetic networks
journal, June 2013

  • Wei, Hairong; Yordanov, Yordan S.; Georgieva, Tatyana
  • New Phytologist, Vol. 200, Issue 2
  • DOI: 10.1111/nph.12375

Adventitious root formation in tree species: involvement of transcription factors
journal, May 2014

  • Legué, Valérie; Rigal, Adeline; Bhalerao, Rishikesh P.
  • Physiologia Plantarum, Vol. 151, Issue 2
  • DOI: 10.1111/ppl.12197

Flower Development
journal, January 2010

  • Alvarez-Buylla, Elena R.; Benítez, Mariana; Corvera-Poiré, Adriana
  • The Arabidopsis Book, Vol. 8
  • DOI: 10.1199/tab.0127

Behavior of Leaf Meristems and Their Modification
journal, December 2015


Works referencing / citing this record: