DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study

Abstract

Abstract This work aims to explore the concentration‐dependence of SPIO‐Au core–shell nanoscale particles (NPs) (17.3 ± 1.2 nm in diameter) on biocompatibility and osteogenic differentiation of preosteoblast MC3T3‐E1 cells. The stability of NPs was first investigated by UV–vis absorption spectra and zeta potential measurement. Then concentration effects of NPs (1–80 μg/mL) were evaluated on viability, morphology, proliferation, cellular uptake, and alkaline phosphate (ALP) activity levels. Results have shown strong stability and no acute toxicity (viability > 93%) or morphological difference at all concentration levels of NPs. The proliferation results indicated that the concentration of NPs below 40 μg/mL does not affect the cell proliferation for 7 days of incubation. Transmission electron microscopy images revealed the successful internalization of NPs into MC3T3‐E1 cells and the dose‐dependent accumulation of NPs inside the cytoplasm. The ALP level of MC3T3‐E1 cells was improved by 49% (of control) after treated with NPs at 10 μg/mL for 10 days, indicating their positive effect on early osteogenic differentiation. This study confirmed the excellent biocompatibility of SPIO‐Au NPs and their great potential for promoting osteogenic differentiation and promised the future application for these NPs in bone engineering including drug delivery, cell labeling, and activity tracking within scaffolds. © 2017 Wiley Periodicals, Inc.more » J Biomed Mater Res Part A: 105A: 3350–3359, 2017.« less

Authors:
 [1]; ORCiD logo [2];  [3]
  1. Heavy Engineering 133, Department of Mechanical Engineering State University of New York at Stony Brook Stony Brook New York 11794‐2300
  2. LE 153, Department of Mechanical Engineering State University of New York at Stony Brook Stony Brook New York 11794‐2300
  3. Department of Biomedical Engineering, 215 Bioengineering Building State University of New York at Stony Brook Stony Brook New York 11794‐5281
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1392697
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Biomedical Materials Research. Part A
Additional Journal Information:
Journal Name: Journal of Biomedical Materials Research. Part A Journal Volume: 105 Journal Issue: 12; Journal ID: ISSN 1549-3296
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States
Language:
English

Citation Formats

Yuan, Muzhaozi, Wang, Ya, and Qin, Yi‐Xian. SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study. United States: N. p., 2017. Web. doi:10.1002/jbm.a.36200.
Yuan, Muzhaozi, Wang, Ya, & Qin, Yi‐Xian. SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study. United States. https://doi.org/10.1002/jbm.a.36200
Yuan, Muzhaozi, Wang, Ya, and Qin, Yi‐Xian. Tue . "SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study". United States. https://doi.org/10.1002/jbm.a.36200.
@article{osti_1392697,
title = {SPIO‐Au core–shell nanoparticles for promoting osteogenic differentiation of MC3T3‐E1 cells: Concentration‐dependence study},
author = {Yuan, Muzhaozi and Wang, Ya and Qin, Yi‐Xian},
abstractNote = {Abstract This work aims to explore the concentration‐dependence of SPIO‐Au core–shell nanoscale particles (NPs) (17.3 ± 1.2 nm in diameter) on biocompatibility and osteogenic differentiation of preosteoblast MC3T3‐E1 cells. The stability of NPs was first investigated by UV–vis absorption spectra and zeta potential measurement. Then concentration effects of NPs (1–80 μg/mL) were evaluated on viability, morphology, proliferation, cellular uptake, and alkaline phosphate (ALP) activity levels. Results have shown strong stability and no acute toxicity (viability > 93%) or morphological difference at all concentration levels of NPs. The proliferation results indicated that the concentration of NPs below 40 μg/mL does not affect the cell proliferation for 7 days of incubation. Transmission electron microscopy images revealed the successful internalization of NPs into MC3T3‐E1 cells and the dose‐dependent accumulation of NPs inside the cytoplasm. The ALP level of MC3T3‐E1 cells was improved by 49% (of control) after treated with NPs at 10 μg/mL for 10 days, indicating their positive effect on early osteogenic differentiation. This study confirmed the excellent biocompatibility of SPIO‐Au NPs and their great potential for promoting osteogenic differentiation and promised the future application for these NPs in bone engineering including drug delivery, cell labeling, and activity tracking within scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3350–3359, 2017.},
doi = {10.1002/jbm.a.36200},
journal = {Journal of Biomedical Materials Research. Part A},
number = 12,
volume = 105,
place = {United States},
year = {Tue Sep 19 00:00:00 EDT 2017},
month = {Tue Sep 19 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/jbm.a.36200

Citation Metrics:
Cited by: 22 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

A Gold Nanoparticle Pentapeptide: Gene Fusion To Induce Therapeutic Gene Expression in Mesenchymal Stem Cells
journal, October 2014

  • Muroski, Megan E.; Morgan, Thomas J.; Levenson, Cathy W.
  • Journal of the American Chemical Society, Vol. 136, Issue 42
  • DOI: 10.1021/ja505190q

A dual gold nanoparticle system for mesenchymal stem cell tracking
journal, January 2014

  • Ricles, L. M.; Nam, S. Y.; Treviño, E. A.
  • J. Mater. Chem. B, Vol. 2, Issue 46
  • DOI: 10.1039/C4TB00975D

Biocompatibility of silver nanoparticles and silver ions in primary human mesenchymal stem cells and osteoblasts
journal, January 2014


Hybrid magnetic scaffolds of gelatin–siloxane incorporated with magnetite nanoparticles effective for bone tissue engineering
journal, January 2014

  • Dashnyam, Khandmaa; Perez, Roman A.; Singh, Rajendra K.
  • RSC Adv., Vol. 4, Issue 77
  • DOI: 10.1039/C4RA06621A

Can Bone Morphogenetic Protein Binding Peptide Increase Efficiency of Bone Formation?
journal, January 2010


Determining the Size and Shape Dependence of Gold Nanoparticle Uptake into Mammalian Cells
journal, April 2006

  • Chithrani, B. Devika; Ghazani, Arezou A.; Chan, Warren C. W.
  • Nano Letters, Vol. 6, Issue 4
  • DOI: 10.1021/nl052396o

Synthesis of Fe Oxide Core/Au Shell Nanoparticles by Iterative Hydroxylamine Seeding
journal, April 2004

  • Lyon, Jennifer L.; Fleming, David A.; Stone, Matthew B.
  • Nano Letters, Vol. 4, Issue 4, p. 719-723
  • DOI: 10.1021/nl035253f

Gold Nanoparticles Promote Osteogenic Differentiation of Mesenchymal Stem Cells through p38 MAPK Pathway
journal, October 2010

  • Yi, Changqing; Liu, Dandan; Fong, Chi-Chun
  • ACS Nano, Vol. 4, Issue 11
  • DOI: 10.1021/nn101373r

Gold Nanoparticles in Chemical and Biological Sensing
journal, February 2012

  • Saha, Krishnendu; Agasti, Sarit S.; Kim, Chaekyu
  • Chemical Reviews, Vol. 112, Issue 5
  • DOI: 10.1021/cr2001178

Micrometer-sized iron oxide particle labeling of mesenchymal stem cells for magnetic resonance imaging-based monitoring of cartilage tissue engineering
journal, January 2011

  • Saldanha, Karl J.; Doan, Ryan P.; Ainslie, Kristy M.
  • Magnetic Resonance Imaging, Vol. 29, Issue 1
  • DOI: 10.1016/j.mri.2010.07.015

The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems
journal, August 2012


Effect of Gold Nanoparticle Aggregation on Cell Uptake and Toxicity
journal, June 2011

  • Albanese, Alexandre; Chan, Warren C. W.
  • ACS Nano, Vol. 5, Issue 7
  • DOI: 10.1021/nn2007496

Enhanced bone regeneration with a gold nanoparticle–hydrogel complex
journal, January 2014

  • Heo, Dong Nyoung; Ko, Wan-Kyu; Bae, Min Soo
  • J. Mater. Chem. B, Vol. 2, Issue 11
  • DOI: 10.1039/C3TB21246G

Nanostructured scaffolds for bone tissue engineering
journal, February 2013

  • Li, Xiaoming; Wang, Lu; Fan, Yubo
  • Journal of Biomedical Materials Research Part A, Vol. 101A, Issue 8
  • DOI: 10.1002/jbm.a.34539

Imaging Strategies for Tissue Engineering Applications
journal, February 2015

  • Nam, Seung Yun; Ricles, Laura M.; Suggs, Laura J.
  • Tissue Engineering Part B: Reviews, Vol. 21, Issue 1
  • DOI: 10.1089/ten.teb.2014.0180

Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological Applications
journal, June 2008

  • Laurent, Sophie; Forge, Delphine; Port, Marc
  • Chemical Reviews, Vol. 108, Issue 6, p. 2064-2110
  • DOI: 10.1021/cr068445e

Magnetic Core/Shell Fe 3 O 4 /Au and Fe 3 O 4 /Au/Ag Nanoparticles with Tunable Plasmonic Properties
journal, July 2007

  • Xu, Zhichuan; Hou, Yanglong; Sun, Shouheng
  • Journal of the American Chemical Society, Vol. 129, Issue 28
  • DOI: 10.1021/ja073057v

Superparamagnetic iron oxide nanoparticles (SPIONs): Development, surface modification and applications in chemotherapy
journal, January 2011

  • Mahmoudi, Morteza; Sant, Shilpa; Wang, Ben
  • Advanced Drug Delivery Reviews, Vol. 63, Issue 1-2, p. 24-46
  • DOI: 10.1016/j.addr.2010.05.006

Nanoparticles and their potential for application in bone
journal, August 2012


Biocompatibility of Tungsten Disulfide Inorganic Nanotubes and Fullerene-Like Nanoparticles with Salivary Gland Cells
journal, March 2015

  • Goldman, Elisheva B.; Zak, Alla; Tenne, Reshef
  • Tissue Engineering Part A, Vol. 21, Issue 5-6
  • DOI: 10.1089/ten.tea.2014.0163

Superparamagnetic Iron Oxide Nanoparticles (SPIONs): Synthesis and Surface Modification Techniques for use with MRI and Other Biomedical Applications
journal, January 2013


Magnetic Resonance Functional Nano-Hydroxyapatite Incorporated Poly(Caprolactone) Composite Scaffolds for In Situ Monitoring of Bone Tissue Regeneration by MRI
journal, October 2014

  • Ganesh, Nitya; Ashokan, Anusha; Rajeshkannan, Ramiah
  • Tissue Engineering Part A, Vol. 20, Issue 19-20
  • DOI: 10.1089/ten.tea.2014.0161

Designed synthesis of uniformly sized iron oxide nanoparticles for efficient magnetic resonance imaging contrast agents
journal, January 2012


Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking
journal, January 2013


Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION)
journal, January 2010

  • Singh, Neenu; Jenkins, Gareth J. S.; Asadi, Romisa
  • Nano Reviews, Vol. 1, Issue 1
  • DOI: 10.3402/nano.v1i0.5358

In Vitro and In Vivo Chondrogenesis of Rabbit Bone Marrow–Derived Stromal Cells in Fibrin Matrix Mixed with Growth Factor Loaded in Nanoparticles
journal, August 2009


Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage
journal, December 2009

  • Ramaswamy, Sharan; Greco, Jane B.; Uluer, Mehmet C.
  • Tissue Engineering Part A, Vol. 15, Issue 12
  • DOI: 10.1089/ten.tea.2008.0677

Assessing the In Vitro and In Vivo Toxicity of Superparamagnetic Iron Oxide Nanoparticles
journal, December 2011

  • Mahmoudi, Morteza; Hofmann, Heinrich; Rothen-Rutishauser, Barbara
  • Chemical Reviews, Vol. 112, Issue 4
  • DOI: 10.1021/cr2002596

Nanomaterials promise better bone repair
journal, October 2016


Multifaceted applications of nanomaterials in cell engineering and therapy
journal, September 2013


Nanomaterial scaffolds for stem cell proliferation and differentiation in tissue engineering
journal, September 2013


Gold Nanoparticles Are Taken Up by Human Cells but Do Not Cause Acute Cytotoxicity
journal, March 2005

  • Connor, Ellen E.; Mwamuka, Judith; Gole, Anand
  • Small, Vol. 1, Issue 3, p. 325-327
  • DOI: 10.1002/smll.200400093

Optimization of the composition of bimetallic core/shell Fe2O3/Au nanoparticles for MRI/CT dual-mode imaging
journal, October 2013


RGD Peptide-Modified Dendrimer-Entrapped Gold Nanoparticles Enable Highly Efficient and Specific Gene Delivery to Stem Cells
journal, February 2015

  • Kong, Lingdan; Alves, Carla S.; Hou, Wenxiu
  • ACS Applied Materials & Interfaces, Vol. 7, Issue 8
  • DOI: 10.1021/am508760w

The golden age: gold nanoparticles for biomedicine
journal, January 2012

  • Dreaden, Erik C.; Alkilany, Alaaldin M.; Huang, Xiaohua
  • Chem. Soc. Rev., Vol. 41, Issue 7
  • DOI: 10.1039/C1CS15237H