skip to main content

DOE PAGESDOE PAGES

Title: Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome

Understanding microbial partnerships with the medicinally and economically important crop Cannabis has the potential to affect agricultural practice by improving plant fitness and production yield. Furthermore, Cannabis presents an interesting model to explore plant-microbiome interactions as it produces numerous secondary metabolic compounds. Here we present the first description of the endorhiza-, rhizosphere-, and bulk soil-associated microbiome of five distinct Cannabis cultivars. Bacterial communities of the endorhiza showed significant cultivar-specificity. When controlling cultivar and soil type the microbial community structure was significantly different between plant cultivars, soil types, and between the endorhiza, rhizosphere and soil. In conclusion, the influence of soil type, plant cultivar and sample type differentiation on the microbial community structure provides support for a previously published two-tier selection model, whereby community composition across sample types is determined mainly by soil type, while community structure within endorhiza samples is determined mainly by host cultivar.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [2] ;  [5] ;  [6] ;  [2] ;  [7]
  1. Dept. of Science and Education, Chicago, IL (United States); Univ. of Chicago, Chicago, IL (United States)
  2. Argonne National Lab. (ANL), Lemont, IL (United States); Univ. of Chicago, Chicago, IL (United States)
  3. Argonne National Lab. (ANL), Lemont, IL (United States); Basque Country Government, Bilbao (Spain)
  4. Dept. of Science and Education, Chicago, IL (United States)
  5. Cannavest, San Diego, CA (United States)
  6. MO BIO Labs, Carlsbad, CA (United States)
  7. Graz Univ. of Technology (TU Graz) (Austria)
Publication Date:
Grant/Contract Number:
AC02-06CH11357
Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 9; Journal Issue: 6; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
Argonne National Laboratory; USDOE; U.S. Department of Education; USEPA
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Cannabis Microbiome; Rhizosphere; Community structure; Microbiome; Cannabinoids; Cannabis sativa; Plant communities; Medical plants
OSTI Identifier:
1392596

Winston, Max E., Hampton-Marcell, Jarrad, Zarraonaindia, Iratxe, Owens, Sarah M., Moreau, Corrie S., Gilbert, Jack A., Hartsel, Josh, Kennedy, Suzanne J., Gibbons, Sean M., and Berg, Gabriele. Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome. United States: N. p., Web. doi:10.1371/journal.pone.0099641.
Winston, Max E., Hampton-Marcell, Jarrad, Zarraonaindia, Iratxe, Owens, Sarah M., Moreau, Corrie S., Gilbert, Jack A., Hartsel, Josh, Kennedy, Suzanne J., Gibbons, Sean M., & Berg, Gabriele. Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome. United States. doi:10.1371/journal.pone.0099641.
Winston, Max E., Hampton-Marcell, Jarrad, Zarraonaindia, Iratxe, Owens, Sarah M., Moreau, Corrie S., Gilbert, Jack A., Hartsel, Josh, Kennedy, Suzanne J., Gibbons, Sean M., and Berg, Gabriele. 2014. "Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome". United States. doi:10.1371/journal.pone.0099641. https://www.osti.gov/servlets/purl/1392596.
@article{osti_1392596,
title = {Understanding Cultivar-Specificity and Soil Determinants of the Cannabis Microbiome},
author = {Winston, Max E. and Hampton-Marcell, Jarrad and Zarraonaindia, Iratxe and Owens, Sarah M. and Moreau, Corrie S. and Gilbert, Jack A. and Hartsel, Josh and Kennedy, Suzanne J. and Gibbons, Sean M. and Berg, Gabriele},
abstractNote = {Understanding microbial partnerships with the medicinally and economically important crop Cannabis has the potential to affect agricultural practice by improving plant fitness and production yield. Furthermore, Cannabis presents an interesting model to explore plant-microbiome interactions as it produces numerous secondary metabolic compounds. Here we present the first description of the endorhiza-, rhizosphere-, and bulk soil-associated microbiome of five distinct Cannabis cultivars. Bacterial communities of the endorhiza showed significant cultivar-specificity. When controlling cultivar and soil type the microbial community structure was significantly different between plant cultivars, soil types, and between the endorhiza, rhizosphere and soil. In conclusion, the influence of soil type, plant cultivar and sample type differentiation on the microbial community structure provides support for a previously published two-tier selection model, whereby community composition across sample types is determined mainly by soil type, while community structure within endorhiza samples is determined mainly by host cultivar.},
doi = {10.1371/journal.pone.0099641},
journal = {PLoS ONE},
number = 6,
volume = 9,
place = {United States},
year = {2014},
month = {6}
}