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Title: Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Here, in this contribution we use nonconventional methods that help to increase the success rate of a protein crystal growth, and consequently of structural projects using X-ray diffraction techniques. In order to achieve this purpose, this contribution presents new approaches involving more sophisticated techniques of protein crystallization, not just for growing protein crystals of different sizes by using electric fields, but also for controlling crystal size and orientation. Also, this latter was possible through the use of magnetic fields that allow to obtain protein crystals suitable for both high-resolution X-ray and neutron diffraction crystallography where big crystals are required. This contribution discusses some pros, cons and realities of the role of electromagnetic fields in protein crystallization research, and their effect on protein crystal contacts. Additionally, we discuss the importance of room and low temperatures during data collection. Finally, we also discuss the effect of applying a rather strong magnetic field of 16.5 T, for shorts and long periods of time, on protein crystal growth, and on the 3D structure of two model proteins.
Authors:
 [1] ;  [2] ;  [1] ; ORCiD logo [3] ;  [3] ;  [4] ; ORCiD logo [1]
  1. National Autonomous University of Mexico (Mexico). Institute of Chemistry
  2. University of Guanajuato (Mexico). Department of Biology, Division of Natural and Exact Sciences
  3. Elettra Sincrotrone Trieste (Italy)
  4. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
Publication Date:
Report Number(s):
BNL-113790-2017-JA
Journal ID: ISSN 1528-7483
Grant/Contract Number:
SC0012704; AC02-76SF00515
Type:
Accepted Manuscript
Journal Name:
Crystal Growth and Design
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1528-7483
Publisher:
American Chemical Society
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 59 BASIC BIOLOGICAL SCIENCES; electric fields; magnetic fields; lysozyme; glucose isomerase; crystal growth; in situ data collection; protein crystallization
OSTI Identifier:
1392208