skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1390670
Grant/Contract Number:  
AC36-08-GO28308
Resource Type:
Published Article
Journal Name:
Biochimica et Biophysica Acta - Bioenergetics
Additional Journal Information:
Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Volume: 1857 Journal Issue: 1; Journal ID: ISSN 0005-2728
Publisher:
Elsevier
Country of Publication:
Netherlands
Language:
English

Citation Formats

Morra, Simone, Maurelli, Sara, Chiesa, Mario, Mulder, David W., Ratzloff, Michael W., Giamello, Elio, King, Paul W., Gilardi, Gianfranco, and Valetti, Francesca. The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation. Netherlands: N. p., 2016. Web. https://doi.org/10.1016/j.bbabio.2015.10.005.
Morra, Simone, Maurelli, Sara, Chiesa, Mario, Mulder, David W., Ratzloff, Michael W., Giamello, Elio, King, Paul W., Gilardi, Gianfranco, & Valetti, Francesca. The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation. Netherlands. https://doi.org/10.1016/j.bbabio.2015.10.005
Morra, Simone, Maurelli, Sara, Chiesa, Mario, Mulder, David W., Ratzloff, Michael W., Giamello, Elio, King, Paul W., Gilardi, Gianfranco, and Valetti, Francesca. Fri . "The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation". Netherlands. https://doi.org/10.1016/j.bbabio.2015.10.005.
@article{osti_1390670,
title = {The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation},
author = {Morra, Simone and Maurelli, Sara and Chiesa, Mario and Mulder, David W. and Ratzloff, Michael W. and Giamello, Elio and King, Paul W. and Gilardi, Gianfranco and Valetti, Francesca},
abstractNote = {},
doi = {10.1016/j.bbabio.2015.10.005},
journal = {Biochimica et Biophysica Acta - Bioenergetics},
number = 1,
volume = 1857,
place = {Netherlands},
year = {2016},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.bbabio.2015.10.005

Citation Metrics:
Cited by: 7 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

The active site of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans. II. Redox properties, light sensitivity and CO-ligand exchange as observed by infrared spectroscopy
journal, December 2005

  • Roseboom, Winfried; De Lacey, Antonio L.; Fernandez, Victor M.
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 11, Issue 1
  • DOI: 10.1007/s00775-005-0040-2

A novel FeS cluster in Fe-only hydrogenases
journal, March 2000

  • Nicolet, Yvain; Lemon, Brian J.; Fontecilla-Camps, Juan C.
  • Trends in Biochemical Sciences, Vol. 25, Issue 3, p. 138-143
  • DOI: 10.1016/S0968-0004(99)01536-4

Similarities in the Architecture of the Active Sites of Ni-Hydrogenases and Fe-Hydrogenases Detected by Means of Infrared Spectroscopy
journal, May 1996


Biomimetic assembly and activation of [FeFe]-hydrogenases
journal, June 2013

  • Berggren, G.; Adamska, A.; Lambertz, C.
  • Nature, Vol. 499, Issue 7456
  • DOI: 10.1038/nature12239

New Redox States Observed in [FeFe] Hydrogenases Reveal Redox Coupling Within the H-Cluster
journal, July 2014

  • Adamska-Venkatesh, Agnieszka; Krawietz, Danuta; Siebel, Judith
  • Journal of the American Chemical Society, Vol. 136, Issue 32
  • DOI: 10.1021/ja503390c

Metalloprotein mimics – old tools in a new light
journal, April 2014


The active site of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans. I. Light sensitivity and magnetic hyperfine interactions as observed by electron paramagnetic resonance
journal, December 2005

  • Albracht, Simon P. J.; Roseboom, Winfried; Hatchikian, E. Claude
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 11, Issue 1
  • DOI: 10.1007/s00775-005-0039-8

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli
journal, November 2010


Reevaluating the relationship between EPR spectra and enzyme structure for the iron sulfur clusters in NADH:quinone oxidoreductase
journal, July 2007

  • Yakovlev, G.; Reda, T.; Hirst, J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 31
  • DOI: 10.1073/pnas.0705593104

Molecular basis of [FeFe]-hydrogenase function
journal, August 2013

  • Winkler, Martin; Esselborn, Julian; Happe, Thomas
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 8-9
  • DOI: 10.1016/j.bbabio.2013.03.004

The reduced [2Fe-2S] clusters in adrenodoxin and Arthrospira platensis ferredoxin share spin density with protein nitrogens, probed using 2D ESEEM
journal, January 2009

  • Dikanov, Sergei A.; Samoilova, Rimma I.; Kappl, Reinhard
  • Physical Chemistry Chemical Physics, Vol. 11, Issue 31
  • DOI: 10.1039/b904597j

Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase
journal, April 2004

  • Posewitz, Matthew C.; King, Paul W.; Smolinski, Sharon L.
  • Journal of Biological Chemistry, Vol. 279, Issue 24
  • DOI: 10.1074/jbc.M403206200

[FeFe]-Hydrogenase Maturation
journal, June 2014

  • Shepard, Eric M.; Mus, Florence; Betz, Jeremiah N.
  • Biochemistry, Vol. 53, Issue 25
  • DOI: 10.1021/bi500210x

Designing interfaces of hydrogenase–nanomaterial hybrids for efficient solar conversion
journal, August 2013


Biological hydrogen production: prospects and challenges
journal, May 2010


De novo design of functional proteins: Toward artificial hydrogenases: De Novo Design of Functional Proteins
journal, November 2013

  • Faiella, Marina; Roy, Anindya; Sommer, Dayn
  • Biopolymers, Vol. 100, Issue 6
  • DOI: 10.1002/bip.22420

EPR and FTIR Analysis of the Mechanism of H 2 Activation by [FeFe]-Hydrogenase HydA1 from Chlamydomonas reinhardtii
journal, April 2013

  • Mulder, David W.; Ratzloff, Michael W.; Shepard, Eric M.
  • Journal of the American Chemical Society, Vol. 135, Issue 18
  • DOI: 10.1021/ja4000257

Identification and Characterization of the “Super-Reduced” State of the H-Cluster in [FeFe] Hydrogenase: A New Building Block for the Catalytic Cycle?
journal, October 2012

  • Adamska, Agnieszka; Silakov, Alexey; Lambertz, Camilla
  • Angewandte Chemie International Edition, Vol. 51, Issue 46
  • DOI: 10.1002/anie.201204800

Artificially maturated [FeFe] hydrogenase from Chlamydomonas reinhardtii: a HYSCORE and ENDOR study of a non-natural H-cluster
journal, January 2015

  • Adamska-Venkatesh, Agnieszka; Simmons, Trevor R.; Siebel, Judith F.
  • Physical Chemistry Chemical Physics, Vol. 17, Issue 7
  • DOI: 10.1039/C4CP05426A

A Novel [2Fe-2S] Ferredoxin from Pseudomonas putidamt2 Promotes the Reductive Reactivation of Catechol 2,3-Dioxygenase
journal, April 1998

  • Hugo, Nicolas; Armengaud, Jean; Gaillard, Jacques
  • Journal of Biological Chemistry, Vol. 273, Issue 16
  • DOI: 10.1074/jbc.273.16.9622

Molecular dynamics study of the proposed proton transport pathways in [FeFe]-hydrogenase
journal, January 2014

  • Ginovska-Pangovska, Bojana; Ho, Ming-Hsun; Linehan, John C.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 1
  • DOI: 10.1016/j.bbabio.2013.08.004

Electronic Structure of the H Cluster in [Fe]-Hydrogenases
journal, September 1999

  • Popescu, Codrina V.; Münck, Eckard
  • Journal of the American Chemical Society, Vol. 121, Issue 34
  • DOI: 10.1021/ja991243y

FeFe hydrogenase reductive inactivation and implication for catalysis
journal, January 2014

  • Hajj, Viviane; Baffert, Carole; Sybirna, Kateryna
  • Energy Environ. Sci., Vol. 7, Issue 2
  • DOI: 10.1039/C3EE42075B

Spin distribution of the H-cluster in the Hox–CO state of the [FeFe] hydrogenase from Desulfovibrio desulfuricans: HYSCORE and ENDOR study of 14N and 13C nuclear interactions
journal, November 2008

  • Silakov, Alexey; Wenk, Brian; Reijerse, Eduard
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 14, Issue 2
  • DOI: 10.1007/s00775-008-0449-5

Mechanism of Proton Transfer in [FeFe]-Hydrogenase from Clostridium pasteurianum
journal, September 2011

  • Cornish, Adam J.; Gärtner, Katrin; Yang, Hui
  • Journal of Biological Chemistry, Vol. 286, Issue 44
  • DOI: 10.1074/jbc.M111.254664

Isolation and first EPR characterization of the [FeFe]-hydrogenases from green algae
journal, May 2008

  • Kamp, Christina; Silakov, Alexey; Winkler, Martin
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1777, Issue 5
  • DOI: 10.1016/j.bbabio.2008.02.002

Cell-free H-cluster Synthesis and [FeFe] Hydrogenase Activation: All Five CO and CN− Ligands Derive from Tyrosine
journal, May 2011


Structure-Function Relationships in [FeFe]-Hydrogenase Active Site Maturation
journal, March 2012

  • Nicolet, Yvain; Fontecilla-Camps, Juan C.
  • Journal of Biological Chemistry, Vol. 287, Issue 17
  • DOI: 10.1074/jbc.R111.310797

Hydride Binding to the Active Site of [FeFe]-Hydrogenase
journal, October 2014

  • Chernev, Petko; Lambertz, Camilla; Brünje, Annika
  • Inorganic Chemistry, Vol. 53, Issue 22
  • DOI: 10.1021/ic502047q

Insights into metalloenzyme microenvironments: biomimetic metal complexes with a functional second coordination sphere
journal, January 2013

  • Zhao, Meng; Wang, Hai-Bo; Ji, Liang-Nian
  • Chemical Society Reviews, Vol. 42, Issue 21
  • DOI: 10.1039/c3cs60162e

The mechanisms of H2 activation and CO binding by hydrogenase I and hydrogenase II of Clostridium pasteurianum.
journal, November 1987


Crystallographic and FTIR Spectroscopic Evidence of Changes in Fe Coordination Upon Reduction of the Active Site of the Fe-Only Hydrogenase from Desulfovibrio d esulfuricans
journal, February 2001

  • Nicolet, Yvain; de Lacey, Antonio L.; Vernède, Xavier
  • Journal of the American Chemical Society, Vol. 123, Issue 8, p. 1596-1601
  • DOI: 10.1021/ja0020963

The Electronic Structure of the H-Cluster in the [FeFe]-Hydrogenase from Desulfovibrio desulfuricans : A Q-band 57 Fe-ENDOR and HYSCORE Study
journal, September 2007

  • Silakov, Alexey; Reijerse, Eduard J.; Albracht, Simon P. J.
  • Journal of the American Chemical Society, Vol. 129, Issue 37
  • DOI: 10.1021/ja072592s

The Cyanide Ligands of [FeFe] Hydrogenase: Pulse EPR Studies of 13 C and 15 N-Labeled H-Cluster
journal, August 2014

  • Myers, William K.; Stich, Troy A.; Suess, Daniel L. M.
  • Journal of the American Chemical Society, Vol. 136, Issue 35
  • DOI: 10.1021/ja507046w

Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007

  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

The quest for a functional substrate access tunnel in FeFe hydrogenase
journal, January 2011

  • Lautier, Thomas; Ezanno, Pierre; Baffert, Carole
  • Faraday Discuss., Vol. 148
  • DOI: 10.1039/C004099C

Reversible Carbon Monoxide Binding and Inhibition at the Active Site of the Fe-Only Hydrogenase
journal, June 2000

  • Bennett, Brian; Lemon, Brian J.; Peters, John W.
  • Biochemistry, Vol. 39, Issue 25
  • DOI: 10.1021/bi992583z

Investigations on the Role of Proton-Coupled Electron Transfer in Hydrogen Activation by [FeFe]-Hydrogenase
journal, October 2014

  • Mulder, David W.; Ratzloff, Michael W.; Bruschi, Maurizio
  • Journal of the American Chemical Society, Vol. 136, Issue 43
  • DOI: 10.1021/ja508629m

Activation and Inactivation of Hydrogenase Function and the Catalytic Cycle:  Spectroelectrochemical Studies
journal, October 2007

  • De Lacey, Antonio L.; Fernández, Víctor M.; Rousset, Marc
  • Chemical Reviews, Vol. 107, Issue 10
  • DOI: 10.1021/cr0501947

Biomimetic Chemistry of Iron, Nickel, Molybdenum, and Tungsten in Sulfur-Ligated Protein Sites
journal, March 2009

  • Groysman, Stanislav; Holm, R. H.
  • Biochemistry, Vol. 48, Issue 11
  • DOI: 10.1021/bi900044e

The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster
journal, March 2014

  • Fourmond, Vincent; Greco, Claudio; Sybirna, Kateryna
  • Nature Chemistry, Vol. 6, Issue 4
  • DOI: 10.1038/nchem.1892

A low-spin iron with CN and CO as intrinsic ligands forms the core of the active site in [Fe]-hydrogenases
journal, December 1998


An EPR and electron nuclear double resonance investigation of carbon monoxide binding to hydrogenase I (bidirectional) from Clostridium pasteurianum W5.
journal, October 1986


A Capable Bridging Ligand for Fe-Only Hydrogenase:  Density Functional Calculations of a Low-Energy Route for Heterolytic Cleavage and Formation of Dihydrogen
journal, April 2001

  • Fan, Hua-Jun; Hall, Michael B.
  • Journal of the American Chemical Society, Vol. 123, Issue 16
  • DOI: 10.1021/ja004120i

Spectroelectrochemical Characterization of the Active Site of the [FeFe] Hydrogenase HydA1 from Chlamydomonas reinhardtii
journal, August 2009

  • Silakov, Alexey; Kamp, Christina; Reijerse, Eduard
  • Biochemistry, Vol. 48, Issue 33
  • DOI: 10.1021/bi9009105

Dithiomethylether as a Ligand in the Hydrogenase H-Cluster
journal, April 2008

  • Pandey, Arti S.; Harris, Travis V.; Giles, Logan J.
  • Journal of the American Chemical Society, Vol. 130, Issue 13
  • DOI: 10.1021/ja711187e

Production of hydrogenases as biocatalysts
journal, October 2012


Biohydrogen production: prospects and limitations to practical application
journal, February 2004


The [4Fe–4S]-cluster coordination of [FeFe]-hydrogenase maturation protein HydF as revealed by EPR and HYSCORE spectroscopies
journal, December 2012

  • Berto, Paola; Di Valentin, Marilena; Cendron, Laura
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 12
  • DOI: 10.1016/j.bbabio.2012.09.004

Self-assembled biomimetic [2Fe2S]-hydrogenase-based photocatalyst for molecular hydrogen evolution
journal, January 2009

  • Kluwer, A. M.; Kapre, R.; Hartl, F.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 26
  • DOI: 10.1073/pnas.0809666106

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase
journal, April 2012


Structure/Function Relationships of [NiFe]- and [FeFe]-Hydrogenases
journal, October 2007

  • Fontecilla-Camps, Juan C.; Volbeda, Anne; Cavazza, Christine
  • Chemical Reviews, Vol. 107, Issue 10
  • DOI: 10.1021/cr050195z

Proton Transport in Clostridium pasteurianum [FeFe] Hydrogenase I: A Computational Study
journal, January 2014

  • Long, Hai; King, Paul W.; Chang, Christopher H.
  • The Journal of Physical Chemistry B, Vol. 118, Issue 4
  • DOI: 10.1021/jp408621r

EasySpin, a comprehensive software package for spectral simulation and analysis in EPR
journal, January 2006


Desulfovibrio desulfuricans iron hydrogenase: the structure shows unusual coordination to an active site Fe binuclear center
journal, January 1999


A Radical Intermediate in Tyrosine Scission to the CO and CN- Ligands of FeFe Hydrogenase
journal, October 2013


Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System
journal, March 2006


Hydrogenases
journal, March 2014

  • Lubitz, Wolfgang; Ogata, Hideaki; Rüdiger, Olaf
  • Chemical Reviews, Vol. 114, Issue 8
  • DOI: 10.1021/cr4005814

Optimized over-expression of [FeFe] hydrogenases with high specific activity in Clostridium acetobutylicum
journal, November 2008


An Isc-Type Extremely Thermostable [2Fe−2S] Ferredoxin from Aquifex aeolicus . Biochemical, Spectroscopic, and Unfolding Studies
journal, February 2003

  • Mitou, Géraldine; Higgins, Catherine; Wittung-Stafshede, Pernilla
  • Biochemistry, Vol. 42, Issue 5
  • DOI: 10.1021/bi027116n

14N HYSCORE investigation of the H-cluster of [FeFe] hydrogenase: evidence for a nitrogen in the dithiol bridge
journal, January 2009

  • Silakov, Alexey; Wenk, Brian; Reijerse, Eduard
  • Physical Chemistry Chemical Physics, Vol. 11, Issue 31
  • DOI: 10.1039/b905841a