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Title: Advances in microscale separations towards nanoproteomics applications

Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. But, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1 μg total proteins (e.g., cellular heterogeneity in tissue pathologies). We review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division, Environmental Molecular Sciences Lab.
Publication Date:
Report Number(s):
PNNL-SA-125498
Journal ID: ISSN 0021-9673; PII: S0021967317310567
Grant/Contract Number:
AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Journal of Chromatography
Additional Journal Information:
Journal Volume: 1523; Journal ID: ISSN 0021-9673
Publisher:
Elsevier
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Microscale separations; Nanoproteomics; NanoLC; Capillary electrophoresis; Mass spectrometry
OSTI Identifier:
1390579

Yi, Lian, Piehowski, Paul D., Shi, Tujin, Smith, Richard D., and Qian, Wei-Jun. Advances in microscale separations towards nanoproteomics applications. United States: N. p., Web. doi:10.1016/j.chroma.2017.07.055.
Yi, Lian, Piehowski, Paul D., Shi, Tujin, Smith, Richard D., & Qian, Wei-Jun. Advances in microscale separations towards nanoproteomics applications. United States. doi:10.1016/j.chroma.2017.07.055.
Yi, Lian, Piehowski, Paul D., Shi, Tujin, Smith, Richard D., and Qian, Wei-Jun. 2017. "Advances in microscale separations towards nanoproteomics applications". United States. doi:10.1016/j.chroma.2017.07.055. https://www.osti.gov/servlets/purl/1390579.
@article{osti_1390579,
title = {Advances in microscale separations towards nanoproteomics applications},
author = {Yi, Lian and Piehowski, Paul D. and Shi, Tujin and Smith, Richard D. and Qian, Wei-Jun},
abstractNote = {Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. But, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1 μg total proteins (e.g., cellular heterogeneity in tissue pathologies). We review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications.},
doi = {10.1016/j.chroma.2017.07.055},
journal = {Journal of Chromatography},
number = ,
volume = 1523,
place = {United States},
year = {2017},
month = {7}
}