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Title: A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux

Abstract

Lipid accumulation within the lumen of endolysosomal vesicles is observed in various pathologies including atherosclerosis, liver disease, neurological disorders, lysosomal storage disorders, and cancer. Current methods cannot measure lipid flux specifically within the lysosomal lumen of live cells. We developed an optical reporter, composed of a photoluminescent carbon nanotube of a single chirality, that responds to lipid accumulation via modulation of the nanotube’s optical band gap. The engineered nanomaterial, composed of short, single-stranded DNA and a single nanotube chirality, localizes exclusively to the lumen of endolysosomal organelles without adversely affecting cell viability or proliferation or organelle morphology, integrity, or function. The emission wavelength of the reporter can be spatially resolved from within the endolysosomal lumen to generate quantitative maps of lipid content in live cells. Endolysosomal lipid accumulation in cell lines, an example of drug-induced phospholipidosis, was observed for multiple drugs in macrophages, and measurements of patient-derived Niemann–Pick type C fibroblasts identified lipid accumulation and phenotypic reversal of this lysosomal storage disease. Single-cell measurements using the reporter discerned subcellular differences in equilibrium lipid content, illuminating significant intracellular heterogeneity among endolysosomal organelles of differentiating bone-marrow-derived monocytes. Single-cell kinetics of lipoprotein-derived cholesterol accumulation within macrophages revealed rates that differed among cells bymore » an order of magnitude. This carbon nanotube optical reporter of endolysosomal lipid content in live cells confers additional capabilities for drug development processes and the investigation of lipid-linked diseases.« less

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3];  [4]; ORCiD logo [3];  [5]; ORCiD logo [1];  [5];  [5]; ORCiD logo [3];  [6];  [5];  [7]; ORCiD logo [3]
  1. Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States
  2. Department of Chemical Engineering, University of Rhode Island, Kingston, Rhode Island 02881, United States
  3. Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States, Weill Cornell Medicine, New York, New York 10065, United States
  4. Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States, Division of Tumor Biology &, Immunology, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
  5. Weill Cornell Medicine, New York, New York 10065, United States
  6. Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, Pennsylvania 18015, United States
  7. Memorial Sloan Kettering Cancer Center, New York, New York 10065, United States, Weill Cornell Medicine, New York, New York 10065, United States, Ludwig Center for Cancer Research, University of Lausanne, Lausanne CH 1066, Switzerland
Publication Date:
Research Org.:
Memorial Sloan Kettering Cancer Center, New York, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division
OSTI Identifier:
1389696
Alternate Identifier(s):
OSTI ID: 1507740
Grant/Contract Number:  
[SC0013979; AC02-05CH11231]
Resource Type:
Published Article
Journal Name:
ACS Nano
Additional Journal Information:
[Journal Name: ACS Nano Journal Volume: 11 Journal Issue: 11]; Journal ID: ISSN 1936-0851
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 36 MATERIALS SCIENCE; 42 ENGINEERING; hyperspectral microscopy; live-cell imaging; near-infrared fluorescence; single-cell sensing; single-walled carbon nanotubes

Citation Formats

Jena, Prakrit V., Roxbury, Daniel, Galassi, Thomas V., Akkari, Leila, Horoszko, Christopher P., Iaea, David B., Budhathoki-Uprety, Januka, Pipalia, Nina, Haka, Abigail S., Harvey, Jackson D., Mittal, Jeetain, Maxfield, Frederick R., Joyce, Johanna A., and Heller, Daniel A. A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux. United States: N. p., 2017. Web. doi:10.1021/acsnano.7b04743.
Jena, Prakrit V., Roxbury, Daniel, Galassi, Thomas V., Akkari, Leila, Horoszko, Christopher P., Iaea, David B., Budhathoki-Uprety, Januka, Pipalia, Nina, Haka, Abigail S., Harvey, Jackson D., Mittal, Jeetain, Maxfield, Frederick R., Joyce, Johanna A., & Heller, Daniel A. A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux. United States. doi:10.1021/acsnano.7b04743.
Jena, Prakrit V., Roxbury, Daniel, Galassi, Thomas V., Akkari, Leila, Horoszko, Christopher P., Iaea, David B., Budhathoki-Uprety, Januka, Pipalia, Nina, Haka, Abigail S., Harvey, Jackson D., Mittal, Jeetain, Maxfield, Frederick R., Joyce, Johanna A., and Heller, Daniel A. Tue . "A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux". United States. doi:10.1021/acsnano.7b04743.
@article{osti_1389696,
title = {A Carbon Nanotube Optical Reporter Maps Endolysosomal Lipid Flux},
author = {Jena, Prakrit V. and Roxbury, Daniel and Galassi, Thomas V. and Akkari, Leila and Horoszko, Christopher P. and Iaea, David B. and Budhathoki-Uprety, Januka and Pipalia, Nina and Haka, Abigail S. and Harvey, Jackson D. and Mittal, Jeetain and Maxfield, Frederick R. and Joyce, Johanna A. and Heller, Daniel A.},
abstractNote = {Lipid accumulation within the lumen of endolysosomal vesicles is observed in various pathologies including atherosclerosis, liver disease, neurological disorders, lysosomal storage disorders, and cancer. Current methods cannot measure lipid flux specifically within the lysosomal lumen of live cells. We developed an optical reporter, composed of a photoluminescent carbon nanotube of a single chirality, that responds to lipid accumulation via modulation of the nanotube’s optical band gap. The engineered nanomaterial, composed of short, single-stranded DNA and a single nanotube chirality, localizes exclusively to the lumen of endolysosomal organelles without adversely affecting cell viability or proliferation or organelle morphology, integrity, or function. The emission wavelength of the reporter can be spatially resolved from within the endolysosomal lumen to generate quantitative maps of lipid content in live cells. Endolysosomal lipid accumulation in cell lines, an example of drug-induced phospholipidosis, was observed for multiple drugs in macrophages, and measurements of patient-derived Niemann–Pick type C fibroblasts identified lipid accumulation and phenotypic reversal of this lysosomal storage disease. Single-cell measurements using the reporter discerned subcellular differences in equilibrium lipid content, illuminating significant intracellular heterogeneity among endolysosomal organelles of differentiating bone-marrow-derived monocytes. Single-cell kinetics of lipoprotein-derived cholesterol accumulation within macrophages revealed rates that differed among cells by an order of magnitude. This carbon nanotube optical reporter of endolysosomal lipid content in live cells confers additional capabilities for drug development processes and the investigation of lipid-linked diseases.},
doi = {10.1021/acsnano.7b04743},
journal = {ACS Nano},
number = [11],
volume = [11],
place = {United States},
year = {2017},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1021/acsnano.7b04743

Citation Metrics:
Cited by: 12 works
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Figures / Tables:

Figure 1. Figure 1.: Optical response of carbon nanotube complexes to lipid environments. (a) Normalized absorption and emission spectra of ss(GT)6−carbon nanotube complexes purified to isolate the (8,6) species. (b) Emission peak wavelength of ss(GT)6-(8,6) nanotube complexes in solution as a function of cholesterol-PEG concentration. Error bars are standard error of themore » mean, obtained from three technical replicates performed for each concentration. (c) Mean emission wavelength of ss(GT)6-(8,6) nanotube complexes exposed to different solvents. Error bars are standard errors of the mean, obtained from five technical replicates for each solvent. (d) Frames from allatom molecular dynamics simulations of equilibrated structures of the ss(GT)6-(8,6) nanotube complex in water and the same complex equilibrated in the presence of cholesterol or sphingomyelin. (e) Water molecule density as a function of distance from the surface of the equilibrated ss(GT)6-(8,6) nanotube complex and the same complex equilibrated in the presence of cholesterol or sphingomyelin.« less

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.