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Title: A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging

Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with 41,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. We report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with B50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. Additionally, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [1] ;  [1] ;  [1] ;  [3] ; ORCiD logo [1] ;  [3] ;  [3] ; ORCiD logo [4] ;  [1] ;  [3]
  1. Stanford Univ., CA (United States). Dept. of Chemistry
  2. Wuhan Univ. (China). State Key Lab. of Virology, Key Lab. of Combinatorial Biosynthesis and Drug Discovery; Stanford Univ., CA (United States). Molecular Imaging Program (MIPS), Dept. of Radiology, Canary Center at Stanford for Cancer Early Detection
  3. Stanford Univ., CA (United States). Molecular Imaging Program (MIPS), Dept. of Radiology, Canary Center at Stanford for Cancer Early Detection
  4. Wuhan Univ. (China). State Key Lab. of Virology, Key Lab. of Combinatorial Biosynthesis and Drug Discovery
Publication Date:
Grant/Contract Number:
SC0008397
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Stanford Univ., CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 60 APPLIED LIFE SCIENCES; biomedical materials; fluorescence imaging; supramolecular chemistry
OSTI Identifier:
1389584