A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

doi:10.1105/tpc.16.00855

Title: A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

Abstract

Flavonoids represent a large family of specialized metabolites involved in plant growth, development, and adaptation. Chalcone synthase (CHS) catalyzes the first step of flavonoid biosynthesis by directing carbon flux from general phenylpropanoid metabolism to flavonoid pathway. Despite extensive characterization of its function and transcriptional regulation, the molecular basis governing its posttranslational modification is enigmatic. Here, we report the discovery of a proteolytic regulator of CHS, namely, KFB ^CHS, a Kelch domain-containing F-box protein in Arabidopsis thaliana. KFB ^CHS physically interacts with CHS and specifically mediates its ubiquitination and degradation. KFB ^CHS exhibits developmental expression patterns in Arabidopsis leaves, stems, and siliques and strongly responds to the dark-to-light (or the light-to-dark) switch, the blue, red, and far-red light signals, and UV-B irradiation. Alteration of KFB ^CHS expression negatively correlates to the cellular concentration of CHS and the production of flavonoids. Our study suggests that KFB ^CHS serves as a crucial negative regulator, via mediating CHS degradation, coordinately controlling flavonoid biosynthesis in response to the developmental cues and environmental stimuli.

Authors:

^[1]; Abrahan, Carolina ^[2]; Colquhoun, Thomas A. ^[2];

^[1]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Univ. of Florida, Gainesville, FL (United States)

Publication Date:: 2017-04-26

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

OSTI Identifier:: 1389244

Report Number(s):: BNL-114250-2017-JA
Journal ID: ISSN 1040-4651

Grant/Contract Number:: SC0012704

Resource Type:: Accepted Manuscript

Journal Name:: Plant Cell

Additional Journal Information:: Journal Volume: 29; Journal Issue: 5; Journal ID: ISSN 1040-4651

Publisher:: American Society of Plant Biologists

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Flavonoids; Chalcone synthase; F-box protein; Ubiquitination; Arabidopsis

Citation Formats


                    Zhang, Xuebin, Abrahan, Carolina, Colquhoun, Thomas A., and Liu, Chang-Jun. A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis.  United States: N. p., 2017. 
        Web.  doi:10.1105/tpc.16.00855.

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                    Zhang, Xuebin, Abrahan, Carolina, Colquhoun, Thomas A., & Liu, Chang-Jun. A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis.  United States.  doi:10.1105/tpc.16.00855.

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                    Zhang, Xuebin, Abrahan, Carolina, Colquhoun, Thomas A., and Liu, Chang-Jun. Wed .  
        "A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis".  United States.  doi:10.1105/tpc.16.00855.  https://www.osti.gov/servlets/purl/1389244.

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@article{osti_1389244,

  title        = {A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis},

  author       = {Zhang, Xuebin and Abrahan, Carolina and Colquhoun, Thomas A. and Liu, Chang-Jun},

  abstractNote = {Flavonoids represent a large family of specialized metabolites involved in plant growth, development, and adaptation. Chalcone synthase (CHS) catalyzes the first step of flavonoid biosynthesis by directing carbon flux from general phenylpropanoid metabolism to flavonoid pathway. Despite extensive characterization of its function and transcriptional regulation, the molecular basis governing its posttranslational modification is enigmatic. Here, we report the discovery of a proteolytic regulator of CHS, namely, KFBCHS, a Kelch domain-containing F-box protein in Arabidopsis thaliana. KFBCHS physically interacts with CHS and specifically mediates its ubiquitination and degradation. KFBCHS exhibits developmental expression patterns in Arabidopsis leaves, stems, and siliques and strongly responds to the dark-to-light (or the light-to-dark) switch, the blue, red, and far-red light signals, and UV-B irradiation. Alteration of KFBCHS expression negatively correlates to the cellular concentration of CHS and the production of flavonoids. Our study suggests that KFBCHS serves as a crucial negative regulator, via mediating CHS degradation, coordinately controlling flavonoid biosynthesis in response to the developmental cues and environmental stimuli.},

  doi          = {10.1105/tpc.16.00855},

  journal      = {Plant Cell},

  number       = 5,

  volume       = 29,

  place        = {United States},

  year         = {2017},

  month        = {4}

}

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DOI: 10.1105/tpc.16.00855

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Chalcone synthases (CHSs): the symbolic type III polyketide synthases
journal, November 2019

Pandith, Shahzad A.; Ramazan, Salika; Khan, Mohd Ishfaq
Planta, Vol. 251, Issue 1
DOI: 10.1007/s00425-019-03307-y

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L
journal, December 2019

Zhang, Shulin; Tian, Zailong; Li, Haipeng
BMC Genomics, Vol. 20, Issue 1
DOI: 10.1186/s12864-019-6280-2

Chalcone synthases (CHSs): the symbolic type III polyketide synthases
journal, November 2019

Pandith, Shahzad A.; Ramazan, Salika; Khan, Mohd Ishfaq
Planta, Vol. 251, Issue 1
DOI: 10.1007/s00425-019-03307-y

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L
journal, December 2019

Zhang, Shulin; Tian, Zailong; Li, Haipeng
BMC Genomics, Vol. 20, Issue 1
DOI: 10.1186/s12864-019-6280-2

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Abstract

Citation Formats

Chalcone synthases (CHSs): the symbolic type III polyketide synthases journal, November 2019

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L journal, December 2019

Chalcone synthases (CHSs): the symbolic type III polyketide synthases journal, November 2019

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L journal, December 2019

Chalcone synthases (CHSs): the symbolic type III polyketide synthases
journal, November 2019

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L
journal, December 2019

Chalcone synthases (CHSs): the symbolic type III polyketide synthases
journal, November 2019

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L
journal, December 2019