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Title: A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

Journal Article · · The Plant Cell
ORCiD logo [1];  [2];  [2]; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Univ. of Florida, Gainesville, FL (United States)
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Flavonoids represent a large family of specialized metabolites involved in plant growth, development, and adaptation. Chalcone synthase (CHS) catalyzes the first step of flavonoid biosynthesis by directing carbon flux from general phenylpropanoid metabolism to flavonoid pathway. Despite extensive characterization of its function and transcriptional regulation, the molecular basis governing its posttranslational modification is enigmatic. Here, we report the discovery of a proteolytic regulator of CHS, namely, KFBCHS, a Kelch domain-containing F-box protein in Arabidopsis thaliana. KFBCHS physically interacts with CHS and specifically mediates its ubiquitination and degradation. KFBCHS exhibits developmental expression patterns in Arabidopsis leaves, stems, and siliques and strongly responds to the dark-to-light (or the light-to-dark) switch, the blue, red, and far-red light signals, and UV-B irradiation. Alteration of KFBCHS expression negatively correlates to the cellular concentration of CHS and the production of flavonoids. Our study suggests that KFBCHS serves as a crucial negative regulator, via mediating CHS degradation, coordinately controlling flavonoid biosynthesis in response to the developmental cues and environmental stimuli.

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Grant/Contract Number:
SC0012704
OSTI ID:
1389244
Report Number(s):
BNL--114250-2017-JA
Journal Information:
The Plant Cell, Journal Name: The Plant Cell Journal Issue: 5 Vol. 29; ISSN 1040-4651
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (27)

Chalcone synthases (CHSs): the symbolic type III polyketide synthases journal November 2019
Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus journal December 2019
Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L journal December 2019
Isolation and characterization of kelch repeat-containing F-box proteins from colored wheat journal January 2020
Expression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus journal December 2019
Low Temperature-Enhanced Flavonol Synthesis Requires Light-Associated Regulatory Components in Arabidopsis thaliana journal July 2018
UV regulates the expression of phenylpropanoid biosynthesis genes in cucumber ( Cucumis sativus L.) in an organ and spectrum dependent manner journal January 2019
Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are auxiliary components in prenylated flavonoid biosynthesis journal May 2018
Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are auxiliary components in prenylated flavonoid biosynthesis journal May 2018
Plant Fibers and Phenolics: A Review on Their Synthesis, Analysis and Combined Use for Biomaterials with New Properties journal August 2019
Comparative transcriptome analysis provides global insight into gene expression differences between two orchid cultivars journal July 2018
Regulation of color transition in purple tea (Camellia sinensis) journal December 2019
Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L journal December 2019
Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome‐dependent degradation of PAL journal September 2019
Pollen development at high temperature and role of carbon and nitrogen metabolites journal June 2019
The dynamic response of the Arabidopsis root metabolome to auxin and ethylene is not predicted by changes in the transcriptome journal January 2020
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’ journal May 2019
Genome-Wide Identification and Analysis of U-Box E3 Ubiquitin–Protein Ligase Gene Family in Banana journal December 2018
Post-translational and transcriptional regulation of phenylpropanoid biosynthesis pathway by Kelch repeat F-box protein SAGL1 journal December 2018
Identification and distribution of a single nucleotide polymorphism responsible for the catechin content in tea plants journal March 2020
LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening journal April 2019
TRANSPARENT TESTA 4 -mediated flavonoids negatively affect embryonic fatty acid biosynthesis in Arabidopsis: Flavonoids negatively affect embryonic fatty acid biosynthesis journal August 2018
LcNAC13 Physically Interacts with LcR1MYB1 to Coregulate Anthocyanin Biosynthesis-Related Genes during Litchi Fruit Ripening journal April 2019
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’ journal May 2019
Additional file 1 of Genome-wide identification, evolutionary and functional analyses of KFB family members in potato dataset January 2022
Genome-wide transcript analysis of inflorescence development in wheat journal September 2019
Genome-Wide Identification and Analysis of U-Box E3 Ubiquitin–Protein Ligase Gene Family in Banana journal December 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Flavonoids
Chalcone synthase
F-box protein
Ubiquitination
Arabidopsis