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Title: Carotenoid-to-Bacteriochlorophyll Energy Transfer in the LH1–RC Core Complex of a Bacteriochlorophyll b Containing Purple Photosynthetic Bacterium Blastochloris viridis

Abstract

Carotenoid-to-bacteriochlorophyll energy transfer has been widely investigated in bacteriochlorophyll (BChl) $$a$$-containing light harvesting complexes. Blastochloris viridis utilizes BChl b, whose absorption spectrum is more red-shifted than that of BChl α. This has implications on the efficiency and pathways of carotenoid-to-BChl energy transfer in this organism. The carotenoids that comprise the light-harvesting reaction center core complex (LH1–RC) of B. viridis are 1,2-dihydroneurosporene and 1,2-dihydrolycopene, which are derivatives of carotenoids found in the light harvesting complexes of several BChl α-containing purple photosynthetic bacteria. Steady-state and ultrafast time-resolved optical spectroscopic measurements were performed on the LH1–RC complex of B. at room and cryogenic temperatures. The overall efficiency of carotenoid-to-bacteriochlorophyll energy transfer obtained from steady-state absorption and fluorescence measurements were determined to be ~27% and ~36% for 1,2-dihydroneurosporene and 1,2-dihydrolycopene, respectively. These results were combined with global fitting and target analyses of the transient absorption data to elucidate the energetic pathways by which the carotenoids decay and transfer excitation energy to BChl b. 1,2-Dihydrolycopene transfers energy to BChl b via the S2 → Qx channel with $$k$$ET2 = (500 fs)-1 while 1,2-dihydroneurosporene transfers energy via S1→ Qy ($$k$$ET1 = (84 ps)-1) and S2 → Qx ($$k$$ET2 = (2.2 ps)-1) channels.

Authors:
 [1];  [1];  [2];  [1]
+ Show Author Affiliations
  1. Washington Univ., St. Louis, MO (United States). Photosynthetic Antenna Research Center
  2. Washington Univ., St. Louis, MO (United States)
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1387879
Grant/Contract Number:  
SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry
Additional Journal Information:
Journal Volume: 120; Journal Issue: 23; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 1520-6106
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly)

Citation Formats

Magdaong, Nikki Cecil M., Niedzwiedzki, Dariusz M., Goodson, Carrie, and Blankenship, Robert E. Carotenoid-to-Bacteriochlorophyll Energy Transfer in the LH1–RC Core Complex of a Bacteriochlorophyll b Containing Purple Photosynthetic Bacterium Blastochloris viridis. United States: N. p., 2016. Web. doi:10.1021/acs.jpcb.6b04307.
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Magdaong, Nikki Cecil M., Niedzwiedzki, Dariusz M., Goodson, Carrie, & Blankenship, Robert E. Carotenoid-to-Bacteriochlorophyll Energy Transfer in the LH1–RC Core Complex of a Bacteriochlorophyll b Containing Purple Photosynthetic Bacterium Blastochloris viridis. United States. https://doi.org/10.1021/acs.jpcb.6b04307
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Magdaong, Nikki Cecil M., Niedzwiedzki, Dariusz M., Goodson, Carrie, and Blankenship, Robert E. Tue . "Carotenoid-to-Bacteriochlorophyll Energy Transfer in the LH1–RC Core Complex of a Bacteriochlorophyll b Containing Purple Photosynthetic Bacterium Blastochloris viridis". United States. https://doi.org/10.1021/acs.jpcb.6b04307. https://www.osti.gov/servlets/purl/1387879.
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@article{osti_1387879,
title = {Carotenoid-to-Bacteriochlorophyll Energy Transfer in the LH1–RC Core Complex of a Bacteriochlorophyll b Containing Purple Photosynthetic Bacterium Blastochloris viridis},
author = {Magdaong, Nikki Cecil M. and Niedzwiedzki, Dariusz M. and Goodson, Carrie and Blankenship, Robert E.},
abstractNote = {Carotenoid-to-bacteriochlorophyll energy transfer has been widely investigated in bacteriochlorophyll (BChl) $a$-containing light harvesting complexes. Blastochloris viridis utilizes BChl b, whose absorption spectrum is more red-shifted than that of BChl α. This has implications on the efficiency and pathways of carotenoid-to-BChl energy transfer in this organism. The carotenoids that comprise the light-harvesting reaction center core complex (LH1–RC) of B. viridis are 1,2-dihydroneurosporene and 1,2-dihydrolycopene, which are derivatives of carotenoids found in the light harvesting complexes of several BChl α-containing purple photosynthetic bacteria. Steady-state and ultrafast time-resolved optical spectroscopic measurements were performed on the LH1–RC complex of B. at room and cryogenic temperatures. The overall efficiency of carotenoid-to-bacteriochlorophyll energy transfer obtained from steady-state absorption and fluorescence measurements were determined to be ~27% and ~36% for 1,2-dihydroneurosporene and 1,2-dihydrolycopene, respectively. These results were combined with global fitting and target analyses of the transient absorption data to elucidate the energetic pathways by which the carotenoids decay and transfer excitation energy to BChl b. 1,2-Dihydrolycopene transfers energy to BChl b via the S2 → Qx channel with $k$ET2 = (500 fs)-1 while 1,2-dihydroneurosporene transfers energy via S1→ Qy ($k$ET1 = (84 ps)-1) and S2 → Qx ($k$ET2 = (2.2 ps)-1) channels.},
doi = {10.1021/acs.jpcb.6b04307},
journal = {Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry},
number = 23,
volume = 120,
place = {United States},
year = {Tue May 24 00:00:00 EDT 2016},
month = {Tue May 24 00:00:00 EDT 2016}
}
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https://doi.org/10.1021/acs.jpcb.6b04307
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