Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples
- Authors:
-
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
- California Inst. of Technology (CalTech), Pasadena, CA (United States). Division of Geological and Planetary Sciences
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
- OSTI Identifier:
- 1395289
- Alternate Identifier(s):
- OSTI ID: 1379954; OSTI ID: 1413502
- Report Number(s):
- PNNL-SA-125645
Journal ID: ISSN 1387-3806; PII: S1387380617302130
- Grant/Contract Number:
- AC02-05CH11231; AC05-76RL01830; P41 GM103493; R21 EB020976-01A1; 1R21EB020976-01A1
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Mass Spectrometry
- Additional Journal Information:
- Journal Volume: 427; Journal ID: ISSN 1387-3806
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Ultrasensitive; NanoLC; Orbitrap Fusion Lumos; Match between runs; Subnanogram proteomics; Small cell populations; 97 MATHEMATICS AND COMPUTING; ultrasensitive; nanoLC; match between runs; subnanogram proteomics; small cell populations
Citation Formats
Zhu, Ying, Zhao, Rui, Piehowski, Paul D., Moore, Ronald J., Lim, Sujung, Orphan, Victoria J., Paša-Tolić, Ljiljana, Qian, Wei-Jun, Smith, Richard D., and Kelly, Ryan T. Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples. United States: N. p., 2017.
Web. doi:10.1016/J.IJMS.2017.08.016.
Zhu, Ying, Zhao, Rui, Piehowski, Paul D., Moore, Ronald J., Lim, Sujung, Orphan, Victoria J., Paša-Tolić, Ljiljana, Qian, Wei-Jun, Smith, Richard D., & Kelly, Ryan T. Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples. United States. https://doi.org/10.1016/J.IJMS.2017.08.016
Zhu, Ying, Zhao, Rui, Piehowski, Paul D., Moore, Ronald J., Lim, Sujung, Orphan, Victoria J., Paša-Tolić, Ljiljana, Qian, Wei-Jun, Smith, Richard D., and Kelly, Ryan T. Fri .
"Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples". United States. https://doi.org/10.1016/J.IJMS.2017.08.016. https://www.osti.gov/servlets/purl/1395289.
@article{osti_1395289,
title = {Subnanogram proteomics: Impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples},
author = {Zhu, Ying and Zhao, Rui and Piehowski, Paul D. and Moore, Ronald J. and Lim, Sujung and Orphan, Victoria J. and Paša-Tolić, Ljiljana and Qian, Wei-Jun and Smith, Richard D. and Kelly, Ryan T.},
abstractNote = {},
doi = {10.1016/J.IJMS.2017.08.016},
journal = {International Journal of Mass Spectrometry},
number = ,
volume = 427,
place = {United States},
year = {Fri Sep 01 00:00:00 EDT 2017},
month = {Fri Sep 01 00:00:00 EDT 2017}
}
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Figures / Tables:
Fig. 1: Comparison of LC column size on the performance of proteomic analysis. (a1–a2) Base peak chromatograms of 10-ng tryptic digest of Shewanella oneidensis with (a1)75-μm and (a2) 30-μm i.d. LC columns and a LTQ-Orbitrap MS. Y axis was fixed at 8E6 to show the signal gain for the 30-μmmore »
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