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Title: Anaerobic Digestion of Organic Fraction from Hydrothermal Liquefied Algae Wastewater Byproduct

We present that the wastewater stream from hydrothermal liquefaction (HTL) process used in biofuel production, contains a large amounts of organic compounds where several can be regarded as environmentally hazardous and requires significant treatment before disposal. In this study, semi-continuous anaerobic digestion is used to degrade the organic fraction of wastewater streams from HTL of the algae Tetraselmis (AgTet) and Chlorella (AgChlr). Results indicated high methane yields at 20-30% (v/v) HTL wastewater together with clarified manure, producing 327.2 mL/gVS in (or volatile solids in feed) for AgTet and 263.4 mL/gVS in for AgChlr. There was a significant reduction in methane production at concentrations higher than 40% (v/v) HTL wastewater in the feed, possibly due to the accumulation of chloride salts or inhibitory compounds such as pyridines, piperidines and pyrrolidines. In conclusion, this was further confirmed by comparing COD, salt and the ammonia concentrations of the effluents after anaerobic digestion at different concentrations of wastewater in manure.
 [1] ;  [1] ;  [2] ;  [2] ;  [3]
  1. Washington State University, Richland, WA (United States). Bioproducts, Sciences and Engineering Laboratory
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Chemical and Biological Processes Development Group, Energy and Efficiency Division
  3. Washington State University, Richland, WA (United States). Bioproducts, Sciences and Engineering Laboratory; Washington State University, Pullman, WA (United States). Biological Systems Engineering, L.J. Smith Hall and The Gene and Linda Voiland School of Chemical Engineering and Bioengineering
Publication Date:
Grant/Contract Number:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 247; Journal ID: ISSN 0960-8524
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Anaerobic Digestion; Chlorella; Hydrothermal liquefaction; Methane; Testraselmis; Wastewater treatment
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1395277