skip to main content

DOE PAGESDOE PAGES

Title: Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar

Biomass pretreatment remains an essential step in lignocellulosic biofuel production, largely to facilitate the efficient removal of lignin and increase enzyme accessibility to the polysaccharides. In recent years, there have been significant efforts in planta to reduce lignin content or modify its composition to overcome the inherent recalcitrance that it imposes on lignocellulosic biomass during processing. Here, transgenic poplar lines in which monolignol ferulate conjugates were synthesized during cell wall development to introduce, during lignification, readily cleavable ester linkages into the lignin polymer backbone (i.e., "zip lignin"), along with wild-type (WT) controls, were pretreated with different ionic liquids (ILs). The strategic introduction of ester bonds into the lignin backbone resulted in increased pretreatment efficiency and released more carbohydrates with lower energy input. After pretreatment with any of three different ILs, and after limited saccharification, the transgenic poplars, especially those with relatively higher amounts of incorporated monolignol ferulate conjugates, yielded up to 23% higher sugar levels compared to WT plants. Our findings clearly demonstrate that the introduction of ester linkages into the lignin polymer backbone decreases biomass recalcitrance in poplar has the potential to reduce the energy and/or amount of IL required for effective pretreatment, and could enable the development ofmore » an economically viable and sustainable biorefinery process.« less
Authors:
 [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [1]
  1. Joint BioEnergy Institute, Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Univ. of Wisconsin, Madison, WI (United States); Wisconsin Energy Institute, Madison, WI (United States)
  3. Wisconsin Energy Institute, Madison, WI (United States); Univ. of British Columbia, Vancouver (Canada)
  4. Joint BioEnergy Institute, Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Biofuels; Biomass; Lignocellulose
OSTI Identifier:
1379811

Kim, Kwang Ho, Dutta, Tanmoy, Ralph, John, Mansfield, Shawn D., Simmons, Blake A., and Singh, Seema. Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar. United States: N. p., Web. doi:10.1186/s13068-017-0784-2.
Kim, Kwang Ho, Dutta, Tanmoy, Ralph, John, Mansfield, Shawn D., Simmons, Blake A., & Singh, Seema. Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar. United States. doi:10.1186/s13068-017-0784-2.
Kim, Kwang Ho, Dutta, Tanmoy, Ralph, John, Mansfield, Shawn D., Simmons, Blake A., and Singh, Seema. 2017. "Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar". United States. doi:10.1186/s13068-017-0784-2. https://www.osti.gov/servlets/purl/1379811.
@article{osti_1379811,
title = {Impact of lignin polymer backbone esters on ionic liquid pretreatment of poplar},
author = {Kim, Kwang Ho and Dutta, Tanmoy and Ralph, John and Mansfield, Shawn D. and Simmons, Blake A. and Singh, Seema},
abstractNote = {Biomass pretreatment remains an essential step in lignocellulosic biofuel production, largely to facilitate the efficient removal of lignin and increase enzyme accessibility to the polysaccharides. In recent years, there have been significant efforts in planta to reduce lignin content or modify its composition to overcome the inherent recalcitrance that it imposes on lignocellulosic biomass during processing. Here, transgenic poplar lines in which monolignol ferulate conjugates were synthesized during cell wall development to introduce, during lignification, readily cleavable ester linkages into the lignin polymer backbone (i.e., "zip lignin"), along with wild-type (WT) controls, were pretreated with different ionic liquids (ILs). The strategic introduction of ester bonds into the lignin backbone resulted in increased pretreatment efficiency and released more carbohydrates with lower energy input. After pretreatment with any of three different ILs, and after limited saccharification, the transgenic poplars, especially those with relatively higher amounts of incorporated monolignol ferulate conjugates, yielded up to 23% higher sugar levels compared to WT plants. Our findings clearly demonstrate that the introduction of ester linkages into the lignin polymer backbone decreases biomass recalcitrance in poplar has the potential to reduce the energy and/or amount of IL required for effective pretreatment, and could enable the development of an economically viable and sustainable biorefinery process.},
doi = {10.1186/s13068-017-0784-2},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {4}
}

Works referenced in this record:

Toward Thermoplastic Lignin Polymers. Part 1. Selective Masking of Phenolic Hydroxyl Groups in Kraft Lignins via Methylation and Oxypropylation Chemistries
journal, December 2012
  • Sadeghifar, Hasan; Cui, Chengzhong; Argyropoulos, Dimitris S.
  • Industrial & Engineering Chemistry Research, Vol. 51, Issue 51, p. 16713-16720
  • DOI: 10.1021/ie301848j

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment
journal, July 2009

Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
journal, April 2014

Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy
journal, November 1996

Visualization of biomass solubilization and cellulose regeneration during ionic liquid pretreatment of switchgrass
journal, September 2009
  • Singh, Seema; Simmons, Blake A.; Vogel, Kenneth P.
  • Biotechnology and Bioengineering, Vol. 104, Issue 1, p. 68-75
  • DOI: 10.1002/bit.22386