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Title: Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici

Abstract

The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the native PA-producing bacterium Propionibacterium acidipropionici. A wide range of culture conditions and process parameters were examined and experimentally optimized to maximize titer, rate, and yield of PA. The effect of gas sparging during fermentation was first examined, and N 2 was found to exhibit improved performance over CO 2. Subsequently, the effects of different hydrolysate concentrations, nitrogen sources, and neutralization agents were investigated. One of the best combinations found during batch experiments used yeast extract (YE) as the primary nitrogen source and NH 4OH for pH control. This combination enabled PA titers of 30.8 g/L with a productivity of 0.40 g/L h from 76.8 g/L biomass sugars, while successfully minimizing lactic acid production. Due to the economic significance of downstream separations, increasing titers using fed-batch fermentation was examined by changing both feeding media and strategy. Continuous feeding of hydrolysate was found to be superior to pulsed feeding and combined with high YE concentrations increased PA titers to 62.7 g/L and improvedmore » the simultaneous utilization of different biomass sugars. Additionally, applying high YE supplementation maintains the lactic acid concentration below 4 g/L for the duration of the fermentation. Finally, with the aim of increasing productivity, high cell density fed-batch fermentations were conducted. PA titers increased to 64.7 g/L with a productivity of 2.35 g/L h for the batch stage and 0.77 g/L h for the overall process. These results highlight the importance of media and fermentation strategy to improve PA production. Altogether, this work demonstrates the feasibility of producing PA from corn stover hydrolysate.« less

Authors:
 [1]; ORCiD logo [1];  [1];  [1];  [1];  [2]; ORCiD logo [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Colorado School of Mines, Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1379461
Report Number(s):
NREL/JA-5100-70061
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; lignocellulosic biomass; fermentation; biochemicals; biorefinery; organic acids

Citation Formats

Wang, Xiaoqing, Salvachua, Davinia, Sanchez i Nogue, Violeta, Michener, William E., Bratis, Adam D., Dorgan, John R., and Beckham, Gregg T. Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0884-z.
Wang, Xiaoqing, Salvachua, Davinia, Sanchez i Nogue, Violeta, Michener, William E., Bratis, Adam D., Dorgan, John R., & Beckham, Gregg T. Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici. United States. doi:10.1186/s13068-017-0884-z.
Wang, Xiaoqing, Salvachua, Davinia, Sanchez i Nogue, Violeta, Michener, William E., Bratis, Adam D., Dorgan, John R., and Beckham, Gregg T. Thu . "Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici". United States. doi:10.1186/s13068-017-0884-z. https://www.osti.gov/servlets/purl/1379461.
@article{osti_1379461,
title = {Propionic acid production from corn stover hydrolysate by Propionibacterium acidipropionici},
author = {Wang, Xiaoqing and Salvachua, Davinia and Sanchez i Nogue, Violeta and Michener, William E. and Bratis, Adam D. and Dorgan, John R. and Beckham, Gregg T.},
abstractNote = {The production of value-added chemicals alongside biofuels from lignocellulosic hydrolysates is critical for developing economically viable biorefineries. Here, the production of propionic acid (PA), a potential building block for C3-based chemicals, from corn stover hydrolysate is investigated using the native PA-producing bacterium Propionibacterium acidipropionici. A wide range of culture conditions and process parameters were examined and experimentally optimized to maximize titer, rate, and yield of PA. The effect of gas sparging during fermentation was first examined, and N2 was found to exhibit improved performance over CO2. Subsequently, the effects of different hydrolysate concentrations, nitrogen sources, and neutralization agents were investigated. One of the best combinations found during batch experiments used yeast extract (YE) as the primary nitrogen source and NH4OH for pH control. This combination enabled PA titers of 30.8 g/L with a productivity of 0.40 g/L h from 76.8 g/L biomass sugars, while successfully minimizing lactic acid production. Due to the economic significance of downstream separations, increasing titers using fed-batch fermentation was examined by changing both feeding media and strategy. Continuous feeding of hydrolysate was found to be superior to pulsed feeding and combined with high YE concentrations increased PA titers to 62.7 g/L and improved the simultaneous utilization of different biomass sugars. Additionally, applying high YE supplementation maintains the lactic acid concentration below 4 g/L for the duration of the fermentation. Finally, with the aim of increasing productivity, high cell density fed-batch fermentations were conducted. PA titers increased to 64.7 g/L with a productivity of 2.35 g/L h for the batch stage and 0.77 g/L h for the overall process. These results highlight the importance of media and fermentation strategy to improve PA production. Altogether, this work demonstrates the feasibility of producing PA from corn stover hydrolysate.},
doi = {10.1186/s13068-017-0884-z},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {8}
}

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Works referenced in this record:

Prefermentation improves xylose utilization in simultaneous saccharification and co-fermentation of pretreated spruce
journal, January 2009

  • Bertilsson, Magnus; Olofsson, Kim; Lidén, Gunnar
  • Biotechnology for Biofuels, Vol. 2, Article No. 8
  • DOI: 10.1186/1754-6834-2-8

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011