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Title: High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India

Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [3] ;  [3] ;  [4] ;  [4] ;  [5] ;  [5] ;  [3] ;  [6] ;  [2]
  1. J.N. Vyas Univ., Jodhpur (India). Dept. of Botany
  2. Murdoch Univ., WA (Australia). Centre for Studies
  3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Data Management and Technology Center
  5. King Abdulaziz Univ., Jeddah (Saudi Arabia). Dept. of Biological Sciences
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia). Dept. of Biological Sciences
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Standards in Genomic Sciences
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1944-3277
Publisher:
BioMed Central
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; root-nodule bacteria; nitrogen fixation; symbiosis; ensifer; prosopis
OSTI Identifier:
1379405

Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India. United States: N. p., Web. doi:10.1186/s40793-016-0157-7.
Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, & Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India. United States. doi:10.1186/s40793-016-0157-7.
Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. 2016. "High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India". United States. doi:10.1186/s40793-016-0157-7. https://www.osti.gov/servlets/purl/1379405.
@article{osti_1379405,
title = {High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India},
author = {Gehlot, Hukam Singh and Ardley, Julie and Tak, Nisha and Tian, Rui and Poonar, Neetu and Meghwal, Raju R. and Rathi, Sonam and Tiwari, Ravi and Adnawani, Wan and Seshadri, Rekha and Reddy, T. B. K. and Pati, Amrita and Woyke, Tanja and Pillay, Manoj and Markowitz, Victor and Baeshen, Mohammed N. and Al-Hejin, Ahmed M. and Ivanova, Natalia and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.},
doi = {10.1186/s40793-016-0157-7},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 11,
place = {United States},
year = {2016},
month = {6}
}

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