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Title: Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology: Metagenomic investigation of the Hellenic Volcanic Arc

Hydrothermal vents represent a deep, hot, aphotic biosphere where chemosynthetic primary producers, fuelled by chemicals from Earth's subsurface, form the basis of life. In this study, we examined microbial mats from two distinct volcanic sites within the Hellenic Volcanic Arc (HVA). The HVA is geologically and ecologically unique, with reported emissions of CO 2-saturated fluids at temperatures up to 220°C and a notable absence of macrofauna. Metagenomic data reveals highly complex prokaryotic communities composed of chemolithoautotrophs, some methanotrophs, and to our surprise, heterotrophs capable of anaerobic degradation of aromatic hydrocarbons. Our data suggest that aromatic hydrocarbons may indeed be a significant source of carbon in these sites, and instigate additional research into the nature and origin of these compounds in the HVA. Novel physiology was assigned to several uncultured prokaryotic lineages; most notably, a SAR406 representative is attributed with a role in anaerobic hydrocarbon degradation. This dataset, the largest to date from submarine volcanic ecosystems, constitutes a significant resource of novel genes and pathways with potential biotechnological applications.
Authors:
 [1] ;  [1] ;  [2] ;  [2] ;  [1] ;  [2] ;  [2] ;  [3] ;  [4] ;  [5] ;  [4] ;  [1] ;  [1] ;  [1] ;  [2] ;  [6]
  1. Hellenic Centre for Marine Research, Crete (Greece)
  2. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  4. National and Kapodistrian Univ. of Athens (Greece)
  5. Univ. of Rhode Island, Kingston, RI (United States)
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); King Abdulaziz Univ., Jeddah (Saudi Arabia)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Environmental Microbiology
Additional Journal Information:
Journal Volume: 18; Journal Issue: 4; Journal ID: ISSN 1462-2912
Publisher:
Wiley
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1379265

Oulas, Anastasis, Polymenakou, Paraskevi N., Seshadri, Rekha, Tripp, H. James, Mandalakis, Manolis, Paez-Espino, A. David, Pati, Amrita, Chain, Patrick, Nomikou, Paraskevi, Carey, Steven, Kilias, Stephanos, Christakis, Christos, Kotoulas, Georgios, Magoulas, Antonios, Ivanova, Natalia N., and Kyrpides, Nikos C.. Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology: Metagenomic investigation of the Hellenic Volcanic Arc. United States: N. p., Web. doi:10.1111/1462-2920.13095.
Oulas, Anastasis, Polymenakou, Paraskevi N., Seshadri, Rekha, Tripp, H. James, Mandalakis, Manolis, Paez-Espino, A. David, Pati, Amrita, Chain, Patrick, Nomikou, Paraskevi, Carey, Steven, Kilias, Stephanos, Christakis, Christos, Kotoulas, Georgios, Magoulas, Antonios, Ivanova, Natalia N., & Kyrpides, Nikos C.. Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology: Metagenomic investigation of the Hellenic Volcanic Arc. United States. doi:10.1111/1462-2920.13095.
Oulas, Anastasis, Polymenakou, Paraskevi N., Seshadri, Rekha, Tripp, H. James, Mandalakis, Manolis, Paez-Espino, A. David, Pati, Amrita, Chain, Patrick, Nomikou, Paraskevi, Carey, Steven, Kilias, Stephanos, Christakis, Christos, Kotoulas, Georgios, Magoulas, Antonios, Ivanova, Natalia N., and Kyrpides, Nikos C.. 2015. "Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology: Metagenomic investigation of the Hellenic Volcanic Arc". United States. doi:10.1111/1462-2920.13095. https://www.osti.gov/servlets/purl/1379265.
@article{osti_1379265,
title = {Metagenomic investigation of the geologically unique Hellenic Volcanic Arc reveals a distinctive ecosystem with unexpected physiology: Metagenomic investigation of the Hellenic Volcanic Arc},
author = {Oulas, Anastasis and Polymenakou, Paraskevi N. and Seshadri, Rekha and Tripp, H. James and Mandalakis, Manolis and Paez-Espino, A. David and Pati, Amrita and Chain, Patrick and Nomikou, Paraskevi and Carey, Steven and Kilias, Stephanos and Christakis, Christos and Kotoulas, Georgios and Magoulas, Antonios and Ivanova, Natalia N. and Kyrpides, Nikos C.},
abstractNote = {Hydrothermal vents represent a deep, hot, aphotic biosphere where chemosynthetic primary producers, fuelled by chemicals from Earth's subsurface, form the basis of life. In this study, we examined microbial mats from two distinct volcanic sites within the Hellenic Volcanic Arc (HVA). The HVA is geologically and ecologically unique, with reported emissions of CO2-saturated fluids at temperatures up to 220°C and a notable absence of macrofauna. Metagenomic data reveals highly complex prokaryotic communities composed of chemolithoautotrophs, some methanotrophs, and to our surprise, heterotrophs capable of anaerobic degradation of aromatic hydrocarbons. Our data suggest that aromatic hydrocarbons may indeed be a significant source of carbon in these sites, and instigate additional research into the nature and origin of these compounds in the HVA. Novel physiology was assigned to several uncultured prokaryotic lineages; most notably, a SAR406 representative is attributed with a role in anaerobic hydrocarbon degradation. This dataset, the largest to date from submarine volcanic ecosystems, constitutes a significant resource of novel genes and pathways with potential biotechnological applications.},
doi = {10.1111/1462-2920.13095},
journal = {Environmental Microbiology},
number = 4,
volume = 18,
place = {United States},
year = {2015},
month = {12}
}