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Title: Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs

Abstract

Biomineralized ferric oxide microbial mats are ubiquitous features on Earth, are common in hot springs of Yellowstone National Park (YNP, WY, USA), and form due to direct interaction between microbial and physicochemical processes. The overall goal of this study was to determine the contribution of different community members to the assembly and succession of acidic high-temperature Fe(III)-oxide mat ecosystems. Spatial and temporal changes in Fe(III)-oxide accretion and the abundance of relevant community members were monitored over 70 days using sterile glass microscope slides incubated in the outflow channels of two acidic geothermal springs (pH = 3-3.5; temperature = 68-75°C) in YNP. Hydrogenobaculum spp. were the most abundant taxon identified during early successional stages (4-40 days), and have been shown to oxidize arsenite, sulfide, and hydrogen coupled to oxygen reduction. Iron-oxidizing populations of Metallosphaera yellowstonensis were detected within 4 days, and reached steady-state levels within 14-30 days, corresponding to visible Fe(III)-oxide accretion. Heterotrophic archaea colonized near 30 days, and emerged as the dominant functional guild after 70 days and in mature Fe(III)-oxide mats (1-2 cm thick). First-order rate constants of Fe(III)-oxide accretion ranged from 0.046 to 0.05 day -1 , and in situ microelectrode measurements showed that the oxidation of Fe(II)more » is limited by the diffusion of O2 into the Fe(III)-oxide mat. The formation of microterracettes also implicated O2 as a major variable controlling microbial growth and subsequent mat morphology. The assembly and succession of Fe(III)-oxide mat communities follows a repeatable pattern of colonization by lithoautotrophic organisms, and the subsequent growth of diverse organoheterotrophs. The unique geochemical signatures and micromorphology of extant biomineralized Fe(III)-oxide mats are also useful for understanding other Fe(II)-oxidizing systems.« less

Authors:
 [1];  [2];  [3];  [1];  [1];  [4];  [1]
  1. Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences
  2. Montana State Univ., Bozeman, MT (United States). Dept. of Chemical and Biological Engineering; Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biodetection Science and Biological Science Division
  3. Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences, Dept. of Chemical and Biological Engineering
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1379092
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 7; Journal Issue: FEB; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; hydrogenobaculum; metallosphaera; lithoautotroph; organoheterotroph; archaea; biomineralization; oxygen

Citation Formats

Beam, Jacob P., Bernstein, Hans C., Jay, Zackary J., Kozubal, Mark A., Jennings, Ryan deM., Tringe, Susannah G., and Inskeep, William P. Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs. United States: N. p., 2016. Web. doi:10.3389/fmicb.2016.00025.
Beam, Jacob P., Bernstein, Hans C., Jay, Zackary J., Kozubal, Mark A., Jennings, Ryan deM., Tringe, Susannah G., & Inskeep, William P. Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs. United States. doi:10.3389/fmicb.2016.00025.
Beam, Jacob P., Bernstein, Hans C., Jay, Zackary J., Kozubal, Mark A., Jennings, Ryan deM., Tringe, Susannah G., and Inskeep, William P. Mon . "Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs". United States. doi:10.3389/fmicb.2016.00025. https://www.osti.gov/servlets/purl/1379092.
@article{osti_1379092,
title = {Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs},
author = {Beam, Jacob P. and Bernstein, Hans C. and Jay, Zackary J. and Kozubal, Mark A. and Jennings, Ryan deM. and Tringe, Susannah G. and Inskeep, William P.},
abstractNote = {Biomineralized ferric oxide microbial mats are ubiquitous features on Earth, are common in hot springs of Yellowstone National Park (YNP, WY, USA), and form due to direct interaction between microbial and physicochemical processes. The overall goal of this study was to determine the contribution of different community members to the assembly and succession of acidic high-temperature Fe(III)-oxide mat ecosystems. Spatial and temporal changes in Fe(III)-oxide accretion and the abundance of relevant community members were monitored over 70 days using sterile glass microscope slides incubated in the outflow channels of two acidic geothermal springs (pH = 3-3.5; temperature = 68-75°C) in YNP. Hydrogenobaculum spp. were the most abundant taxon identified during early successional stages (4-40 days), and have been shown to oxidize arsenite, sulfide, and hydrogen coupled to oxygen reduction. Iron-oxidizing populations of Metallosphaera yellowstonensis were detected within 4 days, and reached steady-state levels within 14-30 days, corresponding to visible Fe(III)-oxide accretion. Heterotrophic archaea colonized near 30 days, and emerged as the dominant functional guild after 70 days and in mature Fe(III)-oxide mats (1-2 cm thick). First-order rate constants of Fe(III)-oxide accretion ranged from 0.046 to 0.05 day -1 , and in situ microelectrode measurements showed that the oxidation of Fe(II) is limited by the diffusion of O2 into the Fe(III)-oxide mat. The formation of microterracettes also implicated O2 as a major variable controlling microbial growth and subsequent mat morphology. The assembly and succession of Fe(III)-oxide mat communities follows a repeatable pattern of colonization by lithoautotrophic organisms, and the subsequent growth of diverse organoheterotrophs. The unique geochemical signatures and micromorphology of extant biomineralized Fe(III)-oxide mats are also useful for understanding other Fe(II)-oxidizing systems.},
doi = {10.3389/fmicb.2016.00025},
journal = {Frontiers in Microbiology},
number = FEB,
volume = 7,
place = {United States},
year = {2016},
month = {2}
}

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